Detection of Nano-Oxidation Sites on the Surface of Hemoglobin Crystals Using Tip-Enhanced Raman Scattering

Wood, Bayden Robert; Asghari-Khiavi, Mehdi; Bailo, Elena; McNaughton, Donald; Deckert, Volker

doi:10.1021/nl2044106

Cited by 62 publications

(60 citation statements)

References 36 publications

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“…The Raman spectra of Hb, Mb, cyt c and HRP (EC 1.11.1.7) are well known therefore, here we focus on the comparison between the most prominent bands of the Resonance Raman spectra of standard compounds measured with the use of 488 nm and 532 nm excitation wavelengths (Figs. and ).…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopy of proteins: a review

Ryguła

Majzner

Marzec

et al. 2013

J Raman Spectroscopy

867

790

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In this work, 26 proteins of different structure, function and properties are investigated by Raman spectroscopy with 488, 532 and 1064 nm laser lines. The excitation lines were chosen in NIR and Vis range as the most common and to show the difference due to normal and resonance effect, sometimes accompanied by the fluorescence. The selected proteins were divided, according to the Structural Classification of Proteins, into four classes according to their secondary structure, i.e. α‐helical (α), β‐sheet (β), mixed structures (α/β, α + β, s) and others. For all compounds, FT‐Raman and two Vis spectra are presented along with the detailed band assignment. To the best of our knowledge, this is the first review showing the potential of Raman spectroscopy for the measurement and analysis of such a large collection of individual proteins. This work can serve as a comprehensive vibrational spectra library, based on our and previous Raman measurements. Copyright © 2013 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Raman spectroscopy of proteins: a review

Ryguła

Majzner

Marzec

et al. 2013

J Raman Spectroscopy

867

790

View full text Add to dashboard Cite

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“…Other bacteria-derived samples, such as isolated membrane patches from Halobacterium salinarum have also been measured with TERS, with different patches able to be distinguished [373]. Wood et al demonstrated measurement of hemozoin crystals in sectioned erythrocytes which had been infected with malaria parasites [374], and were also able to identify nano-oxidation sites with TERS [375]. [353]).…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

254

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…To date, TERS has shown wide application in fields, such as surface science, 1-4 nanomaterials, 5-8 biological systems, [9][10][11][12][13] catalysis, 4, 14-16 and molecular electronics. To date, TERS has shown wide application in fields, such as surface science, 1-4 nanomaterials, 5-8 biological systems, [9][10][11][12][13] catalysis, 4, 14-16 and molecular electronics.…”

Section: Introductionmentioning

confidence: 99%

Rational fabrication of a gold-coated AFM TERS tip by pulsed electrodeposition

et al. 2015

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Reproducible fabrication of sharp gold- or silver-coated tips has become the bottleneck issue in tip-enhanced Raman spectroscopy, especially for atomic force microscopy (AFM)-based TERS. Herein, we developed a novel method based on pulsed electrodeposition to coat a thin gold layer over atomic force microscopy (AFM) tips to produce plasmonic TERS tips with high reproducibility. We systematically investigated the influence of the deposition potential and step time on the surface roughness and sharpness. This method allows the rational control of the radii of gold-coated TERS tips from a few to hundreds of nanometers, which allows us to systematically study the dependence of the TERS enhancement on the radius of the gold-coated AFM tip. The maximum TERS enhancement was achieved for the tip radius in the range of 60-75 nm in the gap mode. The coated gold layer has a strong adhesion with the silicon tip surface, which is highly stable in water, showing the great potential for application in the aqueous environment.

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Detection of Nano-Oxidation Sites on the Surface of Hemoglobin Crystals Using Tip-Enhanced Raman Scattering

Cited by 62 publications

References 36 publications

Raman spectroscopy of proteins: a review

Raman spectroscopy of proteins: a review

Raman spectroscopy: techniques and applications in the life sciences

Rational fabrication of a gold-coated AFM TERS tip by pulsed electrodeposition

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