“…Mycoplasmas were detected in the LSCC-H 32 culture prior to, during and after decontamination by culture in liquid media supplemented with glucose, urea or arginine, culture in a solid medium Ourmanova and Machatkova 1986), the fluorescence method by Chen (1977), modified by Machatkova et al (1986) and scanning electron microscope Tesla BS 300 (Fischer et al 1991). Indirect immunofluorescence method (Rosendal and Black 1972) was used for the determination of mycoplasma species.…”