Detection of Pneumocystis carinii, Mycobacterium tuberculosis, and Cytomegalovirus in Human Immunodeficiency Virus (HIV)-infected Patients with Hemophilia by Polymerase Chain Reaction of Induced Sputum Samples.

Shimomoto, Hiroshi; Hasegawa, Yoshinori; Takagi, Norio; Ichiyama, Satoshi; Takamatsu, Junki; Saito, Hidehiko; Shimokata, Kaoru

doi:10.2169/internalmedicine.34.976

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Cited by 4 publications

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“…Induced sputum has emerged as an important sampling technique for the assessment of lower airway secretions and is less invasive than bronchoscopy (Gibson et al, 2000). The application of molecular biological techniques has permitted rapid and more accurate diagnosis of infections caused by respiratory viruses (Turner, 1990) and other organisms (Sing et al, 2000;Mathis Am Weber et al, 1997;Leibovitz et al, 1995;Khan et al, 1999;Shimotmoto et al, 1995) in other settings.…”

Section: Introductionmentioning

confidence: 99%

Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques

Simpson

Moric

Wark

et al. 2003

Journal of Clinical Virology

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Section: Introductionmentioning

confidence: 99%

Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques

Simpson

Moric

Wark

et al. 2003

Journal of Clinical Virology

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Direct detection of Mycobacterium tuberculosis using polymerase chain reaction assay among patients with hepatic granuloma

Alcantara-Payawal

Matsumura

Shiratori

et al. 1997

Journal of Hepatology

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Induced Sputum: Selection Method

Efthimiadis²,

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Human Airway Inflammation

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Sputum consists of a mixture of mucus, inflammatory and epithelial cells, and cellular degradation products from the lower respiratory tract. In normal subjects, the inflammatory cells are mainly neutrophils and macrophages, with small numbers of lymphocytes, eosinophils, mast cells, and basophils. When expectorated the sputum is commonly contaminated with a variable amount of saliva, which has abundant squamous epithelial cells, and sometimes with secretions from the nasopharynx. These can be reduced by blowing the nose, rinsing the mouth, and swallowing water before expectoration. Contamination with saliva can be minimized by selecting the sputum from the expectorate and discarding the remainder. Selection of sputum (1) improves between-observer repeatability of differential cell counts (2,3) and allows standardization of measurements of total cell counts (expressed per gram of sputum) and fluid-phase mediators (by knowledge of the dilution factor of the processed sample).

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Detection of Pneumocystis carinii, Mycobacterium tuberculosis, and Cytomegalovirus in Human Immunodeficiency Virus (HIV)-infected Patients with Hemophilia by Polymerase Chain Reaction of Induced Sputum Samples.

Cited by 4 publications

References 21 publications

Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques

Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques

Direct detection of Mycobacterium tuberculosis using polymerase chain reaction assay among patients with hepatic granuloma

Induced Sputum: Selection Method

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