Detection of naphthalene by real-time immuno-PCR using molecular beacon

Ye, Qi-Yan; Zhuang, Huisheng; Zhou, Chun

doi:10.1016/j.mcp.2008.10.004

Cited by 16 publications

(4 citation statements)

References 40 publications

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“…2,3 Therefore, PAHs have been listed as pollutants of the most concern by both the European Community and the United States Environmental Protection Agency. 4,5 A total of 16 PAHs have been listed as containing hydrocarbons that have 2-6 condensed rings. 6,7 Despite the fact that 5-6-ring PAHs are more toxic, the 2-3-ring PAHs such as naphthalene and anthracene are more important and are of more concern environmentally because of their higher emission and better volatility and solubility in water.…”

mentioning

confidence: 99%

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Zainal

Ahmad

Ngee

2019

J. Electrochem. Soc.

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In this study, electrochemically reduced graphene oxide (ERGO) and 4-tertbutylcalix[4]arene (C4) were deposited on the surface of screen-printed carbon electrodes (SPCE) to detect anthracene in aqueous solution. The formation of electrochemically reduced graphene oxide/calix[4]arene (ERGO/C4) on SPCE was characterized using Raman spectroscopy, FTIR, and FESEM. Electrochemical properties of the modified electrode were investigated by means of CV and DPV. The electrochemical response of anthracene was greatly improved on ERGO/C4-SPCE compared to bare SPCE. Under optimized condition, the anthracene peak showed a good linear relationship at a concentration ranging from 2-8 nM and a detection limit of 0.016 nM. The developed sensor was able to conserve its performance in the presence of various organic compound and inorganic metal ions and also yielded a good comparable result to the conventional method which is HPLC in real sample with acceptable recovery of 96-97%; thus proving to be a promising candidate for rapid, inexpensive, and sensitive determination of anthracene in real samples.

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mentioning

confidence: 99%

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Zainal

Ahmad

Ngee

2019

J. Electrochem. Soc.

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“…On one hand, the extreme sensitivity of the PCR amplification step dramatically improves the sensitivity of the methodology. On the other hand, it leads to more stringent requirements for PD-IPCR in each step of the assay to obtain stable and accurate results [39,40]. The study of PD-IPCR in this research remains at experimental level, while considering the large amount of data (sensitivity, specificity, and robustness analysis) collected in this research and other researches related to immuno-PCR [35,36,39,40], there is an enormous potential for PD-IPCR to be served as an ELISA-enhancing detection tool in routine analysis in the future.…”

Section: Vhh Phage Display-medicated Immune-pcrmentioning

confidence: 99%

Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals

Wang

et al. 2016

Talanta

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“…Recently, Ye and coworkers established a rapid and quantitative technique to detect trace PAHs in contaminated soil and water samples by using a rapid real-time immuno-PCR method. Their study revealed that the real-time immuno-PCR method is highly sensitive and precise; therefore, it is a promising tool in the trace analysis of environmental endocrine disruptors [11]. Their group also developed real-time immuno-PCR to detect toxicological and carcinogenic polychlorinated biphenyls in environmental samples [12].…”

Section: Quantitative Immuno-pcrmentioning

confidence: 99%

Novel methodologies in analysis of small molecule biomarkers and living cells

Chen

Zhu

2014

Tumor Biol.

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Enzyme-linked immuno-sorbent assay (ELISA) is widely used for biomarker detection. A good biomarker can distinguish patients from healthy or benign diseases. However, the ELISA method is not suitable for small molecule or trace substance detection. Along with the development of new technologies, an increasing level of biomaterials, especially small molecules, will be identified as novel biomarkers. Quantitative immuno-PCR, chromatography-mass spectrometry, and nucleic acid aptamer are emerging methodologies for detection of small molecule biomarkers, even in living cells. In this review, we focus on these novel technologies and their potential for small molecule biomarkers and living cell analysis.

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Detection of naphthalene by real-time immuno-PCR using molecular beacon

Cited by 16 publications

References 40 publications

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Surface Modification of Screen-Printed Carbon Electrode (SPCE) with Calixarene-Functionalized Electrochemically Reduced Graphene Oxide (ERGO/C4) in the Electrochemical Detection of Anthracene

Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals

Novel methodologies in analysis of small molecule biomarkers and living cells

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