Detection of PCB77 by Antibody-Coated Competitive Fluorescent Quantitative Immuno-PCR Using Molecular Beacon

Abstract

A rapid and quantitative technique is urgently needed in detecting toxicological and carcinogenic polychlorinated biphenyls (PCBs) in the environment. In this study, a high sensitive real time fluorescent quantitative immuno-PCR (FQ-IPCR) approach using molecular beacon (MB) was developed to detect PCB77, which was classified as a human carcinogen. MB-based FQ-IPCR was then performed on serial dilutions of known PCB77 concentrations equivalent to 10-fold dilutions of 10-10 5 fg=mL. A correlation coefficient of 0.997 was identified, and a linear relationship between 10 fg=mL and 100 pg=mL, with y ¼ 0.543x þ 21.779, was obtained. Three soil samples were determined by MB-based FQ-IPCR to proof the validity of the method, and the results of which were further confirmed by conventional enzyme-linked immuno sorbent assay (ELISA). Based on sensitivity and reproduction, the MB-based real time 2774 FQ-IPCR technique will become a promising monitoring tool for environmental endocrine disruptors.

Detection of surface-linked polychlorinated biphenyls using surface-enhanced Raman scattering spectroscopy

“…Recent progress towards new PCB detection schemes include electrochemical impedance sensing using a single-walled carbon nanotube [12], electrochemical magneto-immunosensor [13], changes in surface photovoltage of porous ZnO [14], assay based on immuno-polymerase chain reaction [15], attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy [16], fluorescence [17,18] and surface plasmon resonances (SPRs) in thin gold films [19,20]. In terms of detection speed, ease of handling and reliability there is still room for better PCB sensors.…”

An indirect competitive enzyme-linked immunosorbent assay for the determination of 3,4-dichlorobiphenyl in sediment using a specific polyclonal antibody

“…Recently, there has been an increasing use of immunoassays for the detection of environmental contaminants because of their reliability, rapid detection, ease-of-operation, and relatively low cost. 15 During the past two decades, several immunoassays, including the radioimmunoassay, 16,17 ELISA, [18][19][20] the uoroimmunoassay, 21 immunosensor assay, [22][23][24] bioelectrochemical immunoassay, 25,26 real-time quantitative uorescence immuno PCR, [27][28][29] and commercial PCB test kits, have been developed for PCB detection in the environment. Immunoassays are also capable of detecting a wide variety of PCB congeners at submicrogram levels.…”

“…Indeed, a large number of studies using immunoassays for the determination of PCB concentrations (individual congeners or sums of various congeners) have been performed in the past few decades; however, most of the studies were focused on the "dioxin-like" PCBs, 21,[28][29][30][31][32] the indicator PCBs (PCB-28, 52, 101, 138, 153, 180 and occasionally PCB-118), 23 and the PCB mixture such as Aroclor. 19,25 Only a few studies focused on the detection of other single PCB congeners.…”