Detection of Paragonimus heterotremus Eggs in Experimentally Infected Cats by a Polymerase Chain Reaction–Based Method

Intapan, Pewpan M.; Wongkham, Chaisiri; Imtawil, Kanokwan; Pumidonming, Wilawan; Prasongdee, Thidarat K.; Misawa, Miwa; Maleewong, Wanchai

doi:10.1645/ge-3357rm

Cited by 25 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[24][25][26] However, relatively few studies have attempted to detect Paragonimus DNA in the definitive host. [27][28][29] Our PCR studies confirmed the presence of P. kellicotti DNA in clinical specimens from two Missouri cases. Both patients had clinically compatible illnesses after ingestion of raw crayfish.…”

Section: Discussionsupporting

confidence: 66%

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Fischer¹,

Curtis²,

Marcos³

et al. 2011

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Human paragonimiasis is an emerging disease in Missouri. To characterize local parasites, we examined crayfish from three rivers. Metacercaeriae consistent with Paragonimus kellicotti were detected in 69%, 67%, and 37% of crayfish from the Big Piney, Huzzah, and Black Rivers, respectively. Sequencing of the second internal transcribed spacer and other DNA markers confirmed the species identification and the presence of identical parasite sequences in clinical specimens from two human cases. Mongolian gerbils were infected by intraperitoneal injection with 3–8 metacercariae. Most gerbils died 15–49 days post-infection. Necropsies showed pulmonary hemorrhage with necrosis, and flukes as long as 8 mm were recovered from intrathoracic tissues. Western blot analysis using P. kellicotti antigen showed a strong antibody response in gerbils 39 days post-infection. These results demonstrate that P. kellicotti is common in Missouri crayfish. The gerbil model may be useful for research on the pathogenesis, immunology, and treatment of paragonimiasis.

show abstract

Section: Discussionsupporting

confidence: 66%

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Fischer¹,

Curtis²,

Marcos³

et al. 2011

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…This approach should be evaluated further using more faecal samples and highly specific and sensitive primers (Intapan et al, 2005). Serology, the copro-DNA test and detection of eggs in sputum (or faeces) are essential for differentiation of paragonimiasis from other diseases.…”

Section: Discussionmentioning

confidence: 99%

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa

Okamoto

Mabou³

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

View full text Add to dashboard Cite

SummaryParagonimiasis is a common parasitic zoonosis in Cameroon and neighboring countries in Western Africa. Serum, sputum and faecal samples were collected in an endemic area of South West Province, Cameroon, after administration of a questionnaire to identify individuals with appropriate symptoms and histories. Microscopic examination revealed eggs in sputum from 16 people, but none in any faecal sample. These 16 were among the 25 and 26 people respectively positive by enzyme-linked immunosorbent assay (ELISA) and by immunoblot using Paragonimus africanus crude antigens. The detection of copro-DNA by polymerase chain reaction (PCR) was attempted using 23 faecal samples (18 from sputum egg-negative and 5 from sputum egg-positive individuals). The copro-DNA was detected in 4 of the 5 sputum eggpositive individuals. These results suggest that (1) serology is much more sensitive than sputum examination for diagnosis of paragonimiasis, and (2) a copro-DNA test may be more sensitive than a microscopic search for eggs in faeces. Molecular sequence data from ITS2 and cox1 genes confirmed that adult worms experimentally raised in cats were P. africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis.

show abstract

“…Molecular assays, using PCR, have been developed for Opisthorchis viverrini (Wongratanacheewin et al 2002), Paragonimus spp. (Intapan et al 2005) and C. sinensis (Parvathi et al 2007) that offer improved sensitivity. Advances in recombinant protein expression are facilitating the development of ELISA-based detection methods.…”

Section: Zoonotic Trematode Infections (A) Fasciolosismentioning

confidence: 99%

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Robinson

Dalton

2009

Phil. Trans. R. Soc. B

148

109

View full text Add to dashboard Cite

Zoonotic infections are among the most common on earth and are responsible for .60 per cent of all human infectious diseases. Some of the most important and well-known human zoonoses are caused by worm or helminth parasites, including species of nematodes (trichinellosis), cestodes (cysticercosis, echinococcosis) and trematodes (schistosomiasis). However, along with social, epidemiological and environmental changes, together with improvements in our ability to diagnose helminth infections, several neglected parasite species are now fast-becoming recognized as important zoonotic diseases of humans, e.g. anasakiasis, several fish-borne trematodiasis and fasciolosis. In the present review, we discuss the current disease status of these primary helminth zoonotic infections with particular emphasis on their diagnosis and control. Advances in molecular biology, proteomics and the release of helminth genome-sequencing project data are revolutionizing parasitology research. The use of these powerful experimental approaches, and their potential benefits to helminth biology are also discussed in relation to the future control of helminth infections of animals and humans.

show abstract

Detection of Paragonimus heterotremus Eggs in Experimentally Infected Cats by a Polymerase Chain Reaction–Based Method

Cited by 25 publications

References 17 publications

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Contact Info

Product

Resources

About