Detection of Paraneuronal Cells in Guppy (Poecilia vivipara) Gill Filament epithelium Determined by Histochemistry and Transmission Electron Microscopy

Oliveira, Rodinelli Borges de; Rosa, Eliane Vieira; Nascimento, Valdirene do; Menezes-Faria, Joana Cristina Neves; Világio, Ana Flávia; Morais, José Oscar Rodrigues; Cardoso, Márcia Pereira; Breseghelo, Leandro; Menezes, Janaína de Sousa; Vieira, Pabline Marinho; Sabóia, Simone Maria Teixeira de

doi:10.5216/rbn.v3i2.2622

Cited by 2 publications

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References 24 publications

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“…In the gill filaments of C. paleatus , neuroepithelial cells were found. These cells were identified because their morphology was very similar to those found in other teleosts, such as Oncorhynchus mykiss (Walbaum, 1792), Stizoztedion lucioperca (Linnaeus, 1758), Ameiurus melas (Rafinesque, 1820) (= Ictalurus melas ), Anguilla anguilla (Linnaeus, 1758) (Dunel‐Erb et al ., 1982), Hypostomus plecostomus (Linnaeus, 1758) (Fernandes & Perna‐Martins, 2001), Coelorhinchus caelorhinchus (Risso, 1810) (= Coelorhynchus coelorhynchus ) (Calabró et al ., 2005) and Poecilia vivipara Bloch & Schneider, 1801 (Borges de Oliveira et al ., 2006). These cells probably fulfil the role of oxygen sensors and contribute to regulate blood flow (Sudin & Nilsson, 2002).…”

Section: Discussionmentioning

confidence: 99%

Gill morphology and morphometry of the facultative air‐breathing armoured catfish, Corydoras paleatus, in relation on aquatic respiration

Plaul

Díaz

Barbeito

2021

Journal of Fish Biology

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The Neotropical armoured catfish Corydoras paleatus is a facultative air‐breathing teleost commonly exported as ornamental fish. In this species, air breathing enables it to survive and inhabit freshwater environments with low oxygen levels. Therefore, it is important to analyse the gills from a morphological aspect and its dimensions in relation to body mass with reference to aquatic respiration. For that, the gills were analysed using a stereoscopic microscope for morphometric studies, and structural and ultrastructural studies were carried out to compare the four branchial arches. Furthermore, two immunohistochemical techniques were used to locate and identify the presence of a Na+/K+ pump. The characterization of the potential for cell proliferation of this organ was assessed using an anti‐PCNA antibody. The results show that gills of C. paleatus present some characteristics related to its diet and lifestyle, such as the limited development of gill rakers and the abundance of taste buds. In addition, other special features associated with the environment and bimodal breathing were observed: scarce and absent mucous cells (MCs) in the gill filaments and branchial lamellae, respectively, and the localization of mitochondria‐rich cells (MRCs) covering the basal third of the branchial lamellae, which reduces the gill respiratory area. A peculiar finding in the gill epithelium of this armoured catfish was the presence of mononuclear cells with sarcomeres similar to myoid cells, whose functional importance should be determined in future studies. Finally, in C. paleatus, the interlamellar space of gill filaments is an important site for cell turnover and ionoregulation; the latter function is also performed by the branchial lamellae.

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Section: Discussionmentioning

confidence: 99%

Gill morphology and morphometry of the facultative air‐breathing armoured catfish, Corydoras paleatus, in relation on aquatic respiration

Plaul

Díaz

Barbeito

2021

Journal of Fish Biology

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Cellular proliferation in the gills of guppies exposed to pequi ethanolic extracts - Proliferação celular nas brânquias de guppies expostos a extratos etanólicos do pequi

et al. 2015

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The bioactive principles present in the ethanolic extract of "pequi" (Caryocar brasiliensis) have proven to be molluscicidal, fungicidal, trypanocidal, insecticide, and leshimanicidal, taking potential use to combat aquatic pests and vectors in public health, and for infectious and invasive diseases in fish. Many plants have proven moluscidida and insecticide effects, and its extracts may contribute to vector control in public health. They also have prophylactic and therapeutic effects in combating diseases in fish, for ornamental and aquaculture systems. Most of them are commonly found in tropical and subtropical countries. However, to be released in environment or used for health and/or combating invasive diseases in fish, the effect of these products on other representatives of aquatic fauna must be better understood. The guppy (Poecilia vivipara) is generally used as a bioindicator of environmental toxicity, then, he was elected to the experimental studies carried out to verify the action of these extracts. The gills are the target organ, as they come into direct contact with water and because its morphophysiological activity in demonstrate the effects of substances present in the water. Therefore, the extracts obtained from the stem and leaf of pequi were analyzed chromatographically, and then the extracts rich in flavonoids were selected. The effects in acute tests of these extracts were verified histologically and immunohistochemically using the molecular marker BrdU (5-bromo-2'-deoxyuridine) that binds to cellular DNA during the S phase of their cycle. This work confirms the potential action of "pequi" as inducer of cell proliferation in gills of fish. As the test was conducted in a short period of time and was proven the occurrence of cell proliferation exist the possibility of having future tumor formation, which indicate a need for studies with long-term exposures.

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Detection of Paraneuronal Cells in Guppy (Poecilia vivipara) Gill Filament epithelium Determined by Histochemistry and Transmission Electron Microscopy

Cited by 2 publications

References 24 publications

Gill morphology and morphometry of the facultative air‐breathing armoured catfish, Corydoras paleatus, in relation on aquatic respiration

Gill morphology and morphometry of the facultative air‐breathing armoured catfish, Corydoras paleatus, in relation on aquatic respiration

Cellular proliferation in the gills of guppies exposed to pequi ethanolic extracts - Proliferação celular nas brânquias de guppies expostos a extratos etanólicos do pequi

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