Detection of ochratoxin A by fluorescence sensing based on mesoporous materials

Abstract

We developed a new ochratoxin A (OTA) aptamer biosensor to promptly detect OTA in food. Mesoporous silica nanoparticles (MSN) were used as carriers, and aptamers were used as recognition probes and gating molecules. The fluorescent dye Rhodamine 6G (Rh6G) was loaded into mesoporous silica, and through electrostatic contact, the OTA aptamer was adsorbed on amino-modified mesoporous silica. The fluorescent dye released from the mesopore in the presence of OTA because of the conformational change induced in the aptamer by the target. The amount of ochratoxin was determined by measuring the fluorescence intensity. Our findings revealed a positive relationship between the fluorescence intensity and OTA concentration, with a limit of detection of 0.28 ng mL−1, and the detection range was 0.05–200 ng mL−1. The recovery rate was 80.7%–110.8% in real samples. The proposed approach is suitable for the quantification of other toxins.