Detection of pharmaceutical compounds in tissue by matrix-assisted laser desorption/ionization and laser desorption/chemical ionization tandem mass spectrometry with a quadrupole ion trap

Abstract

Journal of the American Society for Mass Spectrometry 10 (1999) 1315-1321. doi:10.1016/S1044-0305(99)00103-82016-03-04T18:48:28

Desorption electrospray ionization mass spectrometry: Imaging drugs and metabolites in tissues

“…Whereas MALDI has been primarily used for profiling and imaging of peptides and proteins from tissues and cells, there is growing interest in applying MALDI to the analysis of small molecules, such as drugs and their metabolites (8)(9)(10)(11)(12)(13)(14)(15). The development of smallmolecule imaging by using MALDI has been challenging, primarily because matrix ions and mixed analyte-matrix clusters crowd the low-mass range, limiting confident detection of analyte ions of Ͻ750 Da.…”

Processing MALDI mass spectra to improve mass spectral direct tissue analysis

“…MALDI profiling and imaging has already been successfully applied to samples ranging from individually captured cells [11,12], to intact organs, and whole animal sagittal sections [1]. Although a relatively young method, tissue analysis by imaging mass spectrometry (IMS) has been used to address a number of clinical questions in cancer [13,14], neurodegenerative diseases [15][16][17], organ development [18] and the study of drug and metabolite distribution [19][20][21]. Due to the variety of applications and the potential of tissue analysis by MALDI mass spectrometry to offer solutions directed at difficult problems in diagnostic and prognostic medicine, tissue profiling and imaging will continue to be an important topic of research.…”

Matrix‐assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin‐fixed tissue

“…Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) [1] directly measures the in situ distribution of analytes such as drugs, [2,3] lipids, [4,5] peptides, [1,[6][7][8][9] and proteins. [1,[10][11][12] The advantages of the MALDI-IMS approach include the ability to analyze hundreds of analytes in a single experiment without prior knowledge of tissue composition, or the use of antibodies.…”