Detection of naturally aerosolized Actinobacillus pleuropneumoniae on pig farms by cyclonic air sampling and qPCR

Watt, Anneliese; Browning, Glenn F.; Markham, Philip F.; Marenda, Marc S.

doi:10.1016/j.vetmic.2020.108856

Cited by 7 publications

(8 citation statements)

References 16 publications

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“… The following bioaerosol samplers were used in the literature: filter samplers (Masclaux et al, 2009;Choi, 2004, 2015;Kumari et al, 2016), Coriolis®µ wet cyclones (Bonifait et al, 2014;Kraemer et al, 2019;Watt et al, 2020), impingers , and electrostatic dustfall collectors (Luiken, 2021). While solid samples were acquired from filter samplers and electrostatic dust fall collectors, liquid samples were derived from wet cyclones and impingers.…”

Section: Total Bacterial and Fungal Countsmentioning

confidence: 99%

“… Multiple DNA extraction kits were used, including QIAamp DNA Minikit (Létourneau et al, 2009;Bonifait et al, 2014), Fast Spin Kit (Masclaux et al, 2013), Power Soil Kit Choi, 2014, 2015;Kumari et al, 2016), Qiagen DNA Minikit (Kraemer et al, 2019), Ultra Clean GelSpin DNA Extraction Kit (Watt et al, 2020), and Nucleospin 8 Plant II Kit (Luiken, 2021).…”

Section: Total Bacterial and Fungal Countsmentioning

confidence: 99%

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Particulate Matter in Swine Barns: A Comprehensive Review

Yang¹,

Haleem²,

Osabutey³

et al. 2022

Preprint

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Particulate matter (PM) represents an air quality management challenge for confined swine production systems. Because of the limited space and ventilation rate, PM can reach relatively high concentrations in swine barns. PM in swine barns possesses different physical, chemical, and biological characteristics than that in the atmosphere and other indoor environments. As a result, it exerts different environmental and health effects and creates some unique challenges regarding PM measurement and mitigation. Numerous research efforts have been made, generating massive data and information. However, relevant review reports are sporadic. This study aims to provide an updated comprehensive review of swine barn PM, focusing on publications since 1990. It covers various topics, including PM characteristics, sources, measurement methods, and in-barn mitigation technologies. Since PM in swine barns is of primarily biological origins, bioaerosols are reviewed in great detail. Relevant topics include bacterial/fungal counts, viruses, microbial community composition, antibiotic-resistant bacteria, antibiotic resistance genes, endotoxins, and (1→3)-β-D-glucans. For each topic, existing knowledge is summarized and discussed and knowledge gaps are identified. Overall, PM in swine barns is complicated in chemical and biological composition and highly variable in mass concentrations, size, and microbial abundance. Feed, feces, and skins constitute the major PM sources. Regarding in-barn PM mitigation, four technologies (oil/water sprinkling, ionization, alternation of feed and feeders, and recirculating air filtration) are dominant. However, none of them have been widely used in commercial barns. A collective discussion of major knowledge gaps and future research needs is offered at the end of the report.

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Section: Total Bacterial and Fungal Countsmentioning

confidence: 99%

Section: Total Bacterial and Fungal Countsmentioning

confidence: 99%

Particulate Matter in Swine Barns: A Comprehensive Review

Yang¹,

Haleem²,

Osabutey³

et al. 2022

Preprint

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“…In the past two decades, polymerase chain reaction (PCR)-based molecular detection technology has been widely used in the molecular diagnosis of A. pleuropneumoniae and S. suis. Conventional PCR, PCR-RFLP, PCR-SCAR, double PCR, realtime uorescence quantitative PCR and other technologies are used to amplify and sequence the conserved genes of these two pathogens or the capsular polysaccharide genes speci c to different serotypes to realize the rapid, synchronous or quantitative detection and identi cation of these two pathogens at the gene level (Sassu et al 2018;Watt et al 2020;Okura et al 2014;Nga et al 2011;Tobias et al 2012;Turni et al 2014). PCR-based molecular technology provides an established and reliable method for the isolation and identi cation of A. pleuropneumoniae and S. suis but also has characteristics including extensive requirements for instruments and equipment, experimental conditions and the professional background of laboratory personnel.…”

Section: Introductionmentioning

confidence: 99%

Rapid visual detection of Streptococcus suis and Actinobacillus pleuropneumoniae using duplex recombinase polymerase amplification combined with lateral flow dipsticks

Zhang

Xie

Liu

et al. 2022

Preprint

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We established a rapid detection method based on recombinant enzyme polymerase amplification (RPA) for the simultaneous detection and identification of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) and Streptococcus suis (S. suis). A pair of primers and corresponding probes were designed for the glutamate dehydrogenase (gdh) gene of S. suis and the ApxIV gene of A. pleuropneumoniae to establish a dual RPA-LFD detection method for the rapid identification of S. suis and A. pleuropneumoniae. The specificity test showed that the amplification results for Pasteurella, E. coli, Salmonella, S. aureus, H. parasuis and A. hydrophila were all negative, indicating the good specificity of the method. The sensitivity test showed that the lowest nucleic acid concentration that was detectable with this method was 10− 5 ng/µL, which was significantly higher than that of PCR and RPA-Basic. S. suis and A. pleuropneumoniae reference strains, clinical isolates and 45 clinical lung tissue samples were detected with this method. The results showed that this method detected all reference strains and clinical isolates, which was consistent with the PCR detection results. Among the 45 clinical samples, 19 cases of S. suis and 1 case of A. pleuropneumoniae were detected, and there were no mixed infections. The detection rate was higher than that of bacterial isolation and the conventional PCR method, indicating that this method is very practical and suitable for the rapid clinical detection of S. suis and A. pleuropneumoniae. Compared with the traditional method, the dual RPA-LFD method has several advantages including a high specificity, high sensitivity, fast speed (detection time of 20 min) and minimal requirement for instruments and equipment; in addition, the method can realize the synchronous and rapid detection of S. suis and A. pleuropneumoniae and is helpful for the preliminary screening of clinical diseases.

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“…Actinobacillus pleuropneumoniae is the primary aetiological agent of contagious porcine pleuropneumonia (Dayao et al ., 2016; Watt et al ., 2020) associated with serious economic impact on pig husbandry worldwide (Gottschalk and Broes, 2019). Pigs of 12–16 weeks of age are found to be most susceptible to the disease (Makrai et al ., 2019), where infection may be contracted by direct contact with diseased animals including asymptomatic carriers or by aerosol route within 1–2 m (Watt et al ., 2020). Haemorrhagic necrotizing pneumonia and fibrinous pleuritis are common characteristic features of the disease (Frey, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…carriers or by aerosol route within 1-2 m (Watt et al, 2020). Haemorrhagic necrotizing pneumonia and fibrinous pleuritis are common characteristic features of the disease (Frey, 2019).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Actinobacillus pleuropneumoniae targeting the apxIVA gene for diagnosis of contagious porcine pleuropneumonia in pigs by polymerase spiral reaction

Sarkar

Roychoudhury

Kumar

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: Actinobacillus pleuropneumoniae is the primary aetiological agent of contagious porcine pleuropneumonia associated with serious economic impact on pig husbandry worldwide. Diagnosis of the disease by existing techniques including isolation and identification of bacteria followed by serotyping, serological techniques, conventional PCR, real-time PCR and LAMP assays are cumbersome, time-consuming, costly and not suitable for rapid field application. A novel isothermal polymerase spiral reaction (PSR) is developed for the specific detection of A. pleuropneumoniae in porcine nasal swabs. The technique is highly sensitive, specific, cost-effective and applicable to the field condition for rapid diagnosis of the disease. To the best of our knowledge, this is the first-ever report on the development of PSR for specific detection of A. pleuropneumoniae.

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Detection of naturally aerosolized Actinobacillus pleuropneumoniae on pig farms by cyclonic air sampling and qPCR

Cited by 7 publications

References 16 publications

Particulate Matter in Swine Barns: A Comprehensive Review

Particulate Matter in Swine Barns: A Comprehensive Review

Rapid visual detection of Streptococcus suis and Actinobacillus pleuropneumoniae using duplex recombinase polymerase amplification combined with lateral flow dipsticks

Rapid detection of Actinobacillus pleuropneumoniae targeting the apxIVA gene for diagnosis of contagious porcine pleuropneumonia in pigs by polymerase spiral reaction

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