Detection of Neuroblastoma Cells During Clinical Follow Up: Advanced Flow Cytometry and RT-PCR for Tyrosine Hydroxylase Using Both Conventional and Real-Time PCR

Esser, Ruth; Glienke, Wolfgang; Bochennek, Konrad; Erben, Stephanie; Quaiser, Andrea; Pieper, Christian; Eggert, Angelika; Schramm, Alexander; Astrahantseff, Kathy; Hansmann, Martin‐Leo; Schwabe, Dirk; Klingebiel, Thomas; Koehl, Ulrike

doi:10.1055/s-0031-1287842

Cited by 6 publications

(2 citation statements)

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“…5,6 However, black patients seem to have a higher proportion of high-risk disease associated with sperm-associated antigen 16 single nucleotide polymorphism, which carries a poorer prognosis. 7-9 …”

Section: Introductionmentioning

confidence: 99%

Neuroblastoma in Africa: A Survey by the Franco-African Pediatric Oncology Group

Traoré

Eshun

Togo

et al. 2016

JGO

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PurposeNeuroblastoma is a sympathoadrenal lineage neural crest–derived tumor. It is the third most common childhood malignancy in the Western world. Studies from the United States show that black patients with neuroblastoma have a greater proportion of high-risk neuroblastoma with poorer prognosis compared with white patients. In Africa, there are few published data on the epidemiology and management of neuroblastoma. The primary aim of this study was to assess the diagnostic and therapeutic resources available for the management of neuroblastoma within the Franco-African Pediatric Oncology Group (GFAOP).MethodsA survey was conducted in the pediatric oncology centers of the GFAOP. Participating GFAOP centers were Abidjan, Algiers, Bamako, Dakar, Lubumbashi, Lomé, Ouagadougou, Rabat, Tananarive Antananarivo, and Tunis. Questionnaires were sent out by e-mail to the principal investigators at each participating GFAOP center in December 2013.ResultsTen (62%) of 16 GFAOP centers responded to the questionnaire. Neuroblastoma represented only 3% to 5% of childhood cancers in the sub-Saharan African centers, with the exception of Antananarivo, where it represented 7.5%. In contrast, in the northern African centers of Tunis, Rabat, and Algiers, neuroblastoma accounted for 30%,10%, and 7% of childhood cancer, respectively. At initial diagnosis, 50% to 80% of patients had metastatic neuroblastoma in eight of 10 centers.ConclusionBased on this survey, neuroblastoma seems to be less common in sub-Saharan Africa. The proportion of patients with metastatic neuroblastoma seems to be higher than reported in Western countries.

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Section: Introductionmentioning

confidence: 99%

Neuroblastoma in Africa: A Survey by the Franco-African Pediatric Oncology Group

Traoré

Eshun

Togo

et al. 2016

JGO

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“…Thus ESFTs are diagnosed by needle biopsy (and expression using immunohistochemistry), and not resected until after administration of neoadjuvant chemotherapy, such that adequate numbers of fresh cells could not be obtained in primary patient samples [4]. The RT-qPCR or RT-cPCR detection of tyrosine hydroxylase (TH) is used to detect Ewing's sarcoma, nephroblastoma and rhabdomyosarcoma [5]. Pediatric undifferentiated soft tissue sarcomas (USTSs) are composed predominantly of primitive round cell sarcomas, the histogenesis of which is uncertain and their diagnosis and therapy remain a challenge.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Strategies and Treatment for Ewing’s Sarcoma

Todorova¹,

Atanasov²

2012

IJCM

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Ewing's sarcoma is an enigmatic malignancy of progenitor cell origin, driven by transcription factor oncogenic fusions. About 85% of ESFT cases harbor the t(11;22) translocation and express the fusion protein EWS-FLI. Both bone marrow-derived human Mesenchymal stem cells and Neural crest stem cells are permissive for EWS-FLI1 expression that initiates transition to ESFT-like cellular phenotype. Diagnosis of Ewing's tumor is based on pathologic and molecular findings. The hypoxia enhances the malignancy of ESFT invasive capacity. An ALDH high subpopulation of Ewing's sarcoma cells, capable of self-renewal, tumor initiation and resistant to chemotherapy in vitro, are not resistant to YK-4-279. Intensive high-dose chemotherapy followed by stem-cell reconstitution was used for ESFT patients in second remission. Plerixafor in combination with G-CSF is an effective enhance stem cell mobilization regimen for stem cell collection with lowest success rate in patients with neuroblastoma. The ESFT-derived antigens EZH2(666) and CHM1(319) are suitable targets for protective allo-restricted human CD8(+) T-cell responses against non-immunogenic ESFT. Primitive neuroectodermal features and MSC origin are both compatible with G(D2) aberrant expression and explore G(D2) immune targeting in ESFT.

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Utility of Flow Cytometry Analysis in the Detection of Nonhematologic Neoplasms

Nabeel

Alobeid

2023

Clinics in Laboratory Medicine

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Detection of Neuroblastoma Cells During Clinical Follow Up: Advanced Flow Cytometry and RT-PCR for Tyrosine Hydroxylase Using Both Conventional and Real-Time PCR

Abstract: TH RT-qPCR alone is limited for detection of NB cells because of "false positives" in samples from patients with other diseases. Advanced FC may serve as a complementary method to detect residual NB, but needs further confirmation in larger patient cohorts.

Cited by 6 publications

References 10 publications

Neuroblastoma in Africa: A Survey by the Franco-African Pediatric Oncology Group

Neuroblastoma in Africa: A Survey by the Franco-African Pediatric Oncology Group

Diagnostic Strategies and Treatment for Ewing’s Sarcoma

Utility of Flow Cytometry Analysis in the Detection of Nonhematologic Neoplasms

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