“…This may be caused by de novo or mosaic de novo mutation less than 20%that cannot be detected by Sanger sequencing. 9 Such false-positive events from the WES in PB1 and PB2 may be caused by misaligning sequencing reads to a reference sequence (RefSeq). Moreover, inaccuracies or biases of the RefSeq, compared to a specific local population, are other sources of false-positive genotype calls in next-generation sequencing (NGS) data.…”