Detection of pathogenic Aeromonas hydrophilafrom two rainbow trout (Oncorhynchus mykiss) farms in Peru

Moya‐Salazar, Jeel; Díaz, Carmen Rosa; Cañari, Betsy; Badillo, Renato Xavier; Verano-Zelada, Mario; Chicoma-Flores, Karina; Contreras‐Pulache, Hans

doi:10.29374/2527-2179.bjvm000922

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…However, the long-term use and wide use of ceftazidime cause the development of drug resistance in clinical isolates (Sanz-García et al, 2018 ; Fröhlich et al, 2022 ). Not only in humans but also in untreated wastewater environments and aquaculture, ceftazidime-resistant bacteria are widespread (Adelowo et al, 2018 ; Salgueiro et al, 2021 ; Moya-Salazar et al, 2022 ), which poses a challenge to control infection caused by the resistant pathogens in hospitals and aquaculture. Recently, we have used gas chromatography-mass spectrometry (GC-MS) to profile the metabolic state of ceftazidime-resistant V. alginolyticus and identified an altered and inefficient pyruvate cycle (P cycle), increased biosynthesis of fatty acids, deregulation of Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR), and low membrane proton motive force (PMF) as the greatest metabolic modulation in the resistance (Liu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism

Xiang,

Wang,

Tao

et al. 2023

Front. Microbiol.

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IntroductionBacterial metabolic environment influences antibiotic killing efficacy. Thus, a full understanding for the metabolic resistance mechanisms is especially important to combat antibiotic-resistant bacteria.MethodsIsobaric tags for relative and absolute quantification-based proteomics approach was employed to compare proteomes between ceftazidime-resistant and -sensitive Edwarsiella tarda LTB4 (LTB4-RCAZ and LTB4-S, respectively).ResultsThis analysis suggested the possibility that the ceftazidime resistance mediated by depressed glucose is implemented through an inefficient metabolic flux from glycolysis, the pyruvate cycle, glutamate metabolism to purine metabolism. The inefficient flux was demonstrated by the reduced expression of genes and the decreased activity of enzymes in the four metabolic pathways. However, supplement upstream glucose and downstream guanosine separately restored ceftazidime killing, which not only supports the conclusion that the inefficient metabolic flux is responsible for the resistance, but also provides an effective approach to reverse the resistance. In addition, the present study showed that ceftazidime is bound to pts promoter in E. tarda.DiscussionOur study highlights the way in fully understanding metabolic resistance mechanisms and establishing metabolites-based metabolic reprogramming to combat antibiotic resistance.

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Section: Introductionmentioning

confidence: 99%

A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism

Xiang,

Wang,

Tao

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

Detection of pathogenic Aeromonas hydrophilafrom two rainbow trout (Oncorhynchus mykiss) farms in Peru

Abstract: H. (2022). Detection of pathogenic Aeromonas hydrophila from two rainbow trout (Oncorhynchus mykiss) farms in Peru.

Cited by 1 publication

References 14 publications

A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism

A glucose-mediated antibiotic resistance metabolic flux from glycolysis, the pyruvate cycle, and glutamate metabolism to purine metabolism

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