Detection of multidrug resistance and extensively drug resistance among smear-negative extrapulmonary tuberculosis cases in a reference laboratory

Sharma, Srashty; Hanif, Mahmud; Chopra, K.K.; Sharma, Mukesh; Dwivedi, Kaushal Kumar; Sidiq, Zeeshan; Vashistha, Himanshu; Ahmed, Vasim; Dubey, Manoj Kumar

doi:10.4103/bbrj.bbrj_48_18

Cited by 5 publications

(4 citation statements)

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“…In our study, the proportion of MDR-TB among new and previously treated patients was 9.3% [14] in EPTB patients. Previous studies reported from India indicated a higher MDR-TB cases proportion than our findings [25,27,28]. In contrast, two studies from Korea [26,35] one study from Thailand [24], and one study from Ethiopia [23] have reported a lower MDR-TB proportion than our findings.…”

Section: Discussioncontrasting

confidence: 97%

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Molecular characterization and drug resistance patterns of Mycobacterium tuberculosis complex in extrapulmonary tuberculosis patients in Addis Ababa, Ethiopia

et al. 2020

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Background Molecular characterization of Mycobacterium tuberculosis (MTB) is important to understand the pathogenesis, diagnosis, treatment, and prevention of tuberculosis (TB). However, there is limited information on molecular characteristics and drug-resistant patterns of MTB in patients with extra-pulmonary tuberculosis (EPTB) in Ethiopia. Thus, this study aimed to determine the molecular characteristics and drug resistance patterns of MTB in patients with EPTB in Addis Ababa, Ethiopia. Methods This study was conducted on frozen stored isolates of EPTB survey conducted in Addis Ababa, Ethiopia. A drug susceptibility test was performed using BACTEC-MGIT 960. Species and strain identification were performed using the Geno-Type MTBC and spoligotyping technique, respectively. Data were entered into the MIRU-VNTRplus database to assess the spoligotype patterns of MTB. Analysis was performed using SPSS version 23, and participants’ characteristics were presented by numbers and proportions. Results Of 151 MTB isolates, 29 (19.2%) were resistant to at least one drug. The highest proportion of isolates was resistant to Isoniazid (14.6%) and Pyrazinamide (14.6%). Nine percent of isolates had multidrug-resistant TB (MDR-TB), and 21.4% of them had pre-extensively drug-resistant TB (pre-XDR-TB). Among the 151 MTB isolates characterized by spoligotyping, 142 (94.6%) had known patterns, while 9 (6.0%) isolates were not matched with the MIRU-VNTRplus spoligotype database. Of the isolates which had known patterns, 2% was M.bovis while 98% M. tuberculosis. Forty-one different spoligotype patterns were identified. The most frequently identified SpolDB4 (SIT) wereSIT149 (21.2%), SIT53 (14.6%) and SIT26 (9.6%). The predominant genotypes identified were T (53.6%), Central Asia Strain (19.2%) and Haarlem (9.9%). Conclusion The present study showed a high proportion of MDR-TB and pre-XDR-TB among EPTB patients. The strains were mostly grouped into SIT149, SIT53, and SIT26. The T family lineage was the most prevalent genotype. MDR-TB and pre-XDR-TB prevention is required to combat these strains in EPTB. A large scale study is required to describe the molecular characteristics and drug resistance patterns of MTB isolates in EPTB patients.

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Section: Discussioncontrasting

confidence: 97%

“…This proportion is higher than the earlier result reported from Ethiopia [23] and Thailand [24]. However, our finding is similar to the results reported in India [25] and South Korea [26]. Another two studies reported from India indicated higher proportions of INH resistance (>30%) [27,28] than our finding.…”

Section: Discussionsupporting

confidence: 78%

Molecular characterization and drug resistance patterns of Mycobacterium tuberculosis complex in extrapulmonary tuberculosis patients in Addis Ababa, Ethiopia

et al. 2020

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“…The incidence of MDR‐GITB is reported to range from 4% to 13.9% in India 11,27 . The incidence of MDR in EPTB, taken together, varies from 4.3% to 22% in India, 19,28,29 12.5% in Nepal, 30 9.2% in Ethiopia, 18 and 21% in Pakistan 31 …”

Section: Discussionmentioning

confidence: 99%

“…11,27 The incidence of MDR-GITB is reported to range from 4% to 13.9% in India. 11,27 The incidence of MDR in EPTB, taken together, varies from 4.3% to 22% in India, 19,28,29 12.5% in Nepal, 30 9.2% in Ethiopia, 18 and 21% in Pakistan. 31 The results of Rif and Inh susceptibility, as produced by MTBDRplus in the present study, were 100% concordant with both pDST and gene sequencing, thereby producing a sensitivity and specificity of 100% in detecting RifR and InhR.…”

Section: Discussionmentioning

confidence: 99%

MTBDRplus and MTBDRsl for simultaneous diagnosis of gastrointestinal tuberculosis and detection of first‐line and second‐line drug resistance

Sharma

et al. 2023

J of Gastro and Hepatol

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Background and Aim: Emergence of drug resistance, especially to second-line drugs, hampers tuberculosis elimination efforts. The present study aimed to evaluate MTBDRplus and MTBDRsl assays for detecting first-line and second-line drug resistance, respectively, in gastrointestinal tuberculosis (GITB). Methods: Thirty ileocecal biopsy specimens, processed in the Department of Microbiology between 2012 and 2022, that showed growth of Mycobacterium tuberculosis on culture were included in the study. DNA, extracted from culture, was subjected to MTBDRplus and MTBDRsl (Hain Lifescience GmbH, Nehren, Germany), following manufacturer's instructions. Their performance was compared against phenotypic drug susceptibility testing (pDST) and gene sequencing. Results: Out of the 30 specimens, 4 (13.33%) were mono-isoniazid resistant, 4 (13.33%) were multidrug resistant (MDR), 2 (6.67%) were pre-extensively drug resistant (pre-XDR), and 2 (6.67%) were mono-fluoroquinolone resistant. The results were 100% concordant with pDST and gene sequencing. Conclusions: In the wake of growing drug resistance in all forms of extrapulmonary tuberculosis, including GITB, MTBDRplus and MTBDRsl are reliable tools for screening of resistance to both first-line and second-line drugs.

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The pili at genomic level

Farnia,

Ghanavi,

Aghajani

et al. 2024

Pili in Mycobacterium Tuberculosis

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Detection of multidrug resistance and extensively drug resistance among smear-negative extrapulmonary tuberculosis cases in a reference laboratory

Cited by 5 publications

References 0 publications

Molecular characterization and drug resistance patterns of Mycobacterium tuberculosis complex in extrapulmonary tuberculosis patients in Addis Ababa, Ethiopia

Molecular characterization and drug resistance patterns of Mycobacterium tuberculosis complex in extrapulmonary tuberculosis patients in Addis Ababa, Ethiopia

MTBDRplus and MTBDRsl for simultaneous diagnosis of gastrointestinal tuberculosis and detection of first‐line and second‐line drug resistance

The pili at genomic level

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