Detection of ochratoxin A in beer samples with a label-free monolithically integrated optoelectronic biosensor

Pagkali, Varvara; Petrou, Panagiota; Salapatas, Alexandros; Makarona, Eleni; Peters, Jeroen; Haasnoot, W.; Jobst, G.; Economou, Anastasios; Μισιακός, Κωνσταντίνος; Raptis, Ioannis; Kakabakos, Sotirios

doi:10.1016/j.jhazmat.2016.03.019

Cited by 46 publications

(31 citation statements)

References 43 publications

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“…This weaker attachment of the aptamer to the surface favors the aptamer–OTA interaction, the displacement of the aptamer, and cargo delivery. However, both LOD values comply with those established by the legislation and are similar to or better than most published techniques for the detection of OTA (see Table S4) …”

Section: Resultssupporting

confidence: 83%

“…This weaker attachment of the aptamer to the surfacef avors the aptamer-OTAi nteraction, the displacement of the aptamer,a nd cargo delivery.H owever,b oth LOD values comply with those established by the legislation and are similar to or better than most published techniques for the detection of OTA( see Ta ble S4). [42][43][44][45][46][47][48][49][50][51][52][53][54] Additionally,t he selectivity of S3 (Figure 3i nb lack) and S5 ( Figure 3i ng rey) to OTAw as also studied. With this aim, cargo releasefrom S3 and S5 was testedi np resence of OTA, fumonisin B1 and aflatoxin B1.…”

Section: Sensitivity and Selectivity Studiesmentioning

confidence: 99%

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Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer‐capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine B‐loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5–0.05 nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples.

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Section: Resultssupporting

confidence: 83%

Section: Sensitivity and Selectivity Studiesmentioning

confidence: 99%

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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“…Immunosensors using enzymatic labels (e.g., HRP, AP) tend to be sensitive because of the potential for significant amplification provided by the enzyme. They also tend to use commonly available optical or electrochemical readers that are relatively inexpensive [32][33][34][35][36]. The downside to these immunoassays is that most require additional steps (e.g., washing, adding substrate and/or mediator) that are avoidable with certain other formats.…”

Section: Discussionmentioning

confidence: 99%

“…An optical immunosensor for label-free detection of OTA in beer samples has been proposed by Pagkali et al [36]. The biosensor consisted of an array of ten monolithically integrated Mach-Zehnder interferometers (MZIs) along with their respective broad-band silicon light sources on the same Si chip.…”

Section: Biosensors For the Detection Of Ochratoxin A In Wine Beer Amentioning

confidence: 99%

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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Mycotoxins and haloanisoles are secondary metabolites produced under special conditions of temperature and humidity by fungi colonizing a variety of commodities from preharvest up to consumer use. Ochratoxin A and 2,4,6-trichloanisole are produced mainly by species of the genus Aspergillus and Penicillium. Ochratoxin A exhibits nephrotic effects and can, potentially, be associated with human carcinogenesis, whereas 2,4,6-trichloanisole is primarily responsible for cork taint in wines. This review provides an overview of recent advances in biosensor technology for the determination of the aforementioned compounds in wine, beer and other beverages, as well as cork stoppers, which help in establishing and carrying out proper product quality-management strategies. Such a detailed investigation of biosensor-based detection methods of these toxic compounds in beverages could lead to the provision of safe-to-consume products, and allow the prioritization of future research efforts.

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“…Aptamers, due to their excellent selectivity and large robustness , are exceptional recognition elements for fabricating biosensors. Several designs of aptamers‐based biosensors (aptasensors) have been proposed to detect OTA within the range of 0.173 pM–17 nM . In most of these studies low LODs have been achieved mainly by the use of amplifying elements like nanoparticles, proteins and enzymes .…”

Section: Introductionmentioning

confidence: 99%

Simple and Fast Picomolar Detection of Ochratoxin A Using a Reusable Label Free Aptasensor Built with a Layer‐by‐layer Procedure

et al. 2017

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A label free impedimetric aptasensor for simple, fast and reusable picomolar detections of Ochratoxin A (OTA) in grape juices was designed. Two main factors were observed to affect the accurate detections of the toxin: i‐lateral interactions between self‐assembled aptamers ii‐ adsorption of large molecules present in complex matrixes like grape juices. Lateral interactions between aptamers were minimized by constructing the aptasensor in a Layer‐by‐Layer procedure. The interferences associated to the unspecific and irreversible adsorption of large molecules present in grape juice, were reduced by submitting samples to ultrafiltration prior to analysis. With this protocol, a 0.12 pM limit of detection and 0.24 pM limit of quantification in spiked grape juices were achieved after only 5–7 mins of interaction with the samples. The presented aptasensor can be recovered after a simple immersion in hot water (90 °C) for ten minutes.

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Detection of ochratoxin A in beer samples with a label-free monolithically integrated optoelectronic biosensor

Cited by 46 publications

References 43 publications

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Simple and Fast Picomolar Detection of Ochratoxin A Using a Reusable Label Free Aptasensor Built with a Layer‐by‐layer Procedure

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