Detection of potential biomarkers associated with outrageous diseases and environmental pollutants by nanoparticle-based immuno-PCR assays

Dahiya, Bhawna; Mehta, Promod K

doi:10.1016/j.ab.2019.113444

Cited by 28 publications

(9 citation statements)

References 109 publications

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“…Magnetic microspheres (MMP) are considered to be an ideal adsorption carrier to replace microplates. The liquid phase system of MMP provides uniform adsorption sites for the target. , By increasing the reaction dynamics, nonspecific binding is reduced, and detection accuracy can be improved. In addition, MMP can increase the detection sensitivity through magnetic enrichment.…”

Section: Immunoassay and Nucleic Acid Amplification Combined Technolo...mentioning

confidence: 99%

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Sang

Cheng

et al. 2021

J. Agric. Food Chem.

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An immunoassay is mostly employed for the direct detection of food contaminants, and a molecular assay for targeting nucleic acids employs amplification techniques for distinguishing genes. The integration of an immunoassay with nucleic acid amplification techniques inherits the direct and rapid performance of an immunoassay and the ultrasensitive merit of a molecular assay. Enthusiastic attention has been attracted in recent years on the utilization of isothermal amplification techniques in an immunoassay, as well as the employment of a lateral flow immunoassay in a molecular assay. Thus, this Review discussed these kinds of approaches from two categories: immuno-nucleic acid amplification (I-NAA) and nucleic acid amplification-immunoassay (NAA-I). The advantages, drawbacks, and future developments were discussed for a comprehensive understanding.

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Section: Immunoassay and Nucleic Acid Amplification Combined Technolo...mentioning

confidence: 99%

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Sang

Cheng

et al. 2021

J. Agric. Food Chem.

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“…Immuno-polymerase chain reaction (immuno-PCR) is a relatively novel and highly sensitive detection method to measure the trace amount of analytes in biological solutions [1]. Immuno-PCR combines the specificity of enzyme-linked immunosorbent assays (ELISA) with the sensitivity of PCR and has been proven useful for early-stage detection of various pathological conditions, for detection of contaminants in processed food, and for the environmental pollutants in liquid samples [2][3][4][5][6]. Despite the high sensitivity, immuno-PCR is a laborious, tedious, and time-consuming technique [7].…”

Section: Introductionmentioning

confidence: 99%

Size and macromolecule stabilizer–dependent performance of gold colloids in immuno-PCR

Tabatabaei

Islam²,

Ahmed

2022

Anal Bioanal Chem

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Gold nanoparticles (GNPs) are well-documented for their size and surface chemistry-dependent electronic and optical properties that are extensively utilized to develop highly sensitive immunoassays. GNP-based immuno-polymerase chain reaction (immuno-PCR) is especially interesting due to the facile loading of biomolecules on the surface of GNP probes and has been utilized to develop analyte-specific assays. In this study, the role of size and surface chemistry of GNPs is explored in detail to develop a highly sensitive and reproducible immuno-PCR assay for specific detection of biotinylated analytes. Our results indicate that smaller-sized gold nanoparticles outperform the larger ones in terms of their sensitivity in immuno-PCR assay and show superior loading of proteins and oligonucleotides on the surface of nanoparticles. Furthermore, the role of different macromolecular stabilizers (such as polyethylene glycol (PEG), bovine serum albumin (BSA), and PEGylated BSA) was compared to optimize the loading of biomolecules and to improve the signal-to-noise ratio of GNP probes. mPEG-BSAfunctionalized GNP probes of 15 nm were found to be highly sensitive at low concentrations of analytes and significantly (~ 30 fold) improve the limit of detection of analytes in comparison with ELISA assay.

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“…Only over the last decade this technique appeared to become more applicable in protein science. Possible reasons might be that DNA-antibody coupling, modified oligonucleotide labeling, and detailed protocols became accessible to a broader range of researchers [ 27 – 31 ]. Nowadays, the basic technique of immuno-PCR evolved into different sub-techniques adapted to their intended use.…”

Section: Introductionmentioning

confidence: 99%

Exploring solid-phase proximity ligation assay for survivin detection in urine

et al. 2022

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Urine-based biomarkers are a rational and promising approach for the detection of bladder cancer due to the proximity of urine to the location of the tumor site and the non-invasive nature of its sampling. A well-known and highly investigated biomarker for bladder cancer is survivin. For detection of very small amounts of urinary survivin protein a highly sensitive assay was developed. The assay is based on the immuno-PCR technology, more precisely a solid-phase proximity ligation assay (spPLA). The limit of detection for the survivin spPLA was 1.45 pg/mL, resulting in an improvement of the limit of detection by a factor of approximately 23 compared to the previously in-house developed survivin ELISA. A key step in development was the initial isolation of survivin by a molecular fishing rod based on magnetic beads. Interfering matrix compounds pose a special challenge for further analytical application, but can be overcome by this isolation step. The assay is designed to work with only 500 μL of voided urine. The survivin spPLA showed a sensitivity of 30% and specificity of 89% for bladder cancer detection in this study of 110 bladder cancer cases and 133 clinical controls. Moreover, the results demonstrated again that survivin is a useful complementary marker in combination with UBC® Rapid by increasing the overall sensitivity to 70% with a specificity of 86%. Although the performance for detection of bladder cancer was rather low, the herein developed assay might serve as a new tool for survivin biomarker research in diverse human fluids, even if the biological matrix is complex or survivin is only present in small amounts.

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Detection of potential biomarkers associated with outrageous diseases and environmental pollutants by nanoparticle-based immuno-PCR assays

Cited by 28 publications

References 109 publications

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Size and macromolecule stabilizer–dependent performance of gold colloids in immuno-PCR

Exploring solid-phase proximity ligation assay for survivin detection in urine

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