Detection of pathogenic<i>Escherichia coli</i>and microbiological quality of chilled shrimp sold in street markets

Barbosa, Liliana José; Ribeiro, Laryssa Freitas; Lavezzo, L. F.; Barbosa, Mayhara Martins Cordeiro; Rossi, Gabriel Augusto Marques; Amaral, Luiz Augusto do

doi:10.1111/lam.12562

Cited by 14 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In seafood, STEC prevalence of 1.3% to 4.6% has been registered in fish (Cardozo et al., 2018; Ribeiro et al., 2016) and up to 6% in shrimp (Dib et al., 2018). The presence of this pathogen in seafood could be related to the level of faecal environmental contamination (Ahmed et al., 2015), and the hygiene of handlers and their utensils (Barbosa et al., 2016). Here, we did not detect the presence of STEC in seafood, which could be related to good storage conditions, proper hygiene of handlers and utensils, and geographical location of suppliers, which are distant from most highly livestock areas located in the southern region of the country.…”

Section: Discussionmentioning

confidence: 99%

Genomic features and antimicrobial resistance patterns of Shiga toxin‐producing Escherichia coli strains isolated from food in Chile

Sánchez

Fuenzalida

Ramos

et al. 2021

Zoonoses and Public Health

View full text Add to dashboard Cite

Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that causes severe illness in humans, often associated with foodborne outbreaks. Antimicrobial resistance among foodborne E. coli has increased over the last decades becoming a public health issue. In this study, the presence and features of STEC were investigated in samples of meat, seafood, vegetables and ready-to-eat street-vended food collected in Chile, using a genomic and microbiological approach. Phenotypic and genotypic antimicrobial resistance profiles were determined, and serotype, phylogroup, sequence type (ST) and phylogenomics were predicted using bioinformatic tools. Three thousand three hundred samples collected in 2019 were screened, of which 18 were positive for STEC strains (0.5%), with stx2a (61.1%) being the predominant stx subtype. The presence of the virulence genes lpfA (100%), iha and ehaA (94.4%), and ehxA, hlyA and saa (83.3%) was confirmed among the STEC strains; the Locus of adhesion and autoaggregation (LAA) was predicted in 14 (77.8%) strains. Strains displayed resistance to colistin (100%), and intermediate resistance to enrofloxacin (11.1%) and chloramphenicol (5.6%). In this regard, mutations in the two-component regulatory system genes pmrA (S29G), pmrB (D283G) and phoP (I44L), and the presence of the qnrB19 gene were confirmed. STEC strains belonged to ST11231 (38.9%), ST297 and ST58 (16.7% each), and ST1635, ST11232, ST446, ST442 and ST54 (5.6% each), and the most frequently detected serotypes were O113:H21 (44.4%), O130:H11 and O116:H21 (16.7% each), and O174:H21 (11.1%). Strains belonging to the international ST58 showed genomic relatedness with worldwide strains from human and non-human sources. Our study reports for the first time the genomic profile of STEC strains isolated from food in Chile, highlighting the presence of international clones and sequence types commonly associated with human infections in different geographical regions, as well as the convergence of virulence and resistance in STEC lineages circulating in this country. | 227 SÁNCHEZ Et al.

show abstract

Section: Discussionmentioning

confidence: 99%

Genomic features and antimicrobial resistance patterns of Shiga toxin‐producing Escherichia coli strains isolated from food in Chile

Sánchez

Fuenzalida

Ramos

et al. 2021

Zoonoses and Public Health

View full text Add to dashboard Cite

show abstract

“…Based on the socio‐economic importance and the growing trend of street foods in Lebanon, the objective of this study was to assess the hygiene and sanitation in food stalls, by settling a correlation test between the analysis of food safety observational checklist and microbiological analyses. The microbiological quality of foods is a crucial parameter in terms of public health and it contributes in assessing the hygienic and sanitary conditions of the food production process (Barbosa et al, ). In this context, results of this study evaluate the microbiological quality of street foods served in Beirut city, highlight the potential causes related to several bad food practices in street food stalls and emphasize on possible improvements, the whole leading to safe food products.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, the raw meat contamination begins at the farm and might occur at any step of the food supply chain, to finally reside in the food preparation areas of food stalls (Sprenger, ). In addition, the availability of clean water for cleaning and hand washing plays an important role in decreasing the risk of contamination by E. coli , which is found in animals ‘intestines as well as in water contaminated with feces, and persists for a long period in the environment especially in crowded areas where no proper water supplies and drainage systems are available (Barbosa et al, ; Reddi, Kumar, Balakrishna, & Rao, ).…”

Section: Discussionmentioning

confidence: 99%

Street foods in Beirut city: An assessment of the food safety practices and of the microbiological quality

Loukieh

Mouannes

Jaoudeh

et al. 2018

Journal of Food Safety

View full text Add to dashboard Cite

Street food is a prevalent part of the food service industry. It is convenient in terms of accessibility and cost, but perceived as potential risk of foodborne illness. Accordingly, the safety of street foods in Beirut, Lebanon was assessed using an observational checklist and microbiological analyses. A total of 30 vendors were observed for their food safety parameters and 60 samples were analyzed for their content of Escherichia coli, Staphylococcus aureus, Listeria spp., and Salmonella spp., then, statistical analyses were performed for possible correlations. Results showed unsatisfactory levels of foodborne pathogens. Microbiological contamination was significantly correlated to unproper environmnental surroundings, deficiency of potable water, money handling, inappropriate methods of refrigeration, and the limited access to improper disposal facilities near stalls. These findings reveal the microbiological quality of street foods served in Beirut, and highlight on the practices to be ameliorated to provide safe street food products to consumers. Practical applicationsStreet food emerges as a social adaptation in urban areas due to its rapid lifestyle hence it may present a public health risk due to various factors. The current study is the first of its kind to be conducted in Beirut and its results present solid basis for further practices at different levels.Divulgation of its results would lead to new governmental laws to protect the public health and reduce the economic burdens related to hospitalization due to foodborne pathogens and poisoning. Educational institutions would also be involved in applications related to training and awareness sessions for street food handlers, to teach them the good hygienic practices. This study will also help consumers identify good and bad hygienic practices thus choose appropriate street food handlers.

show abstract

“…In situations where vendors do not perform the hand washing procedure during handling and marketing (handling of money, waste and utensils). The food handler can food (Barbosa et al, 2016;Loukieh et al, 2018). All kinds of food collected had at least one sample with the microbiological load above that allowed by the Brazilian legislation.…”

Section: Evaluation Of Good Practices For the Handling Of Street Vendingmentioning

confidence: 99%

Street food in Espírito Santo, Brazil: a study about good handling practices and food microbial quality

2021

View full text Add to dashboard Cite

Detection of pathogenicEscherichia coliand microbiological quality of chilled shrimp sold in street markets

Cited by 14 publications

References 26 publications

Genomic features and antimicrobial resistance patterns of Shiga toxin‐producing Escherichia coli strains isolated from food in Chile

Genomic features and antimicrobial resistance patterns of Shiga toxin‐producing Escherichia coli strains isolated from food in Chile

Street foods in Beirut city: An assessment of the food safety practices and of the microbiological quality

Street food in Espírito Santo, Brazil: a study about good handling practices and food microbial quality

Contact Info

Product

Resources

About