Detection of oral bacteria on the tongue dorsum using PCR amplification of 16S ribosomal RNA and its association with systemic disease in middle-aged and elderly patients

Su, Cheng-Yih; Shigeishi, Hideo; Nishimura, Rumi; Ohta, Kouji; Sugiyama, Masaru

doi:10.3892/br.2018.1175

Cited by 14 publications

(20 citation statements)

References 30 publications

(37 reference statements)

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“…Importantly, we found a significant negative correlation between the degree of oral moisture and the number of oral bacteria in older people. Sue et al have also described a correlation between the level of moisture and the number of bacteria on the tongue surface in middle-aged and older people [8]. Therefore, we speculate that dry mouth due to aging leads to an elevated number of oral bacteria.…”

Section: Discussionsupporting

confidence: 50%

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High Rate of Oral Candida Detection in Dependent Japanese Older People

Miyata

Shigeishi

et al. 2020

Geriatrics

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The aim of this study was to clarify the association between oral Candida detection and the dependency status of older people. This study included 31 older people aged ≥70 years who had a certified need for long-term care or support and received care in a local day care center; it also included 27 independent older patients aged ≥70 years who visited Hiroshima University Hospital. Oral Candida was detected by a polymerase chain reaction using swab samples from the tongue surface. Oral moisture was significantly reduced in dependent older people, compared with independent older people (p = 0.003). There was a weak negative relationship between numbers of bacteria and levels of oral moisture (Spearman’s rank correlation coefficient; R = −0.29, p = 0.01). Dependent older people exhibited a significantly higher rate of Candida albicans detection (35.5%) compared with independent older people (11.1%). Dependent older people also exhibited a higher rate of concurrent detection of both C. albicans and Candida glabrata (32.3%) compared with independent older people (11.1%), but this difference was not statistically significant. Thus, dependent older people may be more susceptible to oral C. albicans infection, compared with independent older people. Good oral hygiene is necessary to prevent oral Candida infection in dependent older people.

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Section: Discussionsupporting

confidence: 50%

“…An Orcellex ® Brush (Rovers Medical Devices, NL, Netherlands) was used to collect samples from the tongue surface, in accordance with the method used in a prior study [8]. The tongue surface was swabbed softly 10 times, using an Orcellex ® Brush.…”

Section: Oral Sample Processing and Dna Extractionmentioning

confidence: 99%

High Rate of Oral Candida Detection in Dependent Japanese Older People

Miyata

Shigeishi

et al. 2020

Geriatrics

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“…These conserved sequences can be used to design universal primers for bacterial DNA amplification. [ 10 ]…”

Section: Discussionmentioning

confidence: 99%

“…T. denticola has been associated with an increase in periodontal destruction, therefore, increasing tooth loss. [ 10 ] It is very difficult to isolate and identify T. denticola from clinical samples using culture technique, therefore, the 16S rRNA-based PCR method is used for determining their presence. [ 26 ]…”

Section: Discussionmentioning

confidence: 99%

Detection of Red complex bacteria, P. gingivalis, T. denticola and T. forsythia in infected root canals and their association with clinical signs and symptoms

Tiwari

Saxena

Kumari

et al. 2020

J Family Med Prim Care

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Aim: This study aimed to investigate the association between endodontic clinical signs and symptoms and the presence of Porphyromonas gingivalis, Treponema denticola , and Tannerella forsythia employing polymerase chain reaction (PCR). Materials and Methods: Microbial samples were obtained from 60 cases with necrotic pulp with primary teeth infections. DNA extracted from samples were analyzed for endodontic pathogens by using species-specific primers. Results: P. gingivalis/T. denticola were detected in 15 symptomatic teeth associated with periapical lesions. T. forsythia/T. denticola were found in 16 symptomatic teeth associated with pain and swelling. P. gingivalis was detected in 9 teeth which were associated with pain, 2 with tenderness on percussion, and 15 with periapical lesions. Statistically significant associations were found between T. forsythia as well as T. denticola in relation to clinical findings of pain and swelling. ( P < 0.05). Red complex bacteria showed no statistical significant association with the presence of signs and symptoms. Conclusion: Prevalence of P. gingivalis , T. denticola , and T. forsythia suggested association of these bacteria with symptomatic infected pulp and periradicular diseases.

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“…Although highly specific, the culture method requires approximately 5-7 days to positivity, but has low specificity [6], and is poorly suited for molecular epidemiological studies. PCR-based techniques, especially quantitative (qPCR) have been widely used to quantify P. gingivalis DNA in saliva, dental plaque and other samples [7]. Most of these assays require DNA extraction or release processes which are not only time-consuming, but also result in loss of template DNA [8].…”

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confidence: 99%

An evaluation of direct PCR assays for the detection and quantification of Porphyromonas gingivalis

Kong

et al. 2020

Epidemiol. Infect.

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Porphyromonas gingivalis has been linked to the development and progression of oesophageal squamous cell carcinoma (ESCC), and is considered to be a high-risk factor for ESCC. Currently, the commonly used methods for P. gingivalis detection are culture or DNA extraction-based, which are either time and labour intensive especially for high-throughput applications. We aimed to establish and evaluate a rapid and sensitive direct quantitative polymerase chain reaction (qPCR) protocol for the detection of P. gingivalis without DNA extraction which is suitable for large-scale epidemiological studies. Paired gingival swab samples from 192 subjects undergoing general medical examinations were analysed using two direct and one extraction-based qPCR assays for P. gingivalis. Tris-EDTA buffer-based direct qPCR (TE-direct qPCR), lysis-based direct qPCR (lysis-direct qPCR) and DNA extraction-based qPCR (kit-qPCR) were used, respectively, in 192, 132 and 60 of these samples for quantification of P. gingivalis. The sensitivity and specificity of TE-direct qPCR was 95.24% and 100% compared with lysis-direct qPCR, which was 100% and 97.30% when compared with kit-qPCR; TE-direct qPCR had an almost perfect agreement with lysis-direct qPCR (κ = 0.954) and kit-qPCR (κ = 0.965). Moreover, the assay time used for TE-direct qPCR was 1.5 h. In conclusion, the TE-direct qPCR assay is a simple and efficient method for the quantification of oral P. gingivalis and showed high sensitivity and specificity compared with routine qPCR.

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Detection of oral bacteria on the tongue dorsum using PCR amplification of 16S ribosomal RNA and its association with systemic disease in middle-aged and elderly patients

Cited by 14 publications

References 30 publications

High Rate of Oral Candida Detection in Dependent Japanese Older People

High Rate of Oral Candida Detection in Dependent Japanese Older People

Detection of Red complex bacteria, P. gingivalis, T. denticola and T. forsythia in infected root canals and their association with clinical signs and symptoms

An evaluation of direct PCR assays for the detection and quantification of Porphyromonas gingivalis

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