Detection of polycyclic aromatic hydrocarbon degradation genes in different soil bacteria by polymerase chain reaction and DNA hybridization

Hamann, Christian S.; Hegemann, JÃ¶rg; Hildebrandt, A.

doi:10.1111/j.1574-6968.1999.tb13510.x

Cited by 85 publications

(27 citation statements)

References 28 publications

(33 reference statements)

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“…Fresh insights into HMW PAH biodegradation, such as the observation that fluoranthene is anaerobically oxidized to carbon dioxide under sulfate-reducing conditions in ocean sediments (22), show that knowledge in the field of PAH biodeg- radation is expanding in new directions. Advances in molecular biology are aiding in the detection of PAH-degrading organisms from environmental samples (1,37,76) or in the differential detection of enzymes (73). Increases in our understanding of the microbial ecology of HMW PAH-degrading communities and the mechanisms by which HMW PAH biodegradation occur will prove helpful for predicting the environmental fate of these compounds and for developing practical PAH bioremediation strategies in the future.…”

Section: Hmw Pah Biodegradationmentioning

confidence: 99%

Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria

2000

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Section: Hmw Pah Biodegradationmentioning

confidence: 99%

Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria

2000

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“…If the biochemistry and genetic diversity are known, gene probe suites have greater potential for accurately evaluating bacterial degradative potential (234,424), although the application of a small number of probes may be effective if meaningful hypotheses are tested (565). Recent advances in characterizing alkane metabolism in a number of organisms have allowed the production of a variety of primers to detect, for example, the alkB gene from P. putida GPo1 (573).…”

Section: Culture-independent Approachesmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,172

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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“…Furthermore, Cercospora species are not known to be significant members of the microflora of the leaf surfaces of these hosts. However, Xanthomonas species have been identified that are capable of degrading diverse organic compounds that they may not commonly encounter in nature (1,23,34,35,41,45). Selected examples of aromatic molecules degraded by Xanthomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of the Polyketide Toxin Cercosporin

Mitchell¹,

Chilton

Daub

2002

Appl Environ Microbiol

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Cercosporin is a non-host-specific polyketide toxin produced by many species of plant pathogens belonging to the genus Cercospora. This red-pigmented, light-activated toxin is an important pathogenicity determinant for Cercospora species. In this study, we screened 244 bacterial isolates representing 12 different genera for the ability to degrade cercosporin. Cercosporin degradation was determined by screening for the presence of cleared zones surrounding colonies on cercosporin-containing culture medium and was confirmed by assaying the kinetics of degradation in liquid medium. Bacteria belonging to four different genera exhibited the cercosporin-degrading phenotype. The isolates with the greatest cercosporin-degrading activity belonged to Xanthomonas campestris pv. zinniae and X. campestris pv. pruni. Isolates of these pathovars removed over 90% of the cercosporin from culture medium within 48 h. Bacterial degradation of red cercosporin was accompanied by a shift in the color of the growth medium to brown and then green. The disappearance of cercosporin was accompanied by the appearance of a transient green product, designated xanosporic acid. Xanosporic acid and its more stable lactone derivative, xanosporolactone, are nontoxic to cercosporin-sensitive fungi and to plant tissue and are labile in the presence of light. Detailed spectroscopic analysis (to be reported in a separate publication) of xanosporolactone revealed that cercosporin loses one methoxyl group and gains one oxygen atom in the bacterial conversion. The resulting chromophore (4,9-dihydroxy-3-oxaperlylen-10H-10-one) has never been reported before but is biosynthetically plausible via oxygen insertion by a cytochrome P-450 enzyme.

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Detection of polycyclic aromatic hydrocarbon degradation genes in different soil bacteria by polymerase chain reaction and DNA hybridization

Cited by 85 publications

References 28 publications

Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria

Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria

Recent Advances in Petroleum Microbiology

Biodegradation of the Polyketide Toxin Cercosporin

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