Detection of Platelet Antibodies by a Newly Developed
Mixed Agglutination with Platelets

Shibata, Yoichi; Juji, Takeo; Nishizawa, Yaeko; Sakamoto, Hisahiro; Ozawa, Naohiro

doi:10.1159/000460609

Cited by 60 publications

(73 citation statements)

References 8 publications

(8 reference statements)

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“…[11]. In brief, the platelets were isolated from the plateletrich plasma by centrifugation at l,300g for 15 min.…”

Section: Mixed Passive Hemagglutination Assaymentioning

confidence: 99%

Red Cells, HLA and Platelet Antibody Formation in Patients with Multiple Transfusions

Chow

Hu²,

Lyou³

et al. 1994

Acta Haematol

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Totally 436 Chinese patients having received multiple transfusions of red cells and platelets on more than three occasions were screened for red cell antibodies. Twenty-six (6%) of them were positive. Anti-E, -Mia, and -c were the common alloantibodies. Nine patients were immunized during the period of regular transfusions, with a newly immunized rate of 2% (9/436). Among 436 patients, 387 were screened for HLA antibodies by lymphocytotoxicity test (LCT). The overall positive rate was 35%. Most of the antibodies identified were against the high-frequency HLA antigens in the Chinese population. About 10% of the LCT-positive cases reverted to negative state during the follow-up period. After chloroquine stripping of the target platelets, mixed passive hemagglutination assay was used to detect platelet antibodies other than HLA antibody. Fifty-eight of 161 cases (36%) were positive for platelet antibodies, but half of them disappeared within 1 month. Nineteen of the 58 patients had sepsis and 2 had jaundice. These findings suggested that HLA and platelet antibodies are common among Chinese, though their clinical significance and the role in platelet damage are doubtful.

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“…[11]. In brief, the platelets were isolated from the plateletrich plasma by centrifugation at l,300g for 15 min.…”

Section: Mixed Passive Hemagglutination Assaymentioning

confidence: 99%

Red Cells, HLA and Platelet Antibody Formation in Patients with Multiple Transfusions

Chow

Hu²,

Lyou³

et al. 1994

Acta Haematol

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“…After the separation of macrophages, Tcells and B-cells were separated by Nylon-wool column [23]. Red cells and platelets were separated by centrifugation [25]. …”

Section: Immunologic Studiesmentioning

confidence: 99%

Effect of Preoperative Thoracic Duct Drainage on Canine Kidney Transplantation

Funakoshi

Carrieri

Yagihashi

et al. 1996

Journal of Investigative Surgery

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Chronic drainage of the thoracic duct to the esophagus was developed in dogs, and its efficacy in immunomodulation was tested using kidney transplantation. Compared to 9.7 days in the control, the mean animal survival was prolonged to 9.9 days, 17.8 days, and 18.5 days when TDD was applied preoperatively for 3 weeks, 6 weeks, and 9 weeks, respectively. Prolongation was significant after 6 weeks. Patency of the fistula was 93.5, 80.4, and 76.1% at respective weeks. Number of peripheral T-lymphocytes determined by a new monoclonal antibody diminished after 3 weeks. All animals were in normal health, requiring no special care for fluid, electrolyte, or protein replacement. Gowans' classical studies of thoracic duct drainage in rats in 1959 [7] focused attention on the lymphocyte as a specific target for selective immunosuppression of the immune system. Attempts to reduce the lymphocyte mass with excision of lymphoid organs (spleen and thymus), antilymphocyte sera (ALS) or globulins (ALG), and total lymphoid irradiation (TLI) can be traced directly back to Gowans' work, which showed that both cellular and humoral immunity were profoundly depressed in the rat within 5 days of TDD [8]. Woodruff and Anderson [9] demonstrated an additive or synergistic effect ofTDD and ALG.Attempts to apply TDD for clinical renal transplantation by Franksson [10] and others [11][12][13][14][15][16][17] were disappointing. Much later, it was shown in patients with autoimmune diseases [18] and in transplant recipients [19][20][21] that immunosuppression was not demonstrable until after 3 to 4 weeks of TDD instead of the 5 days in rats. Studies in large animal were hampered, as they were in humans, by the difficulty of maintaining long-term external TDD. Techniques in dogs for internal drainage of thoracic duct lymph into the esophagus also were unreliable. Canine studies were further limited by the inability to accurately monitor lymphocyte subpopulations. We have reexamined TDD in dogs, using a modification of an old technique that allows the reliable internal drainage of thoracic duct lymph into the esophagus for more than 9 weeks. Immunosuppression was tested with renal allotransplantation. In addition, we developed a new monoclonal antibody called IYF-l, which recognizes enriched canine T-Iymphocytes and allows changes to be followed in the lymphocytes of the thoracic duct lymph and peripheral blood.

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“…The Yuk phenotype was determined by the standard MPHA method. 17) Isolation of total cellular RNA and cDNA synthesis. Human platelets were obtained from anticoagulated whole blood by using differential centrifugation.…”

Section: Sequence Variation O F Human Platelet Membrane Glyco Proteinmentioning

confidence: 99%

Sequence Variation of Human Platelet Membrane Glycoprotein IIIa Associated with the Yuka/Yukb Alloantigen System.

Wang

Juji

Shibata

et al. 1991

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Detection of Platelet Antibodies by a Newly Developed Mixed Agglutination with Platelets

Cited by 60 publications

References 8 publications

Red Cells, HLA and Platelet Antibody Formation in Patients with Multiple Transfusions

Red Cells, HLA and Platelet Antibody Formation in Patients with Multiple Transfusions

Effect of Preoperative Thoracic Duct Drainage on Canine Kidney Transplantation

Sequence Variation of Human Platelet Membrane Glycoprotein IIIa Associated with the Yuka/Yukb Alloantigen System.

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