Detection of Occult Hepatitis C and Hepatitis B Virus Infections from Peripheral Blood Mononuclear Cells

Abstract

Patients with long-standing abnormal results of liver-function tests with unknown etiology may have HCV RNA or HBV DNA in their PBMCs in the absence of anti-HCV antibodies, HBV markers, serum HBV DNA and serum HCV RNA.In Patients with SVR, HCV RNA in PBMCs is recommended to detect residual infection especially in those with high serum HCV RNA levels before treatment.

Occult Hepatitis B Virus Infection

“…Aynı zamanda OHB'nin, hemodiyaliz hastalarında da HBV bulaşı açısından bir risk faktörü olabileceği düşünülmektedir [17]. OHB, nedeni bilinmeyen serum ALT yüksekliği olan hastalarda tespit edilmiştir [16,46]. Ayrıca OHB'nin kriptojenik karaciğer hastalığı ilişkili olduğu veya HBsAg klirensinden sonra devam eden orta derecede karaciğer hasarının bu hastalarda dü-şük seviyede tespit edilebilen HBV DNA ile ilişkili olduğunu dü-şündüren çalışmalar da mevcuttur [47,48].…”

How to Diagnosis the Occult Hepatitis C Virus?

“…Amplification of HCV RNA in samples and internal control RNA is measured independently at different wavelengths due to probe labeling with different fluorescence reporter dyes (HCV RNA; FAM, Internal control RNA; Yakima Yellow). The detection limit of this assay was found to be 3 synthetic HCV RNAs per PCR run (1).…”

“…Future works should deal with the possible incidence of OHC or B infections and their pathologic and infective relevance specially in drug abusers, healthcare workers, patients who had received multiple blood transfusions, and patients on hemodialysis, etc. to know the prevalence and spread of this infection in these populations (1,7). Financial Disclosure: The authors declared that this study has received no financial support.…”

“…Different studies have revealed that HCV RNA can persist and replicate in immune cells but the relevance of its presence and persistence over time is still unknown. As the contribution of this extrahepatic reservoir could have several clinical implications in viral transmission, treatment response and disease pathogenesis, future studies are required to improve our knowledge of the extrahepatic manifestations of HCV infection and its possible consequences (1).…”

Occult hepatitis B virus infection in Egyptian hemodialysis patients with or without hepatitis C virus infection

“…However, the availability of highly sensitive polymerase chain reaction (PCR)-based techniques has allowed the identification of HBV DNA in hepatitis B surface antigen-negative individuals, with or without circulating antibodies to hepatitis B surface antigen and/or hepatitis B core antigen. 2 Recently, a group of patients has been identified as having occult HBV infection defined by positive HBV DNA in the liver or blood, while hepatitis B surface antigen is undetectable in serum as measured submit your manuscript | www.dovepress.com…”