Detection of parvovirus B19 in synovial fluids of patients with osteoarthritis

Aslan, Bahadir; Serin, Mehmet Sami; Aslan, Gönül; Kalacı, Aydıner; Yanat, Ahmet Nedim; Tezcan, Seda; Emekdaş, Gürol

doi:10.1016/j.diagmicrobio.2007.11.003

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…Viral infection has been suggested as an etiological factor in OA, but unequivocal proof is lacking. While viral nucleic acids and antibodies have been demonstrated in blood and synovial fluids/tissues from OA patients, studies monitoring the presence of viral activity, either as transcripts, proteins, or viral particles, in cartilage or chondrocytes from such patients have not been reported, so that a causal role of viruses in this disease remains enigmatic [ 6 , 8 , 12 , 16 , 18 , 23 , 24 ]. The conclusion of a contributing role of viruses in OA has also been hampered by the absent or relatively few healthy, non-OA patients examined in comparative studies.…”

Section: Discussionmentioning

confidence: 99%

Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

Bendiksen

Martinez-Zubiavrra

Tümmler

et al. 2014

BioMed Research International

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The etiology of viruses in osteoarthritis remains controversial because the prevalence of viral nucleic acid sequences in peripheral blood or synovial fluid from osteoarthritis patients and that in healthy control subjects are similar. Until now the presence of virus has not been analyzed in cartilage. We screened cartilage and chondrocytes from advanced and non-/early osteoarthritis patients for parvovirus B19, herpes simplex virus-1, Epstein Barr virus, cytomegalovirus, human herpes virus-6, hepatitis C virus, and human endogenous retroviruses transcripts. Endogenous retroviruses transcripts, but none of the other viruses, were detected in 15 out the 17 patients. Sequencing identified the virus as HERV-WE1 and E2. HERV-W activity was confirmed by high expression levels of syncytin, dsRNA, virus budding, and the presence of virus-like particles in all advanced osteoarthritis cartilages examined. Low levels of HERV-WE1, but not E2 envelope RNA, were observed in 3 out of 8 non-/early osteoarthritis patients, while only 3 out of 7 chondrocytes cultures displayed low levels of syncytin, and just one was positive for virus-like particles. This study demonstrates for the first time activation of HERV-W in cartilage of osteoarthritis patients; however, a causative role for HERV-W in development or deterioration of the disease remains to be proven.

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Section: Discussionmentioning

confidence: 99%

Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

Bendiksen

Martinez-Zubiavrra

Tümmler

et al. 2014

BioMed Research International

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“…This study showed that Parvovirus B19 has a greater tendency to be a disease of acquired cause, with virus positivity already in late adolescence 12 and higher prevalence in older adults, especially women. ( 11 ),( 13 Rollín et al ( 10 ) reinforce the acquired cause theory of the disease after obtaining a similar prevalence (16.7% and 20%) in their case-control study performed with young mesenchymal cell transplantation in patients with osteoarthritis, showing the absence of significant virus reactivation after transplantation. However, they do not specify that there is no relationship and that it cannot also be of congenital cause.…”

Section: Discussionmentioning

confidence: 75%

Relationship Between Parvovirus B19 and Osteoarthritis: Literature Review

MARQUES,

DURIGON,

SARTI

et al. 2023

Acta ortop. bras.

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Osteoarthritis and Parvovirus B19 infection present silent and gradual evolution, since the former is a degenerative process while the latter is often asymptomatic and may persist in the individual’s body during their life. This study aims to analyze clinical studies that establish a correlation between degenerative osteoarthritis and Parvovirus B19 infection. Of the 62 studies found, 25 were chosen for reading in full. Analyzing only the studies that establish the correlation between the pathologies, seven confirm this relationship between Parvovirus B19 and Osteoarthritis, while one reports no relationship. No objective correlation could be found between the other articles studied. Our findings suggest that there is a close relationship between Parvovirus B19 and Osteoarthritis, with a higher prevalence of acquired causes, women and older adults, but it can manifest during life. However, it is essential to carry out new studies involving family history of patients with Osteoarthritis with positivity of Parvovirus B19, cohort studies between childhood and adult-old adult, so that it can elucidate this duality of congenital-acquired cause and, finally, raise treatment alternatives. Level of Evidence II, Systematic Review of Level II Studies.

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“…B19V DNA prevalence in synovial tissue of RA patients has been shown in several studies, including cohort studies investigating the presence of B19V infection markers (genomic sequences and virus-specific antibodies) in association with the level of cytokines and RA clinical activity and aggressiveness (Naciute et al, 2016). Very few studies have been reporting on the influence of B19V in OA patients (Rollín et al, 2007;Aslan et al, 2008). Difficulties arising around the excellent diagnosis of parvovirus-induced arthritis have been reported previously (Schmid et al, 2007), even in cases confirmed by the presence of parvovirus B19V DNA in synovial tissue.…”

Section: Discussionmentioning

confidence: 99%

“…However, one of the possible candidates contributing to the pathogenesis of OA could be a viral infection, which has been implicated in other inflammatory arthritides, like rheumatoid arthritis. Furthermore, it remains controversial whether human erythrovirus strains related to human parvovirus B19 provoke OA or worsen the clinical presentation of the disease (Aslan et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Synovitis in Osteoarthritic Patients: Morphological and Virological Evidence of its Contribution to Development of the Disease

Tarasovs

Skuja

Semenistaja

et al. 2019

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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The role of inflammation in the development of osteoarthritic joint degeneration is not completely understood. Recent data suggest that processes that cause and orchestrate inflamed synovial lesions may be implicated in the development of the disease. The morphological changes of the synovium in patients with osteoarthritis (OA), as well as the level of synovial inflammation cautiously graded, in association to the presence of human parvovirus B19 (B19V) infection markers, were evaluated. Qualitative and quantitative detection of B19V genomic sequence was performed in OA and rheumatoid arthritis (RA) groups. The expression of CD68, S100 (Ca2+ binding proteins soluble in 100% ammonium sulfate) and B19 VP1/VP2 capsid proteins found in the synovium were investigated by single and double immunolabeling, whereas fine features of synoviocytes — by electron microscopy. One-third of OA and RA patients demonstrated synovial expression of B19V antigen, which was confirmed in both types of synoviocytes. The overall expression of B19V in OA patients was weaker than that found in RA subjects. Positive correlation between B19V-positive vascular endothelial cells, sublining infiltrating lymphocytes, macrophages, and B19V-positive synoviocytes was established. No correlation between synovitis score indices as well as the expression of S100 and expression of B19V was found. The results suggest that the synovial membrane maintains local joint homeostasis, and that virus mediated synovitis is implicated in the development of OA.

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Detection of parvovirus B19 in synovial fluids of patients with osteoarthritis

Cited by 6 publications

References 25 publications

Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

Human Endogenous Retrovirus W Activity in Cartilage of Osteoarthritis Patients

Relationship Between Parvovirus B19 and Osteoarthritis: Literature Review

Synovitis in Osteoarthritic Patients: Morphological and Virological Evidence of its Contribution to Development of the Disease

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