Detection of new PTEN/MMAC1 mutations in head and neck squamous cell carcinomas with loss of chromosome 10

Poetsch, Micaela; Lorenz, Gerd; Kleist, Britta

doi:10.1016/s0165-4608(01)00509-x

Cited by 66 publications

(57 citation statements)

References 17 publications

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“…In fact, point mutations found in endometrial cancer and Cowden disease lie within this exon, and a mutational hotspot has been described for HNSCC. 30 Although it is possible that mutations on other exons would be missed in our study, our results are in agreement with those reported by Henderson et al 31 who found no point mutations in PTEN gene in HNSCC. On the other hand, the finding of only one case showing PTEN homozygous deletion is in line with the results reported by Chen et al 32 who found no single homozygous deletion in 28 fresh oral squamous cell carcinomas.…”

Section: Discussionsupporting

confidence: 93%

Retracted: Frequent genetic and biochemical alterations of the PI 3‐K/AKT/PTEN pathway in head and neck squamous cell carcinoma

Pedrero

Carracedo

Pinto

et al. 2004

Intl Journal of Cancer

236

163

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Section: Discussionsupporting

confidence: 93%

Retracted: Frequent genetic and biochemical alterations of the PI 3‐K/AKT/PTEN pathway in head and neck squamous cell carcinoma

Pedrero

Carracedo

Pinto

et al. 2004

Intl Journal of Cancer

236

163

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“…Previous reports have not identified mutations in the PTEN gene in this type of cancer 8,19 . However, Poetsch et al 27 demonstrated PTEN mutations in 23% of head and neck SCC tumor samples, and Kurose et al 28 found intragenic PTEN mutations in 15% (3/20) of cervical tumors. PTEN mutations were frequently found in cancers arising from the endometrium 29,30 , brain 31 and prostate 32 .…”

Section: Discussionmentioning

confidence: 99%

PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31

Loures¹,

Cândido²,

Vidigal³

et al. 2014

Rev. Bras. Ginecol. Obstet.

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PURPOSE: To investigate protein expression and mutations in phosphatase and tensin homolog (PTEN) in patients with stage IB cervical squamous cell carcinoma (CSCC) and the association with clinical-pathologic features, tumor p53 expression, cell proliferation and angiogenesis. METHODS: Women with stage IB CSCC (n=20 -Study Group) and uterine myoma (n=20 -Control Group), aged 49.1±1.7 years (mean±standard deviation, range 27-78 years), were prospectively evaluated. Patients with cervical cancer were submitted to Piver-Rutledge class III radical hysterectomy and pelvic lymphadenectomy and patients in the Control Group underwent vaginal hysterectomy. Tissue samples from the procedures were stained with hematoxylin and eosin for histological evaluation. Protein expression was detected by immunohistochemistry. Staining for PTEN, p53, Ki-67 and CD31 was evaluated. The intensity of PTEN immunostaining was estimated by computer-assisted image analysis, based on previously reported protocols. Data were analyzed using the Student's t-test to evaluate significant differences between the groups. Level of significance was set at p<0.05. RESULTS: The PTEN expression intensity was lower in the CSCC group than in the Control (benign cervix) samples (150.5±5.2 versus 204.2±2.6; p<0.001). Our study did not identify any mutations after sequencing all nine PTEN exons. PTEN expression was not associated with tumor expression of p53 (p=0.9), CD31 (p=0.8) or Ki-67 (p=0.3) or clinical-pathologic features in patients with invasive carcinoma of the cervix. CONCLUSIONS: Our findings demonstrate that the PTEN protein expression is significantly diminished in CSCC.

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“…Our results are in agreement with reports showing that in HNSCC, somatic mutations in PTEN gene are infrequent (Henderson et al 1998;Gasparotto et al 1999;Hu et al 1999;Chen et al 2000;Mavros et al 2002). Other studies, however, imply that mutation of PTEN might play a role in the genesis of some HNSCC, ie., carcinomas of the oropharynx, larynx, and hypophaynx (Okami et al 1998;Shao et al 1998;Poetsch et al 2002). Analysis of PTEN expression at protein level is probably more relevant for the elucidation of its role in head and neck tumors.…”

Section: Somatic Mutation In Pten and Lkb1 Genes Is A Rare Event In Lmentioning

confidence: 99%

Human papillomavirus type 16 in head and neck carcinogenesis

2005

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Detection of new PTEN/MMAC1 mutations in head and neck squamous cell carcinomas with loss of chromosome 10

Cited by 66 publications

References 17 publications

Retracted: Frequent genetic and biochemical alterations of the PI 3‐K/AKT/PTEN pathway in head and neck squamous cell carcinoma

Retracted: Frequent genetic and biochemical alterations of the PI 3‐K/AKT/PTEN pathway in head and neck squamous cell carcinoma

PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31

Human papillomavirus type 16 in head and neck carcinogenesis

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