Detection of Organophosphorus Compounds by Covalently Immobilized Organophosphorus Hydrolase

Orbulescu, Jhony; Constantine, Celeste A.; Rastogi, Vipin K.; Shah, Saumil S.; DeFrank, Joseph; Leblanc, Roger M.

doi:10.1021/ac061118m

Cited by 43 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Orbulescu et al. further demonstrated that covalently immobilizing fluorescently labeled OPH on a silanized quartz substrate results in an increase of the enzyme stability, while a detection limit in the n M concentration regime can be maintained 17…”

Section: Enzyme‐based Sensorsmentioning

confidence: 99%

Experimental Measurement of Carbohydrate–Aromatic Stacking in Water by Using a Dangling‐Ended DNA Model System

Morales¹,

Reina²,

Dı́az³

et al. 2008

Chemistry A European J

View full text Add to dashboard Cite

Protein-carbohydrate recognition is of fundamental importance for a large number of biological processes; carbohydrate-aromatic stacking is a widespread, but poorly understood, structural motif in this recognition. We describe, for the first time, the measurement of carbohydrate-aromatic interactions from their contribution to the stability of a dangling-ended DNA model system. We observe clear differences in the energetics of the interactions of several monosaccharides with a benzene moiety depending on the number of hydroxy groups, the stereochemistry, and the presence of a methyl group in the pyranose ring. A fucose-benzene pair is the most stabilizing of the studied series (-0.4 Kcal mol(-1)) and this interaction can be placed in the same range as other more studied interactions with aromatic residues of proteins, such as Phe-Phe, Phe-Met, or Phe-His. The noncovalent forces involved seem to be dispersion forces and nonconventional hydrogen bonds, whereas hydrophobic effects do not seem to drive the interaction.

show abstract

Section: Enzyme‐based Sensorsmentioning

confidence: 99%

Experimental Measurement of Carbohydrate–Aromatic Stacking in Water by Using a Dangling‐Ended DNA Model System

Morales¹,

Reina²,

Dı́az³

et al. 2008

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…ATCC 27551 and B. diminuta MG. It uses Co 2+ , Zn 2+ , Mg 2+ , Ca 2+ , and Fe 2+ for nucleophilic attack, thus hydrolyzing P-O, P-CN, P-F, and S bonds ( Ghanem and Raushel, 2005 ; Orbulescu et al, 2006 ). MPH was isolated from Plesiomonas sp.…”

Section: Molecular Mechanism Of Diazinon Biodegradationmentioning

confidence: 99%

Environmental Occurrence, Toxicity Concerns, and Degradation of Diazinon Using a Microbial System

Zhou

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Diazinon is an organophosphorus pesticide widely used to control cabbage insects, cotton aphids and underground pests. The continuous application of diazinon in agricultural activities has caused both ecological risk and biological hazards in the environment. Diazinon can be degraded via physical and chemical methods such as photocatalysis, adsorption and advanced oxidation. The microbial degradation of diazinon is found to be more effective than physicochemical methods for its complete clean-up from contaminated soil and water environments. The microbial strains belonging to Ochrobactrum sp., Stenotrophomonas sp., Lactobacillus brevis, Serratia marcescens, Aspergillus niger, Rhodotorula glutinis, and Rhodotorula rubra were found to be very promising for the ecofriendly removal of diazinon. The degradation pathways of diazinon and the fate of several metabolites were investigated. In addition, a variety of diazinon-degrading enzymes, such as hydrolase, acid phosphatase, laccase, cytochrome P450, and flavin monooxygenase were also discovered to play a crucial role in the biodegradation of diazinon. However, many unanswered questions still exist regarding the environmental fate and degradation mechanisms of this pesticide. The catalytic mechanisms responsible for enzymatic degradation remain unexplained, and ecotechnological techniques need to be applied to gain a comprehensive understanding of these issues. Hence, this review article provides in-depth information about the impact and toxicity of diazinon in living systems and discusses the developed ecotechnological remedial methods used for the effective biodegradation of diazinon in a contaminated environment.

show abstract

“…Orbulescu et al further demonstrated that covalently immobilizing fluorescently labeled OPH on a silanized quartz substrate results in an increase of the enzyme stability, while a detection limit in the nm concentration regime can be maintained. [17] Another method to access biosensors that incorporate OPH in combination with a fluorescent dye was developed by Russell et al [18] In this study OPH was covalently functionalized with the pH-dependent fluorescent label carboxy seminaphthofluorescein (SNAFL-1, Scheme 2c) and with a poly(ethylene glycol) (PEG) acrylate derivative. The acrylated fluorescent enzyme was photopolymerized in the presence of PEG diacrylate, trimethylolpropane triacrylate, and/ or tetraacrylated PEG to yield microspheres of a sensor-containing, lightly cross-linked polymer that formed hydrogels upon immersion in water.…”

Section: Enzyme-based Sensorsmentioning

confidence: 99%

Fluorescent Sensors for the Detection of Chemical Warfare Agents

Burnworth

Rowan

Weder

2007

Chemistry A European J

252

130

View full text Add to dashboard Cite

Along with biological and nuclear threats, chemical warfare agents are some of the most feared weapons of mass destruction. Compared to nuclear weapons they are relatively easy to access and deploy, which makes them in some aspects a greater threat to national and global security. A particularly hazardous class of chemical warfare agents are the nerve agents. Their rapid and severe effects on human health originate in their ability to block the function of acetylcholinesterase, an enzyme that is vital to the central nervous system. This article outlines recent activities regarding the development of molecular sensors that can visualize the presence of nerve agents (and related pesticides) through changes of their fluorescence properties. Three different sensing principles are discussed: enzyme-based sensors, chemically reactive sensors, and supramolecular sensors. Typical examples are presented for each class and different fluorescent sensors for the detection of chemical warfare agents are summarized and compared.

show abstract

Detection of Organophosphorus Compounds by Covalently Immobilized Organophosphorus Hydrolase

Cited by 43 publications

References 45 publications

Experimental Measurement of Carbohydrate–Aromatic Stacking in Water by Using a Dangling‐Ended DNA Model System

Experimental Measurement of Carbohydrate–Aromatic Stacking in Water by Using a Dangling‐Ended DNA Model System

Environmental Occurrence, Toxicity Concerns, and Degradation of Diazinon Using a Microbial System

Fluorescent Sensors for the Detection of Chemical Warfare Agents

Contact Info

Product

Resources

About