Detection of Nitric Oxide and Determination of Nitrite Concentrations in Arabidopsis thaliana and Azospirilum brasilense

Foresi, Noelia; Correa‐Aragunde, Natalia; Amenta, Melina; Arruebarrena, Andrés; Creus, Cecilia M.; Lamattina, Lorenzo

doi:10.21769/bioprotoc.1765

Cited by 8 publications

(7 citation statements)

References 5 publications

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“…In order to assess the NO releasing potential in Arabidopsis leaves we used Griess reagent for indirect determination of NO through its oxidized nitrite form [ 48 ]. All Arabidopsis leaves treated with the same amounts of synthesized Schiff bases bearing a furoxan moiety were incubated at room temperature for 5-10 min.…”

Section: Resultsmentioning

confidence: 99%

“…Spectrophotometric analysis were performed on the Elisa plate according the reported bioprotocol [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

“…The Arabidopsis leaves were sprayed with the inductor suspensions (at a rate of 1 mL/plant) andcollected after 24 hours. The leaves were washed with distilled water for histochemical analysis of ROS and NO by fluorescence microscopy, or worked-up according to the reported protocol [ 48 ], in order to determine NO releasing potential of new synthesized Schiff bases bearing a furoxan moiety with Griess reagent. As positive control in histochemical analysis by fluorescent microscopy we used plant treated with chitosan solution, 10 μg/mL, and 50 μg/mL respectively, in 0.5% acetic acid solution, buffered to pH 5.6 with NaOH 1 M.…”

Section: Methodsmentioning

confidence: 99%

“…A nitrite standard curve was used to calculate the nitrite concentration in the samples and expressed as μM of nitrite anion. The detection of nitrite concentration in Aradidopsis thaliana leaves was performed according to the reported bioprotocol [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

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Schiff bases containing a furoxan moiety as potential nitric oxide donors in plant tissues

Georgescu¹,

Oancea²,

Georgescu³

et al. 2018

PLoS ONE

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Stable Schiff bases containing a furoxan moiety are synthesized as single regioisomers by the reaction of 3-methyl-2-oxy-furazan-4-carbaldehydewith various amino compounds at room temperature. The structures of synthesized compounds were fully characterized by multinuclear NMR spectroscopy and X-ray crystallography. The effect of synthesized Schiff bases containing a furoxan moiety on biological generation of reactive oxygen species and nitric oxide in plant tissues was investigated for the first time by fluorescence microscopy and the released NO identified as nitrite with Griess reagent. There is a good correlation between the biological generation of NO determined by fluorescence microscopy and with Griess reagent. Some of the synthesized compounds exhibited both nitric oxide and reactive oxygen species generation abilities and represent potential NO donors in plant tissues.

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Section: Resultsmentioning

confidence: 99%

“…Spectrophotometric analysis were performed on the Elisa plate according the reported bioprotocol [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Schiff bases containing a furoxan moiety as potential nitric oxide donors in plant tissues

Georgescu¹,

Oancea²,

Georgescu³

et al. 2018

PLoS ONE

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show abstract

“…The absorbance was measured using a SpectraMax M3 plate reader (wavelength 548 nm). Sodium nitrite (Sigma, UK) at different concentrations was used to create a standard curve (68).…”

Section: Methodsmentioning

confidence: 99%

Activity of Chitosan and Its Derivatives against Leishmania major and Leishmania mexicana In Vitro

Riezk

Raynes

Yardley

et al. 2020

Antimicrob Agents Chemother

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There is an urgent need for safe, efficacious, affordable, and field-adapted drugs for the treatment of cutaneous leishmaniasis, which newly affects around 1.5 million people worldwide annually. Chitosan, a biodegradable cationic polysaccharide, has previously been reported to have antimicrobial, antileishmanial, and immunostimulatory activities. We investigated the in vitro activity of chitosan and several of its derivatives and showed that the pH of the culture medium plays a critical role in antileishmanial activity of chitosan against both extracellular promastigotes and intracellular amastigotes of Leishmania major and Leishmania mexicana. Chitosan and its derivatives were approximately 7 to 20 times more active at pH 6.5 than at pH 7.5, with high-molecular-weight chitosan being the most potent. High-molecular-weight chitosan stimulated the production of nitric oxide and reactive oxygen species by uninfected and Leishmania-infected macrophages in a time-and dose-dependent manner at pH 6.5. Despite the in vitro activation of bone marrow macrophages by chitosan to produce nitric oxide and reactive oxygen species, we showed that the antileishmanial activity of chitosan was not mediated by these metabolites. Finally, we showed that rhodamine-labeled chitosan is taken up by pinocytosis and accumulates in the parasitophorous vacuole of Leishmaniainfected macrophages.

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Nitric oxide–secreting probiotics as sustainable bio-cleaners for reverse osmosis membrane systems

Maitreya

Pal

Qureshi

et al. 2021

Environ Sci Pollut Res

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Membrane biofouling in water purification plants is a serious issue of worldwide concern. Various chemical, physical, and biochemical processes are practised for membrane clean-up. A high-dosage treatment adversely affects the life expectancy of the membrane, and minimum dosage seems unable to deteriorate the biofilms on the membrane. It is reported that quorum quenchers like nitric oxide (NO) disrupt biofilm signals through metabolic rewiring, and also NO is known to be secreted by probiotics (good bacteria). In the present review, it is hypothesized that if probiotic biofilms secreting NO are used, other microbes that aggregate on the filtration membrane could be mitigated. The concept of probiotic administration on filtration membrane seeks to be encouraged because probiotic bacteria will not be hazardous, even if released during filtration. The fundamental motive to present probiotics as a resource for sequestering NO may serve as multifunctional bioweapons for membrane remediation, which will virtually guarantee their long-term sustainability and green approach.

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Detection of Nitric Oxide and Determination of Nitrite Concentrations in Arabidopsis thaliana and Azospirilum brasilense

Cited by 8 publications

References 5 publications

Schiff bases containing a furoxan moiety as potential nitric oxide donors in plant tissues

Schiff bases containing a furoxan moiety as potential nitric oxide donors in plant tissues

Activity of Chitosan and Its Derivatives against Leishmania major and Leishmania mexicana In Vitro

Nitric oxide–secreting probiotics as sustainable bio-cleaners for reverse osmosis membrane systems

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