Detection of Mycobacterium avium subspecies paratuberculosis in pasteurized milk by IS900 PCR and culture method

Anzabi, Younes; Hanifian, Shahram

doi:10.5897/ajmr11.1305

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Moreover, polyphenols contained in fruit, and at high concentrations in green tea, can help to increase the levels of antioxidants and attenuate severity of colitis in the analogous way to sulfasalazine, reducing the rate of IBD in Iranians [43, 47]. The result of this study proposes that pasteurized milk would need to be considered as a weak transmission vector confirming the previous report [48] and is in line with other reports. Data regarding the intake of dairy products were not available for the enrolled patients; consumption of processed cheese has been significantly associated with CD and its evaluation in Iranian cohort would be worthwhile [49].…”

Section: Discussionsupporting

confidence: 88%

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

et al. 2017

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BackgroundInflammatory bowel disease (IBD) is described as a relapsing condition with high morbidity and uncertain complex pathogenesis. The association of Mycobacterium avium ssp. paratuberculosis (MAP) with Crohn’s disease (CD) in human has been debated for decades, however there is no confirmed data to verify such relations in Iran. The aim of this study was to investigate risk factors and a possible role of MAP in Iranian patients with CD.MethodsAnti-MAP antibodies were detected in serum of IBD patients and subjects without IBD (nIBD) through ELISA; MAP DNA and viable MAP cells were identified in patients’ biopsies through nested PCR and direct culture methods, respectively. Principal component analysis (PCA) was used to investigate the risk factors in relation to IBD and MAP infection.ResultsPositivity for IS900 PCR was detected in 64% (n = 18) of CD, 33% (n = 10) of ulcerative colitis (UC) and 9.7% (n = 6) of nIBD samples. Live MAP cells were isolated from biopsies of 2 CD patients only. Among 28 patients with CD, 46% (n = 13) and 39% (n = 11) were positive for antibodies against MAP3865c133–141 and MAP3865c125–133 peptides, respectively, whereas much lower seroreactivity was detected in UC subjects accounting for 3% (n = 1) for MAP3865c133–141 and 16.7% (n = 5) for MAP3865c125–133. A high immune reactivity to MAP epitopes among CD patients was positively correlated with consumption of fast food meals and IBD familiarity. For both CD and UC, breastfeeding period and consumption of fruit/vegetables presented negative correlation with the presence of anti-MAP antibodies.ConclusionsThis study provided evidences that high prevalence of MAP DNA and anti-MAP antibodies in CD patients is significantly associated with the development of CD. Despite the role of several factors contributing to IBD, the presence of MAP DNA and anti-MAP antibodies in Iranian CD patients highlights a possible transmission of MAP from animal-derived products to humans.

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Section: Discussionsupporting

confidence: 88%

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

et al. 2017

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“…Bharathy et al have detected MAP DNA in milk samples of goats [10]. This organism is a resistant bacterium to pasteurization temperature for having thick lipid cell wall [66, 67]. Consumption of milk and different dairy products like cheese can increase the risk of transmission to human [10, 21–23, 33, 67].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Mycobacterium avium subspecies paratuberculosis IS 900 DNA in biopsy tissues from patients with Crohn’s disease: histopathological and molecular comparison with Johne’s disease in Fars province of Iran

2019

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BackgroundCrohn’s disease is a chronic enteritis of humans that affects the gastrointestinal tract, especially the terminal ileum, cecum and colon. The etiology of this disease is still unknown but seems to be multifactorial. There are reports about the potential link between Crohn’s disease in humans and the causative agent of Johne’s disease in ruminants. Because of the prevalence of Johne’s disease in the Fars Province of Iran, the aim of this study was to investigate the prevalence of MAP in the biopsy tissues of patients affected by Crohn’s disease in this area.MethodsThe study was performed from April 2015 to June 2017 at Namazi Hospital, Shiraz University of Medical Sciences, and School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Intestinal biopsies of 30 patients (12 male and 18 female; mean age, 34 years; range 4–77 years) with the confirmed diagnosis of Crohn’s disease and 30 patients diagnosed as non-inflammatory bowel disease (19 male and 11 female; mean age, 38 years; range 13–68 years) were studied by molecular, histopathological and histochemical methods. Also, similar numbers of adult goats affected by Johne’s disease were studied, comparatively. DNA extractions of tissue specimens were subjected to PCR to amplify a 413-bp sequence of the IS900 gene.ResultsUsing IS900-PCR, the overall prevalence of MAP in patients affected by Crohn’s disease and non-inflammatory bowel disease were 47 and 13%, respectively. In addition, the prevalence of MAP in goats affected by Johne’s disease was 70%. Using acid-fast histochemical staining, only 7% of Crohn’s disease patients were weakly positive as paucibacillary and 43% of Johne’s disease cases were moderate to strongly positive as multibacillary. Histopathologically, granulomatous enteritis (83 and 90%), lymphoplasmacytic enteritis (17 and 14%), edema and lymphangiectasia (67 and 96%), and vasculitis (20 and 73%) were common findings in Crohn’s and Johne’s diseases, respectively.ConclusionOur findings demonstrate a remarkable association between MAP and CD in this population, and support an etiologic relationship between MAP infection in humans and the development of CD. MAP infection in human tissue may display species-specific pathologic findings, as occurs with other zoonotic pathogens.

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Mycobacterium avium subsp. paratuberculosis – An Overview of the Publications from 2011 to 2016

Dziedzinská

Slaná

2017

Curr Clin Micro Rpt

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Detection of Mycobacterium avium subspecies paratuberculosis in pasteurized milk by IS900 PCR and culture method

Cited by 6 publications

References 28 publications

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

Prevalence of Mycobacterium avium subspecies paratuberculosis IS 900 DNA in biopsy tissues from patients with Crohn’s disease: histopathological and molecular comparison with Johne’s disease in Fars province of Iran

Mycobacterium avium subsp. paratuberculosis – An Overview of the Publications from 2011 to 2016

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