Detection of Norovirus Antigens from Recombinant Virus-Like Particles and Stool Samples by a Commercial Norovirus Enzyme-Linked Immunosorbent Assay Kit

Okitsu‐Negishi, Shoko; Okame, Michio; Shimizu, Yuko; Phan, Tung Gia; Tomaru, Takeshi; Kamijo, Shigenori; Sato, Takashi; Yagyu, Fumihiro; Müller, Wernér E.G.; Ushijima, Hiroshi

doi:10.1128/jcm.01373-06

Cited by 30 publications

(13 citation statements)

References 19 publications

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“…The previous studies showed that the sensitivities of ELISA and IC ranged from 10 6 to 10 7 copies/g of fecal sample (4,14,25,29), while the sensitivities of the BLEIA ranged from 10 5 to 10 6 copies/g of fecal sample. Second, the BLEIA is more broadly reactive against various genotypes.…”

Section: Discussionmentioning

confidence: 99%

Bioluminescent Enzyme Immunoassay for the Detection of Norovirus Capsid Antigen

Sakamaki

Ohiro

Ito

et al. 2012

Clin Vaccine Immunol

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An ultrasensitive and fully automated bioluminescent enzyme immunoassay (BLEIA) was developed for the detection of norovirus (NV) capsid antigen. In the evaluation tests with recombinant virus-like particles, the BLEIA demonstrated broad reactivity against several NV genotypes (genotypes 1, 3, 4, 7, 8, and 12 in genogroup I [GI] and genotypes 1, 2, 3, 4, 5, 6, 12, and 13 in GII), a wide dose-response range from 0.25 pg/ml to 10,000 pg/ml, and good reproducibility with low coefficients of variation (CVs) (within-run CVs of <2.8%, between-day CVs of <3.7%). In the evaluation tests with NV-positive fecal samples, a good correlation (y ‫؍‬ 0.66x ؊ 3.21, r ‫؍‬ 0.84) between the BLEIA and real-time quantitative reverse transcription-PCR was obtained. Furthermore, in the dilution test with NV specimens, the analytical sensitivity of NV was estimated to be 10 5 to 10 6 copies/g of fecal sample, indicating that the analytical sensitivity of the BLEIA is comparable to that of commercially available molecular methods. All assay steps are fully automated, the turnaround time is 46 min, and the throughput of the assay is 120 tests/h. These results indicate that the BLEIA is potentially useful for the rapid diagnosis of NV in epidemic and sporadic gastroenteritis.

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Section: Discussionmentioning

confidence: 99%

Bioluminescent Enzyme Immunoassay for the Detection of Norovirus Capsid Antigen

Sakamaki

Ohiro

Ito

et al. 2012

Clin Vaccine Immunol

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“…Previous publications have suggested a limited sensitivity of the NoV Ridascreen EIA kit, except for the GII.4 type (Okitsu-Negishi et al 2006, Kirby et al 2010). However, all samples tested by EIA were also tested by RT-PCR, which is a more sensitive method for detecting any NoV genotype.…”

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confidence: 99%

Group A rotavirus and norovirus display sharply distinct seasonal profiles in Belém, northern Brazil

Siqueira

Linhares

Gonçalves

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…Therefore, highly sensitive assays for detecting NoV are required, and automated and rapid diagnostic systems are particularly ideal for the testing of large numbers of specimens from patients and food handlers. Many methods such as the reverse transcription polymerase chain reaction (RT‐PCR), reverse transcription loop‐mediated isothermal amplification (RT‐LAMP), real‐time RT‐PCR, enzyme‐linked immunosorbent assays (ELISA), and immunochromatoassays have been developed and used for this purpose [Kojima et al, ; Kageyama et al, ; Fukuda et al, ; Okitsu‐Negishi et al, ; Mutoh et al, ]. However, these methods are labor‐intensive.…”

Section: Introductionmentioning

confidence: 99%

Clinical evaluation of a bioluminescent enzyme immunoassay for detecting norovirus in fecal specimens from patients with acute gastroenteritis

Shigemoto¹,

Tanizawa²,

Matsuo³

et al. 2013

Journal of Medical Virology

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Noroviruses (NoVs), which belong to the family Caliciviridae, are major causative agents of acute gastroenteritis worldwide. Thus, rapid and highly sensitive assays for detecting NoVs are required. Recently, a bioluminescent enzyme immunoassay (BLEIA) for detecting NoVs in fecal specimens was developed. This new assay was evaluated using fecal specimens obtained from acute gastroenteritis patients. Of the 107 specimens that were found to be NoV-positive by RT-PCR or RT-LAMP, 104 specimens produced positive results in the BLEIA (sensitivity: 96.3%). On the other hand, no false-positive results were observed during the testing of 176 NoV-negative specimens containing group A or C rotaviruses, astroviruses, sapoviruses, adenovirus type 41, bocaviruses, or parechoviruses. Furthermore, the BLEIA was able to detect many NoV genotypes in the tested specimens, including three genotypes from genogroup I (genotypes 1, 4, and 8) and ten genotypes belonging to genogroup II (genotypes 1, 2, 3, 4, 5, 6, 12, 13, 16, and 19). By quantifying the number of NoV genome copies in the clinical specimens tested with the BLEIA, its detection limit was estimated to be 10(6) genome copies per gram of stool and below. Furthermore, as the BLEIA can be performed with an automated device and does not involve complicated procedures it can be used to rapidly test many samples. Therefore, the BLEIA is a rapid and highly sensitive method and could be used as a diagnostic tool at hospitals and clinical laboratories that deal with large numbers of clinical specimens from acute gastroenteritis patients or food handlers. J. Med. Virol. 86:1219-1225, 2014. © 2013 Wiley Periodicals, Inc.

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Detection of Norovirus Antigens from Recombinant Virus-Like Particles and Stool Samples by a Commercial Norovirus Enzyme-Linked Immunosorbent Assay Kit

Cited by 30 publications

References 19 publications

Bioluminescent Enzyme Immunoassay for the Detection of Norovirus Capsid Antigen

Bioluminescent Enzyme Immunoassay for the Detection of Norovirus Capsid Antigen

Group A rotavirus and norovirus display sharply distinct seasonal profiles in Belém, northern Brazil

Clinical evaluation of a bioluminescent enzyme immunoassay for detecting norovirus in fecal specimens from patients with acute gastroenteritis

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