Detection of mycotoxins trough different analytical methods

Abstract

In sti tu te of Ani mal Sci en ce Poc hiv ka 1, Ko stin brod 2232, Bul ga ria DE TEC TION OF MYCO TO XINS TRO UGH DIF FE RENT ANALYTI CAL MET HODS AB STRACT: Myco to xins are se con dary me ta bo li tes pro du ced by fun gi which can af fect a va ri ety of fe ed stuffs. The se com po unds eli cit to xi co lo gi cal ef fects which re pre sent risk for both hu mans and ani mals. The ir to xi city oc curs at very low con cen tra ti ons, the refo re the re is a need for sen si ti ve and re li a ble met hods for the ir de tec tion. This re vi ew aims to eva lu a te clas si cal and emer ging met hods for the analysis of myco to xins con cer ning the ir advan ta ges and di sa dvan ta ges. Cur rently, se ve ral sen si ti ve met hods ba sed on chro ma to graphic or im mu noc he mi cal tec hni que are com mer ci ally ava i la ble. Espe ci ally wi dely are used dif fe rent chro ma to grap hic met hods for qu an ti ta ti ve de ter mi na tion of myco to xins, in clu ding gas-chr o ma to graphy (GC) and high-per for man ce li qu id chr o ma to graphy (HPLC) co u pled with ul tra vi o let, f lu o re scen ce or MS de tec tors. Li qu id chro ma to graphy-tan dem mass spectro me try (LC-MS/MS) is used as a pro mi sing tec hni que for scre e ning, iden ti fi ca tion and qu an ti ta ti ve de ter mi na tion of a lar ge num ber of myco to xins. Im mu no me tric as says, such as enzyme-lin ked im mu no sor bent as says (ELI SA), are fre qu ently used for scre e ning pu r po ses. On the ot her hand, a va ri ety of emer ging met hods ha ve been pro po sed. They are ba sed on no vel tec hno lo gi es, in clu ding im mu noc hro ma to graphy (i.e. la te ral f low de vi ces), f lu o rescen ce po la ri za tion im mu no as says (FPIA), in fra red spec tro scopy (FT-NIR), mo le cu larly im prin ted polymers (MIPs), and op ti cal bi o sen sors. In ad di tion, du ring the last years, the hig hlight was put on na no sca le ma te ri als in clu ded in bi o sen sors, which are so me of the smart de vi ces used for de ter mi na tion of myco to xins.