Detection of multiple species of human <i>Paragonimus</i> from Mexico using morphological data and molecular barcodes

López-Caballero, Jorge; Oceguera‐Figueroa, Alejandro; León‐Régàgnon, Virginia

doi:10.1111/1755-0998.12093

Cited by 23 publications

(15 citation statements)

References 31 publications

(34 reference statements)

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“…; López‐Caballero et al . ). However, the success rate was only 5% in cestodes (1/20 specimens) and 40% in digeneans (231/572 specimens; see Table S1 (Supporting information) in Moszczynska et al .…”

Section: Introductionmentioning

confidence: 97%

New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)

Steenkiste

Locke

Castelin

et al. 2014

Molecular Ecology Resources

118

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Digeneans and cestodes are species-rich taxa and can seriously impact human health, fisheries, aqua- and agriculture, and wildlife conservation and management. DNA barcoding using the COI Folmer region could be applied for species detection and identification, but both 'universal' and taxon-specific COI primers fail to amplify in many flatworm taxa. We found that high levels of nucleotide variation at priming sites made it unrealistic to design primers targeting all flatworms. We developed new degenerate primers that enabled acquisition of the COI barcode region from 100% of specimens tested (n = 46), representing 23 families of digeneans and 6 orders of cestodes. This high success rate represents an improvement over existing methods. Primers and methods provided here are critical pieces towards redressing the current paucity of COI barcodes for these taxa in public databases.

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Section: Introductionmentioning

confidence: 97%

New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)

Steenkiste

Locke

Castelin

et al. 2014

Molecular Ecology Resources

118

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“…In the Amazon, studies of metacercariae from Mo- reirocarcinus emarginatus confirmed the presence of P. mexicanus. A recent publication studying Paragonimus species from Mexico using morphological and molecular approaches identified three distinct species following comparisons with the DNA sequences from the species found in Ecuador ( López-Caballero et al 2013) . The authors of the publication therefore suggested resurrecting the name P. ecuadoriensis for species in South America.…”

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confidence: 99%

Current status of Paragonimus and paragonimiasis in Ecuador

Calvopiña

Romero

Castañeda

et al. 2014

Mem. Inst. Oswaldo Cruz

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A review of national and international publications on paragonimiasis in Ecuador, epidemiological records from the Ministry of Public Health and unpublished research data was conducted to summarise the current status of the parasite/disease. The purpose of the review is to educate physicians, policy-makers and health providers on the status of the disease and to stimulate scientific investigators to conduct further research. Paragonimiasis was first diagnosed in Ecuador 94 years ago and it is endemic to both tropical and subtropical regions in 19 of 24 provinces in the Pacific Coast and Amazon regions. Paragonimus mexicanus is the only known species in the country, with the mollusc Aroapyrgus colombiensis and the crabs Moreirocarcinus emarginatus, Hypolobocera chilensis and Hypolobocera aequatorialis being the primary and secondary intermediate hosts, respectively. Recent studies found P. mexicanus metacercariae in Trichodactylus faxoni crabs of the northern Amazon. Chronic pulmonary paragonimiasis is commonly misdiagnosed and treated as tuberculosis and although studies have demonstrated the efficacy of praziquantel and triclabendazole for the treatment of human infections, neither drug is available in Ecuador. Official data recorded from 1978-2007 indicate an annual incidence of 85.5 cases throughout the 19 provinces, with an estimated 17.2% of the population at risk of infection. There are no current data on the incidence/prevalence of infection, nor is there a national control programme.

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“…Paragonimus africanus therefore appears to be genetically homogeneous across Nigeria and Cameroon, albeit based on a limited number of samples and only one genetic locus. Paragonimus mexicanus, previously considered the sole etiological agent of paragonimiasis in the Americas, is now believed to include cryptic species ( López-Caballero et al., 2013 ). Further sampling may indicate similar genetic diversity in P. africanus .…”

Section: Discussionmentioning

confidence: 99%

Lung fluke (Paragonimus africanus) infects Nigerian red-capped mangabeys and causes respiratory disease

Friant

Brown

Saari

et al. 2015

International Journal for Parasitology: Parasites and Wildlife

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Eggs of the lung fluke genus Paragonimus were detected in red-capped mangabeys (Cercocebus torquatus) in Nigeria. We assess the role of these primates as potential sylvatic hosts and the clinical effects of the parasite on monkeys. DNA sequenced from eggs in feces were 100% identical in the ITS2 region to Paragonimus africanus sequences from humans in Cameroon. Paragonimus-positive monkeys coughed more than uninfected monkeys. Experimental de-worming led to reduction in parasite intensity and a corresponding reduction of coughing to baseline levels in infected monkeys. This report provides the first evidence of Paragonimus sp. in C. torquatus, of P. africanus in Nigerian wildlife, and the first molecular evidence of the parasite in African wildlife. Coughing, sometimes interpreted as a communication behavior in primates, can actually indicate infection with lung parasites. Observations of coughing in primates may, in turn, provide a useful mechanism for surveillance of Paragonimus spp, which are re-emerging human pathogens, in wildlife reservoirs.

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Detection of multiple species of human Paragonimus from Mexico using morphological data and molecular barcodes

Cited by 23 publications

References 31 publications

New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)

New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes)

Current status of Paragonimus and paragonimiasis in Ecuador

Lung fluke (Paragonimus africanus) infects Nigerian red-capped mangabeys and causes respiratory disease

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