Detection of platelet microRNA expression in patients with diabetes mellitus with or without ischemic stroke

Duan, Xiaoling; Zhan, Qimin; Song, Bingxin; Zeng, Sian; Zhou, Juan; Long, Yuming; Lü, Jinfeng; Li, Zhibin; Yuan, Mei; Chen, Xiaoping; Yang, Qidong; Xia, Jian

doi:10.1016/j.jdiacomp.2014.04.012

Cited by 80 publications

(57 citation statements)

References 23 publications

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“…Among the 4 miRNAs (miR-107, miR-128b, miR-153 and miR-137) screened in the present study, circulating levels of miR-128b and miR-153 were the first time to be examined in ischemic stroke patients, whereas miR-107 and miR-137 were reported in similar studies before (17,18). In a study by Duan et al, they did not observe a significant change in the plasma level of miR-107 in ischemic stroke patients (18), whereas we did.…”

Section: Discussionsupporting

confidence: 55%

The Diagnostic Value of Circulating Brain-specific MicroRNAs for Ischemic Stroke

Yang

Zhang

et al. 2016

Intern. Med.

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Objective Circulating microRNAs have been recognized as promising biomarkers for various diseases. The aim of the present study was to explore the potential role of circulating miR-107, miR-128b and miR-153 as non-invasive biomarkers in the diagnosis of ischemia stroke. Methods One hundred and fourteen ischemic stroke patients (61±11.3 years old) and 58 healthy volunteers (56±3.9 years old) matched for age and sex were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. The circulating microRNAs levels were measured by quantitative real-time polymerase chain reaction. Results The circulating levels of miR-107, miR-128b and miR-153 significantly increased 2.78-, 2.13-and 1.83-fold in ischemia stroke patients in comparison to the healthy volunteers, respectively. Receiver operating characteristic (ROC) curves were analyzed using the SPSS software program and revealed the areas under the curve for circulating miR-107, miR-128b and miR-153 to be 0.97, 0.903 and 0.893 in ischemia stroke patients in comparison to healthy volunteers, respectively. The levels of circulating miR-107, miR-128b and miR-153 therefore positively correlated with the severity of stroke as defined by NIHSS classes. Conclusion Our results suggest that circulating miR-107, miR-128b and miR-153 might be used as potential novel non-invasive biomarkers for the diagnosis of ischemia stroke. However, future prospective trials in large-sized patient cohorts are needed before drawing any definitive conclusions.

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Section: Discussionsupporting

confidence: 55%

The Diagnostic Value of Circulating Brain-specific MicroRNAs for Ischemic Stroke

Yang

Zhang

et al. 2016

Intern. Med.

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“…miR-339 could be a pro-survival miRNA with neuroprotective effects in ischemic stroke models [17,18]. Low expression of miR-223 is a risk factor for ischemic stroke [19], which was negatively associated with National Institutes of Health Stroke Scale (NIHSS) scores and infarct volume [20], and its neuroprotective effect could be induced by glutamate receptors [21]. The involvement of miR-145 in the anti-proliferation and anti-inflammation events [22] inhibits the plaque progression in the cerebral vessels.…”

Section: The Potential Of Acupuncture For Secondary Prevention Of Strmentioning

confidence: 99%

Regulation of Hypertension for Secondary Prevention of Stroke: The Possible ‘Bridging Function' of Acupuncture

Zheng¹,

Han²,

Du³

et al. 2018

Complement Med Res

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Worldwide, stroke is the leading cause of mortality and disability, with hypertension being an independent risk factor for a secondary stroke. Acupuncture for the treatment of hypertension gains more attention in alternative and complementary medicine, but the results are inconsistent. Few studies regarding the secondary prevention of stroke by managing hypertension with acupuncture have been carried out as there are some problems regarding the antihypertensive drug status in the secondary prevention of stroke. Still, the potential of acupuncture in regulating the blood pressure for secondary stroke prevention deserves our focus. This review is based on papers recorded in the PubMed, Embase, and Web of Science databases, from their inception until March 28, 2017, and retrieved with the following search terms: hypertension and acupuncture, limited in spontaneously hypertensive rats (SHRs), stress-induced (or cold-induced) hypertensive or pre-hypertensive models. We find that, in these hypertensive animals, acupuncture could mainly influence factors related to the nervous system, oxidative stress, the endocrine system, cardiovascular function, and hemorheology, which are closely associated with the stroke outcome. This trend may give us a hint that acupuncture might well participate in the secondary prevention of stroke through these pathways when used in the management of hypertension.

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“…However, the expression of miR-144 in patients with ischemic stroke has not been reported. In contrast, circulating miR-223 levels has recently been demonstrated to be a risk factor for ischemic stroke in diabetes mellitus patients [18]. However, the targets of miR-223 have not been identified in patients with ischemic stroke.…”

Section: Introductionmentioning

confidence: 95%

Biomarkers Associated with Ischemic Stroke in Diabetes Mellitus Patients

et al. 2015

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Diabetes is an established risk factor for ischemic stroke, but the associated molecular mechanisms remain to be fully elucidated. This study investigated the role of plasma and platelet microRNAs and their targeting proteins in the activation of platelets and their association with the occurrence of ischemic stroke in patients with type 2 diabetes mellitus (T2DM). Results showed that the expressions of platelet and plasma miR-144 and miR-223 were significantly altered in T2DM patients with or without ischemic stroke compared to that in healthy controls, but these changes were more significant in T2DM patients with ischemic stroke. The expressions of P2Y12 and IRS-1 as well as phosphorylation levels of IRS-1, PI3K, and Akt in platelets were significantly altered in T2DM patients with or without ischemic stroke. The expression of platelet miR-144 and miR-223 significantly correlated with their plasma levels, P2Y12 and IRS-1 expression, blood glucose concentration, and platelet activation rate. High glucose concentration significantly elevated P-selectin, miR-144 and P2Y12 expression and significantly reduced miR-223 and IRS-1 expression in UT-7 cells. Overexpression of miR-223 and blocking of miR-144 expression significantly normalized the effects of high glucose concentration in UT-7 cells. In conclusion, hyperglycemia may activate platelets through miR-144 and miR-223 to downregulate IRS-1 and upregulate P2Y12 expression in the platelets of T2DM patients through an IRS-1-PI3K-Akt signaling. Low platelet and plasma miR-223 expression in addition to high platelet and plasma miR-144 expression are risk factors for ischemic stroke in T2DM patients.

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Detection of platelet microRNA expression in patients with diabetes mellitus with or without ischemic stroke

Cited by 80 publications

References 23 publications

The Diagnostic Value of Circulating Brain-specific MicroRNAs for Ischemic Stroke

The Diagnostic Value of Circulating Brain-specific MicroRNAs for Ischemic Stroke

Regulation of Hypertension for Secondary Prevention of Stroke: The Possible ‘Bridging Function' of Acupuncture

Biomarkers Associated with Ischemic Stroke in Diabetes Mellitus Patients

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