Detection of mycobacterial infection in non-human primates using the Xpert MTB/RIF molecular assay

Silva, Duanne Alves da; Rêgo, Amanda M.; Ferreira, Nicole Victor; Andrade, Manoela A.S. de; Campelo, Aline R.; Caldas, Paulo César S.; Pereira, Márcia A. S.; Redner, Paulo; Pina, Lucindo Cardoso de; Resende, F.C.; Pissinatti, Thalita A.; Lopes, Claudia A.A.; Kugelmeier, Tatiana; Perea, Javier Adolfo Sarria; Souza, Igo Vieira de; Silva, Fábio A. da; Campos, Carla Fonseca Alves; Montes, Fátima Cristina Onofre Fandinho; Antunes, L. Caetano M.

doi:10.1016/j.tube.2017.08.003

Cited by 4 publications

(1 citation statement)

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“…All samples are also sent for direct smear microscopy with Ziehl-Nielsen staining to identify acid-resistant bacilli and bacterial culture in Löwenstein-Jensen medium and incubated for up to 72 days at 37°C in a biological safety cabinet class 3. The Mycobacterium tuberculosis complex species in positive cultures were identi ed using an immunochromatographic assay with the MPT64 marker (Silva et al, 2017).…”

Section: Bacterial Culturementioning

confidence: 99%

Pulmonary granuloma is not always the tuberculosis hallmark: pathological findings of different tuberculosis stages in New and Old World Nonhuman Primates naturally infected with the Mycobacterium tuberculosis Complex

Pereira¹,

Lopes²,

Pissinatti³

et al. 2021

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Herein we present the pathological findings of different tuberculosis stages in Old and New World monkeys kept under human care in Rio de Janeiro, Brazil and naturally infected with Mycobacterium tuberculosis Complex. Fifteen nonhuman primates from five different colonies were incorporated into the study. There are 60% (9/15) Old World Monkeys and 40% (6/15) New World Monkeys. According to the gross and histopathologic findings, the lesions in nonhuman primates of this study are classified into the chronic-active, extrapulmonary, early-activation or latent-reactivation tuberculosis stage. Among the Old World Monkey, 66.7% (6/9) of nonhuman primates, all rhesus monkeys (Macaca mulatta), showed severe granulomatous pneumonia. In all Old World Monkeys cases, typical granulomas were seen in at least one organ regardless of the stage of the disease. In the New World Monkeys, the typical pulmonary granulomas were seen in 16.7% (1/6) of the cases, just in the latent-reactivation stage in Uta Hick’s Bearded Saki (Chiropotes utahickae). In this study, 66.7% (6/9) of Old World Monkeys (OWM) and 83.3% (5/6) of New World Monkeys (NWM) showed pulmonary changes at the histological evaluation. The tuberculosis diagnosis in the nonhuman primates in this study was based on pathological, immunohistochemical, molecular, and bacteriological culture. Although the typical presentation was observed in some cases, the absence of pulmonary granuloma did not exclude the tuberculosis occurrence in nonhuman primates of the Old and New World. Tuberculosis should be included as a cause of interstitial pneumonia with foamy macrophages infiltration in the New World nonhuman primates. Due to the high sensitivity of immunohistochemistry with Anti-Mycobacterium tuberculosis, we suggest the addition of this technique as a diagnostic tool of tuberculosis in the nonhuman primates even when the typical changes are not seen.

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Section: Bacterial Culturementioning

confidence: 99%