Detection of porcine teschoviruses and enteroviruses by LightCycler real-time PCR

Krumbholz, Andi; Wurm, R.; Scheck, Oliver; Birch-Hirschfeld, Eckhard; Egerer, Renate; Henke, Andreas; Wutzler, Peter; Zell, Roland

doi:10.1016/s0166-0934(03)00227-1

Cited by 54 publications

(44 citation statements)

References 16 publications

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“…Outbreaks of CSF cause heavy losses in pig production and severely hamper the international trade in livestock [1]. Previous studies have been reported that CSFV and PRRSV may cause disease with similar clinical symptoms [26]. Additionally, CSFV co-infection with PRRSV played an important role in reproducing typical post weaning multisystemic wasting syndrome [27].…”

Section: Discussionmentioning

confidence: 99%

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Wongsawat¹,

Dharaku²,

Narat³

et al. 2011

Health

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Classical swine fever virus (CSFV) is the causative agent of Classical swine fever which is a highly contagious disease affecting swine and resulting in severe economic losses. In this study, we developed reverse transcription loopmediated isothermal amplification (RT-LAMP) assay targeting the 5'UTR gene for the detection of CSFV. This amplification method can be obtained in 1 h under isothermal conditions (65°C) employing a set of six specific primers mixtures. Amplification product was visualized by using hydroxynaphthol blue (HNB) dye and agarose gel electrophoresis. The sensitivity was 100 copy numbers. No cross-reactivity related to Japanese encephalitis virus (JEV) and porcine reproductive and respiratory syndrome virus (PR-RSV) was demonstrated. The results demonstrated that the RT-LAMP assay is a useful tool for the rapid and sensitive for CSFV detection in swine.

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Section: Discussionmentioning

confidence: 99%

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Wongsawat¹,

Dharaku²,

Narat³

et al. 2011

Health

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“…Animal-specific enterovirus infections in hosts such as cattle and pigs are often asymptomatic but may cause diseases ranging from diarrhea to reproductive failure and neurological disorders (Table 1) (92,97). Two bovine enteroviruses (BEV), three porcine enteroviruses (PEV), 11 porcine teschoviruses (PTV) (10 were formerly classified as porcine enteroviruses), and 1 ovine enterovirus have been identified (87).…”

Section: Enterovirusesmentioning

confidence: 99%

Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools

Fong

Lipp

2005

Microbiol Mol Biol Rev

612

450

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Waterborne enteric viruses threaten both human and animal health. These pathogens are host specific and cause a wide range of diseases and symptoms in humans or other animals. While considerable research has documented the risk of enteric viruses to human health from contact with contaminated water, the current bacterial indicator-based methods for evaluation of water quality are often ineffectual proxies for pathogenic viruses. Additionally, relatively little work has specifically investigated the risk of waterborne viruses to animal health, and this risk currently is not addressed by routine water quality assessments. Nonetheless, because of their host specificity, enteric viruses can fulfill a unique role both for assessing health risks and as measures of contamination source in a watershed, yet the use of animal, as well as human, host-specific viruses in determining sources of fecal pollution has received little attention. With improved molecular detection assays, viruses from key host groups can be targeted directly using PCR amplification or hybridization with a high level of sensitivity and specificity. A multispecies viral analysis would provide needed information for controlling pollution by source, determining human health risks based on assessments of human virus loading and exposure, and determining potential risks to production animal health and could indicate the potential for the presence of other zoonotic pathogens. While there is a need to better understand the prevalence and environmental distribution of nonhuman enteric viruses, the development of improved methods for specific and sensitive detection will facilitate the use of these microbes for library-independent source tracking and water quality assessment tools

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“…14 The reverse transcription reaction was carried out at 50uC for 30 min, followed by an initial PCR activation step at 95uC for 15 min. The PCR reactions were amplified through 35 cycles.…”

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confidence: 99%

Porcine Teschovirus Polioencephalomyelitis in Western Canada

Salles

Scholes²,

Dauber

et al. 2011

J VET Diagn Invest

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Abstract. Beginning in 2002, a small number of pig farms in western Canada began reporting 4-7-weekold pigs with bilateral hind-end paresis or paralysis. Low numbers of pigs were affected, some died, most had to be euthanized, and those that survived had reduced weight gains and neurological deficits. Necropsies revealed no gross lesions, but microscopic lesions consisted of a nonsuppurative polioencephalomyelitis, most severe in the brain stem and spinal cord. The lesions were most consistent with a viral infection. Tests for circovirus, Porcine reproductive and respiratory syndrome virus, coronavirus, Rabies virus, and Pseudorabies virus were negative. Using immunohistochemistry, virus neutralization, fluorescent antibody test, and nested reverse transcription polymerase chain reaction, Porcine teschovirus was identified in tissues from affected individuals. To the authors' knowledge, this is the first report of teschovirus encephalitis in western Canada and the first reported case of polioencephalomyelitis in pigs in Canada, where teschovirus was confirmed as the cause.

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Detection of porcine teschoviruses and enteroviruses by LightCycler real-time PCR

Cited by 54 publications

References 16 publications

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Detection of nucleic acid of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools

Porcine Teschovirus Polioencephalomyelitis in Western Canada

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