Detection of poxtA- and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm

Ruiz-Ripa, Laura; Feßler, Andrea T.; Hanke, Dennis; Cervera, Susana Sanz; Olarte, Carmen; Eichhorn, Inga; Schwarz, Štefan; Torres, Carmén

doi:10.1016/j.jgar.2019.12.012

Cited by 25 publications

(28 citation statements)

References 17 publications

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“…The wide antimicrobial resistance found suggests that the environment may play an important role in the spread of MDR E. faecium isolates (Hamiwe et al 2019;Ripa et al 2020). On the other hand, E. faecium isolates are important nosocomial pathogens, and resistance to several antimicrobial classes is closely related to a higher mortality rate since therapeutic options have become limited in the treatment of enterococcal infections (Golob et al 2019;Igbinosa and Beshiru 2019).…”

Section: Resultsmentioning

confidence: 99%

Occurrence of multidrug‐resistantEnterococcus faeciumisolated from environmental samples

Santos

Furlan

Gallo

et al. 2021

Lett Appl Microbiol

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Section: Resultsmentioning

confidence: 99%

Occurrence of multidrug‐resistantEnterococcus faeciumisolated from environmental samples

Santos

Furlan

Gallo

et al. 2021

Lett Appl Microbiol

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“…The presence of 34 antimicrobial resistance genes was investigated by PCR: mecA, blaZ, erm(A), erm(B), erm(C), erm(T), mph(C), msr(A), msr(B), lnu(A), vga(A), vga(C), aacA-aphD, aphA3, aadE, aadD, aadA, str, tet(K), tet(M), tet(L), sat4 (even though streptothricin susceptibility was not tested), fexA, fexB, cfr, optrA, poxtA, cat ps194 , cat pC221 , cat pC223 , dfrA, dfrD, dfrG, and dfrK [18,39,40]. Positive controls from the collection of the University of La Rioja were included in all PCR assays.…”

Section: Antimicrobial Resistance Phenotype and Genotypementioning

confidence: 99%

S. pseudintermedius and S. aureus lineages with transmission ability circulate as causative agents of infections in pets for years

et al. 2021

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Background Staphylococcus pseudintermedius (SP) and Staphylococcus aureus (SA) are common colonizers of companion animals, but they are also considered opportunistic pathogens, causing diseases of diverse severity. This study focused on the identification and characterization of 33 coagulase-positive staphylococci isolated from diseased pets (28 dogs and five cats) during 2009–2011 in a veterinary hospital in Spain in order to stablish the circulating lineages and their antimicrobial resistance profile. Results Twenty-eight isolates were identified as SP and five as SA. Nine methicillin-resistant (MR) isolates (27%) carrying the mecA gene were detected (eight MRSP and one MRSA). The 55% of SP and SA isolates were multidrug-resistant (MDR). MRSP strains were typed as ST71-agrIII-SCCmecII/III-(PFGE) A (n=5), ST68-agrIV-SCCmecV-B1/B2 (n=2), and ST258-agrII-SCCmecIV-C (n=1). SP isolates showed resistance to the following antimicrobials [percentage of resistant isolates/resistance genes]: penicillin [82/blaZ], oxacillin [29/mecA] erythromycin/clindamycin [43/erm(B)], aminoglycosides [18–46/aacA-aphD, aphA3, aadE], tetracycline [71/tet(M), tet(K)], ciprofloxacin [29], chloramphenicol [29/catpC221], and trimethoprim-sulfamethoxazole [50/dfrG, dfrK]. The dfrK gene was revealed as part of the radC-integrated Tn559 in two SP isolates. Virulence genes detected among SP isolates were as follow [percentage of isolates]: siet [100], se-int [100], lukS/F-I [100], seccanine [7], and expB [7]. The single MRSA-mecA detected was typed as t011-ST398/CC398-agrI-SCCmecV and was MDR. The methicillin-susceptible SA isolates were typed as t045-ST5/CC5 (n=2), t10576-ST1660 (n=1), and t005-ST22/CC22 (n=1); the t005-ST22 feline isolate was PVL-positive and the two t045-ST45 isolates were ascribed to Immune Evasion Cluster (IEC) type F. Moreover, the t10576-ST1660 isolate, of potential equine origin, harbored the lukPQ and scneq genes. According to animal clinical history and data records, several strains seem to have been acquired from different sources of the hospital environment, while some SA strains appeared to have a human origin. Conclusions The frequent detection of MR and MDR isolates among clinical SP and SA strains with noticeable virulence traits is of veterinary concern, implying limited treatment options available. This is the first description of MRSA-ST398 and MRSP-ST68 in pets in Spain, as well the first report of the dfrK-carrying Tn559 in SP. This evidences that current transmissible lineages with mobilizable resistomes have been circulating as causative agents of infections among pets for years.

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“…All S. aureus isolates were subjected to spa-typing, following standard methodology (Ruiz-Ripa et al 2020b), and the obtained sequences were analysed using Ridom â Staph Type software (Ridom GmbH, M€ unster, Germany). The adscription to the clonal complex CC398 was performed by a specific PCR targeting the sau1-hsdS1 variant of this lineage (Stegger et al 2011).…”

Section: Bacterial Collection and Molecular Typingmentioning

confidence: 99%

“…The adscription to the clonal complex CC398 was performed by a specific PCR targeting the sau1-hsdS1 variant of this lineage (Stegger et al 2011). The isolates that were not ascribed to the CC398 were subjected to multilocus sequence typing as previously described (Ruiz-Ripa et al 2020b). The agr-typing was performed in all S. aureus isolates, and MRSA isolates were subjected to SCCmectyping (Zhang et al 2005;Kondo et al 2007;Ruiz-Ripa et al 2020c).…”

Section: Bacterial Collection and Molecular Typingmentioning

confidence: 99%

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

et al. 2021

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Aims: To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018-2019. Methods and Results: The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular techniques. The transferability of the cfr gene was assessed by conjugation and its genetic environment was determined by PCR mapping. In all, 21 isolates were methicillin-resistant S. aureus (MRSA) carrying the mecA gene (SCCmecV or non-typeable SCCmec), whereas the remaining two were methicillin-susceptible (MSSA). All but one MRSA isolates (n = 20) belonged to the CC398, being the spa t011 the most prevalent (n = 11). The remaining MRSA and the two MSSA isolates were ascribed to ST9/CC9. The S. aureus isolates exhibited resistance to (number of resistant isolates): b-lactamics (21), erythromycin and/or clindamycin (20), aminoglycosides (7), tetracycline (22), fluoroquinolones (14), chloramphenicol (5) and linezolid (1). The S. aureus isolates did not carry any of the virulence genes studied. One MRSA belonging to the CC398 showed linezolid resistance mediated by the cfr gene. The cfr gene was co-located with fexA in the Tn558 variant previously reported in the S. aureus plasmid pSCFS7. Conclusions: Two major livestock-associated genetic lineages were detected among pigs with signs of infection in Spain. The presence of the cfr gene among LA-MRSA-CC398 is of great concern not only for veterinary medicine, but also for humans in close contact. Significance and Impact of the Study: This work describes the molecular characterization of S. aureus isolates recovered from pigs with signs of infection and we report, as far as we know, the first description of MRSA-CC9 from pigs in Spain. Moreover, the detection of a MRSA-CC398 isolate carrying the multiresistance cfr gene highlights the need for continuous surveillance and awareness of LA-MRSA.

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Detection of poxtA- and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm

Cited by 25 publications

References 17 publications

Occurrence of multidrug‐resistantEnterococcus faeciumisolated from environmental samples

Occurrence of multidrug‐resistantEnterococcus faeciumisolated from environmental samples

S. pseudintermedius and S. aureus lineages with transmission ability circulate as causative agents of infections in pets for years

Linezolid‐resistant MRSA‐CC398 carrying the cfr gene, and MRSA‐CC9 isolates from pigs with signs of infection in Spain

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