Detection of occult hepatitis C virus among healthy spouses of patients with HCV infection

Shazly, Yahia El; Hemida, Khaled; Rafik, Mona; Swaff, Reham Al; Ali-Eldin, Zainab A.; GadAllah, Shaimaa

doi:10.1002/jmv.24074

Cited by 16 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, a recent study was conducted at Ain Shams University, Egypt, to identify OCI with genotype‐4 among healthy Egyptian sexual partners of patients with chronic HCV‐4 infection using real‐time PCR detection of HCV‐RNA in PBMCs. The prevalence of occult HCV‐4 infection in these partners was 4% [Shazly et al, ]. This rate is similar to ours but with a different selection criteria.…”

Section: Discussionsupporting

confidence: 85%

Occult hepatitis C virus infection among Egyptian hemodialysis patients

et al. 2016

View full text Add to dashboard Cite

Occult hepatitis C virus (HCV) infection (OCI) was reported in an apparently disease-free state in the absence of liver disease, anti-HCV and HCV-RNA in the serum. The existing data examining the clinical significance of OCI and its potential as a source of HCV infection among hemodialysis patients are very limited. We examined the presence of OCI among patients on maintenance hemodialysis at Minia Governorate, Egypt; an HCV endemic country. A total of 81 subjects with negative markers for HCV were enrolled. HCV-RNA was tested in PBMCs by real-time PCR. For the 81 subjects, the average dialysis duration was 32.7 ± 21.7 months and the average ALT level (±SD) was 26 ± 12 U/L while that of AST was 29 ± 16 U/L. Out of the 81 subjects, three (3.7%) were HCV-RNA positive in PBMCs in the absence of serum anti-HCV and HCV-RNA indicating OCI. The viral load of the OCI subjects ranged from 172 to 4150 IU/ml. History of liver disease was positive in one of the three positive patients. These results highlight the potential risk of HCV transmission from patients within hemodialysis units in Egypt. J. Med. Virol. 88:1388-1393, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Section: Discussionsupporting

confidence: 85%

Occult hepatitis C virus infection among Egyptian hemodialysis patients

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Detection hepatitis C virus RNA in plasma in this study (1.4%), this reveal false negative among negative result for hepatitis C virus Antibodies which done already in blood bank laboratory, because of error technique or blood donors in serocoversion period before appearance antibodies, so the true detection of OCI in our study was nine out of 140 (6.4%). This result closed to other investigation of Shazly et al (2015) [21] who documented that OCI was 4% in healthy Egyptian individual partners of patients infected with chronic HEPATITIS C VIRUS genotype 4 infection. Also in hemodialysis patients Abdurrahman et al (2016) [22] detect OCI (3.7%) in 81 at Mania-Egypt, Also Maria de la Luz Martinez-Rodríguez 2018 [23] in Mexico City documented OCI 3.4% in blood donors Samples, Lin H 2016 documented OCI 2.2%in China [24] and Quinoa JA 2016 OCI is 2.1% Spain [25].…”

Section: Discussioncontrasting

confidence: 48%

Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt

Abdeldayem¹

2019

AJLM

View full text Add to dashboard Cite

Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%-20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven't any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.

show abstract

“…We determined a strong association between homosexual relationships and OCI (OR = 5.52), which suggests that a homosexual relationship is a risk factor. Shazly et al reported that 4% of healthy spouses of CHC patients have OCI, supporting the notion that sexual transmission of OCI occurs [ 27 ]. In the present study, 97.4% were heterosexual and 3.2% of these were positive for OCI, which is in agreement with what was previously reported.…”

Section: Discussionmentioning

confidence: 81%

Prevalence and risk factors of Occult Hepatitis C infections in blood donors from Mexico City

et al. 2018

View full text Add to dashboard Cite

BackgroundThe circulatory system is the main mechanism for transmission of the Hepatitis C Virus (HCV). A new class of HCV infections, Occult HCV infection (OCI), is defined as the presence of HCV-RNA in hepatocytes with the absence of HCV in the serum/plasma utilizing current laboratory assays. Different groups have reported the prevalence of OCI; however, its associated risk factors have not been established. In Mexico, there are no reports about OCI, so the objective of our study was to determine the prevalence of OCI in total blood donors in Mexico City, as well as its associated risk factors.MethodsBlood donors that were considered eligible for donation, according to NOM 253-SSA1-2012, were randomly selected. Demographic data was collected from 1,037 donors. Plasma and peripheral blood mononuclear cells were assessed for HCV-RNA. The presence of HCV-RNA was determined by nested PCR for the 5'-UTR region. Logistic regression was used to calculate the odds ratio (OR) and 95% confidence interval (95%CI) to determine the level of association.ResultsThe prevalence of OCI was 3.4% among blood donors. Homosexual relationships (OR = 5.52, 95%CI: 1.53–19.92, p<0.05) and acupuncture (OR = 3.56, 95%CI: 1.41–8.98, p<0.05) were significantly associated with OCI.ConclusionThere is a significant presence of OCI in the blood donor population in Mexico City. The main risk factors for OCI transmission are homosexual relationships and acupuncture. This study supports the increased use of sensitive and specific screening tests for blood bank testing.

show abstract

Detection of occult hepatitis C virus among healthy spouses of patients with HCV infection

Cited by 16 publications

References 17 publications

Occult hepatitis C virus infection among Egyptian hemodialysis patients

Occult hepatitis C virus infection among Egyptian hemodialysis patients

Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt

Prevalence and risk factors of Occult Hepatitis C infections in blood donors from Mexico City

Contact Info

Product

Resources

About