Detection of Mycoplasma Synoviae and Other Pathogens in Laying Hens with Respiratory Signs in the Rearing and Production Phases

Silva, R. L; Figueira, Arthur de Almeida; Silva, M. M; Dias, Thomas Salles; Machado, Leandro S.; Soares, Nilce Maria; Nascimento, E. R.; Pereira, Vla

doi:10.1590/1806-9061-2020-1318

Cited by 3 publications

(1 citation statement)

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“…Moreover, isolates can be obtained from clinical material only during the acute period of the disease. These microorganisms are often accompanied by a co-infection with other pathogens such as E. coli , P. multocida , Bordetella avium , Mycoplasma gallisepticum , etc., which also complicates the isolation of pure cultures and diagnosis of the diseases [ 15 , 16 ]. An alternative approach to rapid diagnostics of infectious coryza and ornithobacteriosis is the use of PCR techniques.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of PCR Diagnostic Assays for Detection of Avibacterium paragallinarum and Ornithobacterium rhinotracheale

Krylova,

Bogomazova,

Kirsanova

et al. 2023

Veterinary Sciences

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PCR is the most effective method for detecting difficult-to-cultivate pathogens and pathogens that are part of mixed infections in animals, such as Ornithobacterium rhinotracheale, which causes bird ornithobacteriosis, or Avibacterium paragallinarum, which causes infectious coryza. In this work, we developed and validated two efficient and sensitive diagnostic assays for the rapid and accurate detection of A. paragallinarum and O. rhinotracheale DNA in bacterial isolates and clinical samples using real-time PCR with TaqMan-like probes. When designing the PCR assays, we performed in silico analysis, optimized DNA isolation methods and PCR conditions, and assessed the analytical and diagnostic performance of PCR. We designed primers and probes that have no mismatches with published whole-genome sequences of bacteria. The optimization of conditions showed that the PCR assays are sufficiently robust to changes in temperature and oligonucleotide concentration. The validation showed that the developed assays have high analytical and diagnostic sensitivity and specificity. These assays are expected to improve the differential diagnosis of respiratory diseases in chickens and turkeys.

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Section: Introductionmentioning

confidence: 99%