Detection of Organophosphorus Pesticides Using Potentiometric Enzymatic Membrane Biosensor Based on Methylcellulose Immobilization

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

“…1. In this case the enzymatic activity can be measured by means of different electrochemical transducers: i) potentiometric: the reaction can be monitored by the measurement of the pH variation using a pH electrode [61], ISFETs [62,63], and electrodes modified with polymers [64][65][66]. A BChE based light addressable potentiometric sensor was developed by Mourzina et al [67].…”

Monolithic bioreactors: Effect of chymotrypsin immobilization on its biocatalytic properties

“…The immobilized enzymes are widely used in biotechnology [1], medical diagnostics [2] and therapy [3], biosensors [4], proteomics [5], etc. Solid phase attachment of enzymes has a number of advantages comparing to application of soluble biocatalysts.…”

Recent advances in biosensors based on enzyme inhibition

“…When recording the pH variation, the AChE activity was measured by potentiometric or optical transducers. To this regard, classical enzyme-modified pH electrodes (Zhang et al, 2009a) or miniaturized pH-sensitive field effect transistors were used. For instance, pH-FETs was used as transducer with immobilized BChE for the detection of solanaceous glycoalkaloids in the concentration range of 0.1 µM to 0.1 mM (Benilova et al, 2006).…”