Detection of phenol by incorporation of gold modified-enzyme based graphene oxide thin film with surface plasmon resonance technique

Abstract

In this study, the incorporation between gold modified-tyrosinase (Tyr) enzyme based graphene oxide (GO) thin film with surface plasmon resonance (SPR) technique has been developed for the detection of phenol. SPR signal for the thin film contacted with phenol solution was monitored using SPR technique. From the SPR curve, sensitivity, full width at half maximum (FWHM), detection accuracy (DA) and signal-to-noise ratio (SNR) have been analyzed. The sensor produces a linear response for phenol up to 100 µM with sensitivity of 0.00193° µM−1. Next, it can be observed that deionized water has the lowest FWHM, with a value of 1.87° and also the highest value of DA. Besides, the SNR of the SPR signal was proportional to the phenol concentrations. Furthermore, the surface morphology of the modified thin film after exposed with phenol solution observed using atomic force microscopy showed a lot of sharp peaks compared to the image before in contact with phenol proved the interaction between the thin film and phenol.

Preparation of Molecularly Imprinted Optical Sensors for The Real Time Detection of Phenol

“…The limit of detection is calculated as 214 ppb. Hashim et al [50] prepared gold modified tyrosinase (Tyr) enzyme-based graphene oxide (GO) thin film on surface plasmon resonance (SPR) sensor surface for phenol detection. The thin film prepared was characterized by using atomic force microscopy (AFM).…”

Cellulose and Vanadium Plasmonic Sensor to Measure Ni2+ Ions

“…In addition to sensitivity and binding affinity parameters, full width at half maximum (FWHM) is also an important parameter, which the value should be as small as possible for a good sensor. A smaller value of FWHM implies a narrow and sharp reflectance curve, signifying a better detection accuracy of measurements [67][68][69]. The variation of FWHM with respect to Ni(II) ion concentrations is shown in Figure 6.…”

Recent Advances on Detection of Insecticides Using Optical Sensors

“…Surface plasmon resonance (SPR) strategy has drawn great attention as an economical, label-free tool because of its ability to detect target compounds in high sensitivity, real-time manner and rapid detection [ 42 , 43 , 44 , 45 , 46 , 47 , 48 ]. The Kretschmann configuration-based SPR method works by detecting the resonance in the form of charge density oscillations between the analyte and a metal thin film [ 49 , 50 , 51 , 52 , 53 , 54 , 55 ].…”