Detection of pea seed-borne mosaic virus virion and pinwheel inclusion body proteins in leaf and seed tissues of pea (Pisum sativum L) by immunogold labelling

Wang, D.; Woods, R. D.; Swaby, A. G.; Cockbain, A. J.

doi:10.1051/agro:19910506

Cited by 5 publications

(3 citation statements)

References 12 publications

(15 reference statements)

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“…This was confirmed by electron microscopy where typical PSbMV-associated structures, including pin-wheel inclusions similar to those seen in leaf mesophyll cells (Wang et al, 1991), were always found in sections of sepal, petal, anther-epidermal tissues and carpel. Virus particles were also observed, most frequently as bundles of particles (Wang et al, 1991). In contrast, none of these structures were seen inside pollen grains or ovules from either cultivar; funicle tissue adjacent to the ovule showed some cells containing PSbMV.…”

Section: Detection Of Psbmv In Floral Tissuessupporting

confidence: 58%

“…Sepal, petal, pollen, anther and carpel samples were collected from infected plants of Progreta and Vedette and assayed for PSbMV using indirect ELISA (Cockbain et al, 1988) or processed for electron microscopy (Wang et al, 1991). Pollen grains from single flowers were washed several times by mild sonication in extraction buffer (20 raM-sodium phosphatebuffered saline, pH 7.4, containing 0.05% v/v Tween 20, 2% polyvinyl pyrrolidone and 0.1% Triton X-100) and centrifugation, and finally ground by mortar and pestle to be used as individual samples in indirect ELISA.…”

Section: Methodsmentioning

confidence: 99%

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Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus

Wang

Maule

1992

Journal of General Virology

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Seed transmission of an isolate of pea seed-borne mosaic virus (PSbMV) in several pea genotypes has been studied. Cross-pollination experiments showed that pollen transmission of PSbMV did not occur and accordingly, virus was not detected in pollen grains by ELISA or electron microscopy. Comparative studies between two pea cultivars, one with a high incidence of seed transmission and one with none, showed that PSbMV infected the floral tissues (sepals, petals, anther and carpel) of both cultivars, but was not detected in ovules prior to fertilization. Virus was detected equally well in seed coats of the progeny in both cultivars. Analysis of virus incidence and concentration in pea seeds of different developmental stages demonstrated that in the cultivar with a high incidence of seed transmission, PSbMV directly invaded immature embryos, multiplied in the embryonic tissues and persisted during seed maturation. In contrast, the cultivar without seed transmission did not show invasion of immature embryos by the virus; there was no evidence for virus multiplication or persistence during embryo development and seed maturation. Hence seed transmission of PSbMV resulted from direct invasion of immature pea embryos by the virus and the block to seed transmission in the nonpermissive cultivar probably occurred at this step.

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Section: Detection Of Psbmv In Floral Tissuessupporting

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus

Wang

Maule

1992

Journal of General Virology

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“…PSbMV has filamentous particles and measures approximately 770 nm in length and 12 nm in width. It can induce the production of inclusion bodies that are pinwheel in shape and aggregate in the mesophyll cells (Hampton et al, 1981;Wang et al, 1991 andMakkouk et al, 1993). PSbMV can infect host range limited to the Fabaceae family such as pea (Pisum sativum), faba bean (Vicia faba), lentil (Lens culinaris), chickpea (Cicer arietinum), and pasture legumes such as alfalfa (Medicago sativa) and vetch (Vicia spp.)…”

Section: Introductionmentioning

confidence: 99%

Incidence, Distribution and Molecular Characterization of Pea Seed-borne Mosaic Virus (PSbMV) in Egypt

Farrag

Soliman²,

Al-Abhar³

et al. 2022

Egyptian Journal of Phytopathology

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Pea seed-borne mosaic virus is an important disease of pea crop and has a wide host range. The field survey was conducted in 2020 and 2021 in different locations of pea-growing fields of four governorates in Egypt to determine the incidence of the virus. Symptoms of virus infection were observed in 25.2 and 28.3 % of total surveyed fields at nine of ten regions in 2020 and 2021, respectively with an incidence ranged between 2 to 28.7%. Naturally infected pea leaves showed symptoms of severe mosaic, downward leaf rolling, vein banding, yellowing, and interveinal chlorosis. The isolated virus was identified based on the symptoms developed on diagnostic hosts, seed transmission, and molecular techniques. The seed transmission tests indicated that seed germination was affected by the virus, as it decreased the germination rate by 56%. The virus was transmitted through seeds where reverse transcription-polymerase chain reaction was used to detect the virus in seedlings produced from infected seeds and the percentage of seed transmission was 18%. 335 bp of the potyvirus coat protein gene and 800 bp of nuclear inclusion protein (NIb) gene of the virus were amplified using a set of degenerate and specific primers, respectively. The two sets of primers succeeded to amplify the partially coat protein gene of a potyvirus and a portion of the NIb gene of the virus. The amplified product was successfully cloned and sequenced. Results of sequence analysis showed similarity ranging between 95-100% compared with twelve reported isolates of the virus. The Egyptian isolate of Pea seed-borne mosaic virus was submitted in the GenBank under the accession number ON075784.

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Ultrastructural and Temporal Observations of the Potyvirus Cylindrical Inclusions (CIs) Show That the CI Protein Acts Transiently in Aiding Virus Movement

Roberts¹,

et al. 1998

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A systematic ultrastructural study across the edge of an advancing infection in pea seed-borne mosaic potyvirus-infected pea cotyledons showed the cylindrical inclusion (CI) protein to exist in transient functional states. Initially, the characteristic CI pinwheel inclusion bodies were positioned centrally over the plasmodesmal apertures (including those of plasmodesmata connected to the previously infected cell), in agreement with a proposed role in virus movement (Carrington et al., 1998, Plant J., 13, in press). The viral coat protein was associated with these structures and was seen within the modified plasmodesma, most notably in a continuous channel that passed along the axis of the pinwheel and through the plasmodesma. The CI protein was not detected within the plasmodesmal cavities. Later in the infection (i.e., behind the zone of active virus replication) the CI was no longer associated with cell walls, or with coat protein, and showed signs of structural degeneration. In contrast, the coat protein remained within plasmodesmal cavities. The role of the CI in assisting virus movement is not known but the presence of the CI was linked with an apparent transient reduction in callose in the vicinity of the plasmodesmata.

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Detection of pea seed-borne mosaic virus virion and pinwheel inclusion body proteins in leaf and seed tissues of pea (Pisum sativum L) by immunogold labelling

Abstract: Summary — An immunogold labelling procedure was developed to detect pea seed-borne mosaic virus (PSbMV)

Cited by 5 publications

References 12 publications

Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus

Early embryo invasion as a determinant in pea of the seed transmission of pea seed-borne mosaic virus

Incidence, Distribution and Molecular Characterization of Pea Seed-borne Mosaic Virus (PSbMV) in Egypt

Ultrastructural and Temporal Observations of the Potyvirus Cylindrical Inclusions (CIs) Show That the CI Protein Acts Transiently in Aiding Virus Movement

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