Detection of Noroviruses in Ready-To-Eat Foods by Using Carbohydrate-Coated Magnetic Beads

Morton, Vanessa; Jean, Julie; Farber, Jeffrey M.; Mattison, Kirsten

doi:10.1128/aem.00202-09

Cited by 48 publications

(23 citation statements)

References 24 publications

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“…Cationic bead capture is an attractive concept as it may allow the detection of a wide range of viruses without pre-clearing samples. Recently, porcine mucin or synthetic histo-blood group antigens (HBGAs) have been coupled to magnetic beads for the concentration of NoV from food and environmental samples (Cannon and Vinje 2008;Morton et al 2009;Tian et al 2008). HBGAs have been proposed as the norovirus receptor and they have been proven to bind multiple strains of norovirus in vitro (Morton et al 2009;Tan and Jiang 2007).…”

Section: Sample Preparationmentioning

confidence: 99%

“…Recently, porcine mucin or synthetic histo-blood group antigens (HBGAs) have been coupled to magnetic beads for the concentration of NoV from food and environmental samples (Cannon and Vinje 2008;Morton et al 2009;Tian et al 2008). HBGAs have been proposed as the norovirus receptor and they have been proven to bind multiple strains of norovirus in vitro (Morton et al 2009;Tan and Jiang 2007). While magnetic beads are not a universal solution, they have the advantage of being easily separated from a number of food types, which eliminates upstream processing steps and saves time.…”

Section: Sample Preparationmentioning

confidence: 99%

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Analytical Methods for Food and Environmental Viruses

Mattison

Bidawid

2009

Food Environ Virol

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There are many challenges for developing and selecting methods to detect enteric viruses from food and environmental samples. Growth methods are rarely available and the viruses have a low infectious dose, so methods must be very sensitive as well as specific. This review discusses methods for sample preparation, detection and typing, outlining strengths and weaknesses for different protocols. Enteric viruses are very stable in the environment and the development of effective detection methods is an important step towards reducing contamination of foods and the environment.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Analytical Methods for Food and Environmental Viruses

Mattison

Bidawid

2009

Food Environ Virol

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“…Elution, using basic buffer or chemical treatment are often used before concentration step based on filtration or precipitation (Table 5). (Morton et al, 2009) …”

Section: Methods Of Detection For Viruses In Foodsmentioning

confidence: 99%

Scientific Opinion on an update on the present knowledge on the occurrence and control of foodborne viruses

2011

EFS2

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A review of the biology, epidemiology, diagnosis and public health importance of foodborne viruses was performed. Data needs to support a risk assessment were also identified. In addition possible control options and their anticipated impact to prevent or reduce the number of foodborne viral human infections were identified, including the scientific reasons for and against the establishment of food safety criteria and process hygiene criteria for viruses for certain food categories. Food may be contaminated by virus during all stages of the food supply chain, and transmission can occur by consumption of food contaminated during the production process (primary production, or during further processing), or contaminated by infected food handlers. Transmission of zoonotic viruses (e.g. HEV) can also occur by consumption of products of animal origin. Viruses do not multiply in foods, but may persist for extended periods of time as infectious particles in the environment, or in foods. At the EU-level it is unknown how much viral disease can be attributed to foodborne spread. The relative contribution of different sources (shellfish, fresh produce, food handler including asymptomatic shedders, food handling environment) to foodborne illness has not been determined. The Panel recommends focusing controls on preventive measures to avoid viral contamination rather than trying to remove/inactivate these viruses from food. Also, it is recommended to introduce a microbiological criteria for viruses in bivalve molluscs, unless they are labelled "to be cooked before consumption". The criteria could be used by food business operators to validate their control options. Furthermore, it is recommended to refine the regulatory standards and monitoring approaches in order to improve public health protection. Introduction of virus microbiological criteria for classification of bivalve molluscs production areas should be considered. A virus monitoring programme for compliance with these criteria should be risk based according to the findings of a sanitary survey. KEY WORDSFood borne viruses, Norovirus, Hepatitis, Microbiological criteria, molluscs, fresh produce. SUMMARYThe European Food Safety Authority (EFSA) asked the Panel on Biological Hazards to initiate a self tasking issue with the purpose to provide up-to-date information on the present knowledge on the occurrence and control of foodborne viruses. The BIOHAZ Panel carried out a review of the available information in the scientific literature with regards to the biology, epidemiology, diagnosis and public health importance of foodborne viruses. Where possible the review covered primary production, food harvesting, food processing, and storage/retail until consumption. Data needs to support a risk assessment were also identified. In addition possible control options and their anticipated impact to prevent or reduce the number of foodborne viral human infections were identified including the scientific reasons for and against the establishment of microbiological criteri...

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“…Histo-blood group antigens (HBGAs) have been characterized recently as receptors for HuNoV, and receptor-based capture assays have been applied to concentrate HuNoV from spiked food samples and spiked concentrated environmental water samples (2,21,33). Two-log-unit increases in the sensitivity of HuNoV detection in spiked food samples by a similar method have been reported (33).…”

mentioning

confidence: 98%

“…Several studies demonstrated that a HBGA receptor-based capture method could be used to concentrate HuNoV from food samples (21,33) and facilitate the removal of PCR inhibitors from concentrated water samples (2). Recently, Huhti et al (8) reported using an HBGA-binding assay together with electron microscropy (EM) to confirm morphological integrity, antigenicity, and functionality of HuNoV GII-4 viral like particles.…”

mentioning

confidence: 99%

Application of a Receptor-Binding Capture Quantitative Reverse Transcription-PCR Assay To Concentrate Human Norovirus from Sewage and To Study the Distribution and Stability of the Virus

Tian

Yang

Pan

et al. 2012

Appl Environ Microbiol

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Water is an important route for human norovirus (HuNoV) transmission. Using magnetic beads conjugated with blood grouplike antigens (HuNoV receptors), we developed a simple and rapid receptor-binding capture and magnetic sequestration (RBCMS) method and compared it to the existing negatively charged membrane absorption/elution (NCMAE) method for concentrating HuNoV from sewage effluent. RBCMS required 6-fold-less sample volume than the NCMAE method and also resulted in a significantly higher yield of HuNoV No GI and <2% GII HuNoV were detected in sewage samples stored at room temperature for 4 weeks. We conclude that RBCMS requires less sample volume, has better recovery and sensitivity, and is faster than NCMAE for detection of HuNoV in sewage.

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Detection of Noroviruses in Ready-To-Eat Foods by Using Carbohydrate-Coated Magnetic Beads

Cited by 48 publications

References 24 publications

Analytical Methods for Food and Environmental Viruses

Analytical Methods for Food and Environmental Viruses

Scientific Opinion on an update on the present knowledge on the occurrence and control of foodborne viruses

Application of a Receptor-Binding Capture Quantitative Reverse Transcription-PCR Assay To Concentrate Human Norovirus from Sewage and To Study the Distribution and Stability of the Virus

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