Detection of paraoxon by continuous flow system based enzyme sensor

Jeanty, G.; Marty, Jean‐Louis

doi:10.1016/s0956-5663(97)00035-3

Cited by 74 publications

(30 citation statements)

References 25 publications

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“…In fact, in some cases the better LOD is characterized by a very limited linear range (see for example ref. [41]) or LOD reaches a very low value only when different definitions of this parameter are given (see ref. [42], whose results were not reported in the table due to many different LOD definitions given along the paper).…”

Section: Discussionmentioning

confidence: 99%

An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos

et al. 2011

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The characterization of an economic and ease-to-use carbon paste acetylcholinesterase (AChE) based biosensor to determine the concentration of pesticides Paraoxon and Dichlorvos is discussed. AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). When AChE is immobilized into a paste carbon working electrode kept at +410 mV vs. Ag/AgCl electrode, the enzyme reaction rate using acetylthiocholine chloride (ATCl) as substrate is monitored as a current intensity. Because Paraoxon and Dichlorvos inhibit the AChE reaction, the decrease of the current intensity, at fixed ATCl concentration, is a measure of their concentration. Linear calibration curves for Paraoxon and Dichlorvos determination have been obtained. The detection limits resulted to be 0.86 ppb and 4.2 ppb for Paraoxon and Dichlorvos, respectively, while the extension of the linear range was up 23 ppb for the former pesticide and up to 33 ppb for the latter. Because the inhibited enzyme can be reactivated when immediately treated with an oxime, the biosensor reactivation has been studied when 1,1 0 -trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were used. TMB-4 resulted more effective. The comparison with the behavior of similar AChE based biosensors is also presented.

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Section: Discussionmentioning

confidence: 99%

An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos

et al. 2011

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“…[9] This technique has used for OP insecticides monitoring by immobilizing AChE on a gold-coated nylon mesh by a self-assembled monolayer of cystamine preadsorbed on the gold surface [15] and a platinum electrode by entrapment in a photocrosslinker polymer [32] with the detection limits of 50 nm (defined as the concentration of inhibitor required to obtained a 5 % of inhibition) and in micromolar range, respectively. The inhibited AChE on the gold-coated nylon mesh could be reactivated by immersion in a solution of 2-pyridinealdoxime methiodide (2-PAM) for at least 6 h. [15] Here, a more sensitive, faster and cheap method for flow-injection detection of organophosphorous insecticides as shown in Figure 1 was developed with a detection limit of 1.0 nm at a 10 % inhibition for Sulfotep; the inhibited AChE could be regenerated for 15 cycles by using 2-PAM within 15 min.…”

Section: Introductionmentioning

confidence: 99%

Binding of Acetylcholinesterase to Multiwall Carbon Nanotube‐Cross‐Linked Chitosan Composite for Flow‐Injection Amperometric Detection of an Organophosphorous Insecticide

Kandimalla

2006

Chemistry A European J

109

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A novel method for immobilization of acetylcholinesterase (AChE) by binding covalently to a cross-linked chitosan-multiwall carbon nanotube (MWNT) composite is described. In addition a sensitive, fast, cheap and automatizable flow injection detection of an organophosphorous insecticide was developed. The MWNTs were homogeneously distributed in the chitosan membrane which showed a homogeneous porous structure. The immobilized AChE could catalyze the hydrolysis of acetylthiocholine with a K(M)app value of 177 microM to form thiocholine, which was then oxidized to produce detectable signal in a linear range of 1.0-500 microM and fast response. MWNTs could catalyze the electrooxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of an organophosphorous insecticide on the enzymatic activity of AChE, using Sulfotep as a model compound, the conditions for the flow-injection detection of the insecticide were optimized. Both biocompatibility of chitosan and inherent conductive properties of MWNTs favored the detection of the insecticide from 1.5 to 80 microM along with good stability and reproducibility. 95 % reactivation from inhibited AChE could be regenerated by using 2-pyridinealdoxime methiodide within 15 min for 15 times. The detection of Sulfotep samples exhibited satisfactory results. The proposed flow-injection analysis device can be applied to automated determination and characterization of enzyme inhibitors.

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“…Compared with the short lifetime in isolated thylakoid, 18,21 it is possible that the PVA-SbQ network is suitable for thylakoid immobilization, and the enzymes in a good microenvironment keep their activity for a long time. This stability is useful for prospective field measurements and to avoid repeated isolation procedures of thylakoid.…”

Section: Resultsmentioning

confidence: 99%

“…[19][20][21][22][23][24][25][26] Poly(vinylalcohol) (PVA) is a polymer that is frequently used as a matrix for the immobilization of various enzymes and cells. 27 The polymer uses the addition of styrylpyridinum groups attached to the poly(vinylalcohol) to cross-link the polymer chains under mild conditions without damaging the biological material to be entrapped.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Herbicide Biosensors Based on the Inhibition of Enzyme Activity That Catalyzes the Scavenging of Hydrogen Peroxide in a Thylakoid Membrane

Wei

Tian

2005

ANAL. SCI.

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Detection of paraoxon by continuous flow system based enzyme sensor

Cited by 74 publications

References 25 publications

An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos

An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos

Binding of Acetylcholinesterase to Multiwall Carbon Nanotube‐Cross‐Linked Chitosan Composite for Flow‐Injection Amperometric Detection of an Organophosphorous Insecticide

Fabrication of Herbicide Biosensors Based on the Inhibition of Enzyme Activity That Catalyzes the Scavenging of Hydrogen Peroxide in a Thylakoid Membrane

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