Detection of Panton-Valentine Leukocidin-Positive Methicillin-Resistant<i>Staphylococcus aureus</i>Nasal Carriage among Egyptian Health Care Workers

Hefzy, Enas; Hassan, Gamal M.; Reheem, Fadwa Abd El

doi:10.1089/sur.2015.192

Cited by 22 publications

(23 citation statements)

References 36 publications

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“…Nasal colonization with S. aureus plays an essential role in transmission of infections [2,4]. Of particular importance are the nasal carriers of MRSA among medical staff who possess a higher risk for transmission of nosocomial infections [39][40][41]. MRSA is regarded as the most serious infection in hospitals [42].…”

Section: Discussionmentioning

confidence: 99%

Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students

Al‐Akchar

Himsawi

Abu-Raideh

et al. 2018

J Infect Dev Ctries

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Introduction: Nasal carriers of methicillin-resistant Staphylococcus aureus (MRSA) are common and play an important role in nosocomial infections. The prevalence rate and characterization of nasal carriers of MRSA among medical students in Jordan has not been investigated before. Methodology: The resistance of S. aureus to several antibiotics was tested using disc diffusion method, automatic Vitek 2, and penicillin binding protein (PBP) 2 slide test. Bacterial species and resistance genes were confirmed using molecular analysis of three relevant genes by real-time PCR. Two hundred ninety nasal swabs were collected from medical students at Hashemite University from June 2015 to August 2016. All participants signed a voluntary consent form and filled a predesigned questionnaire. Results: The mean age of participants was 19.7 ± 2 years and 61.7% of them were males. 63 out of the 290 (21.7%) samples were identified to have S. aureus, 56 (19.3%) were methicillin-sensitive S. aureus (MSSA) and 7 (2.4%) were MRSA. S. aureus nasal colonization significantly associates with male gender (OR = 1.7, CI = 0.94-3.18, P = 0.049) and chronic illnesses (OR = 4.0, CI = 1.52-10.65, P = 0.006). Consistency between disc diffusion, Vitek 2, and PBP 2 methods for MRSA screening were satisfactory compared to molecular analysis. All MRSA samples were positive for SCCmec:orfx junction gene (MRSA-specific), nuc gene (S. aureus- specific), mecA gene (PBP-mediated resistant), and PBP2 production. All MRSA isolates were multi-drug resistant and were sensitive to Linezolid, Vancomycin, and Tigecycline. Conclusions: This study confirms that nasal colonization by MRSA among medical students necessitates further attention to prevent nosocomial infections.

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Section: Discussionmentioning

confidence: 99%

Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students

Al‐Akchar

Himsawi

Abu-Raideh

et al. 2018

J Infect Dev Ctries

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“…In any case, the mecC -MRSA human prevalence detected in most of the studies was very low. Several studies did not identify any mecC -positive S. aureus among included human isolates/samples ( Table 2 ) [ 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. In studies in which this gene was detected ( Table 1 ), the prevalence identified, considering the total number of isolates/samples included, was < 1% in most of the cases [ 24 , 27 , 28 , 29 , 32 , 33 , 37 , 40 , 41 , 42 , 43 ], similar to that identified in the first study in which mecC was discovered (approximately 0.04%) [ 16 ].…”

Section: Detection Of Mecc -Mrsa Isolates In Humentioning

confidence: 99%

Human mecC-Carrying MRSA: Clinical Implications and Risk Factors

et al. 2020

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A new methicillin resistance gene, named mecC, was first described in 2011 in both humans and animals. Since then, this gene has been detected in different production and free-living animals and as an agent causing infections in some humans. The possible impact that these isolates can have in clinical settings remains unknown. The current available information about mecC-carrying methicillin resistant S. aureus (MRSA) isolates obtained from human samples was analyzed in order to establish its possible clinical implications as well as to determine the infection types associated with this resistance mechanism, the characteristics of these mecC-carrying isolates, their possible relation with animals and the presence of other risk factors. Until now, most human mecC-MRSA infections have been reported in Europe and mecC-MRSA isolates have been identified belonging to a small number of clonal complexes. Although the prevalence of mecC-MRSA human infections is very low and isolates usually contain few resistance (except for beta-lactams) and virulence genes, first isolates harboring important virulence genes or that are resistant to non-beta lactams have already been described. Moreover, severe and even fatal human infection cases have been detected. mecC-carrying MRSA should be taken into consideration in hospital, veterinary and food safety laboratories and in prevention strategies in order to avoid possible emerging health problems.

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“…Panton Valentine leukocidin (PVL), is encoded by lukF-PV and lukS-PV genes, produced by some S. aureus strains, is one of the significant cytotoxins and responsible for dermal and soft tissue infections (Genestier et al, 2005). Limited data available about the epidemiology of Egyptian PVL -positive MRSA strains (Helal et al, 2012 andHefzy et al, 2016) The present study aimed to assess the relatedness of MRSA isolates and the potential routes of transmission using PCR-RFLP by examining environmental samples and HCWs nasal swabs in relation to MRSA recovered from patients of Hemodialysis Unit of El Zagazig General Hospital, Sharqia, Egypt. In addition, the presence of PVL staphylococcal virulence factor gene was investigated.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Antimicrobial Susceptibility of Mrsa Isolated From Chronic Hemodialysis Outpatients and Their Correlation to Mrsa Colonization Among Healthcare Workers

Helal

Mohamed²,

El-Mahallawy³

et al. 2019

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The carriage of methicillin-resistant Staphylococcus aureus (MRSA) among dialysis patients is remarkable not only in terms of the risks of developing infections, but also in playing a principle part in transmission among dialysis unit staff. The aim of this study was to detect the colonization of Methicillin-sensitive Staphylococcus aureus and MRSA carriage. Also, our aim was to determine the relatedness of MRSA isolates and the potential routes of transmission using PCR- Restriction Fragment Length Polymorphism (PCR-RFLP) in Hemodialysis Unit of El Zagazig General Hospital, a tertiary medical center in Sharqia, Egypt. This study was conducted on 150 chronic hemodialysis outpatients and 200 non clinical control samples including environmental and healthcare workers (HCWs). Antibiotic susceptibility by VITEK-2 and disc diffusion, PCR amplification of mecA, pvl and coa genes and RFLP-PCR were conducted during the study period. In this study 3.3% of the patients and 3.2% of HCWs colonized with pvl positive MRSA. Fifty percent of MRSA isolates showed a single band PCR product amplification of 810bp fragment corresponding to coa gene. Ten distinct MRSA RFLP banding patterns designated as H1-H10 were obtained. The majority of strains belonged to RFLP banding pattern H1 (33.33%).The prevalence of MRSA carriage among hemodialysis patients was 14% and 9.7 % among HCWs with similar polymorphism patterns. The presence of one major coa gene type confirmed the occurrence of hospital acquired-associated MRSA.

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Detection of Panton-Valentine Leukocidin-Positive Methicillin-ResistantStaphylococcus aureusNasal Carriage among Egyptian Health Care Workers

Cited by 22 publications

References 36 publications

Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students

Nasal colonization by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among medical students

Human mecC-Carrying MRSA: Clinical Implications and Risk Factors

Molecular Characterization and Antimicrobial Susceptibility of Mrsa Isolated From Chronic Hemodialysis Outpatients and Their Correlation to Mrsa Colonization Among Healthcare Workers

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