Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy

Yu, Tao; Rong, Zhen; Gu, Zhixia; Wei, Hongjuan; Wang, Yunxiang; Song, Rui; Wang, Shengqi; Wang, Shumei

doi:10.1039/d4ra02049a

RSC Adv.

2024

DOI: 10.1039/d4ra02049a

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Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy

Tao Yu,

Zhen Rong,

Zhixia Gu

et al.

Abstract: A convenient and sensitive single-step RPA-CRISPR/Cas12a assay was developed for point-of-care detection of monkeypox virus in clinical samples.

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2024

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Cited by 2 publications

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The current status and future prospects of CRISPR-based detection of monkeypox virus: A review

Chen,

Zhao,

et al. 2024

Analytica Chimica Acta

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The current status and future prospects of CRISPR-based detection of monkeypox virus: A review

Chen,

Zhao,

et al. 2024

Analytica Chimica Acta

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Graphene-Based Virus Enrichment Protocol Increases the Detection Sensitivity of Human Norovirus in Strawberry and Oyster Samples

Zhou,

Jin,

Yin

et al. 2024

Foods

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Human noroviruses (HuNoVs), the most prevalent viral contaminant in food, account for a substantial proportion of nonbacterial gastroenteritis cases. Extensive work has been focused on the diagnosis of HuNoVs in clinical samples, whereas the availability of sensitive detection methods for their detection in food is lacking. Here, we developed a virus enrichment approach utilizing graphene-based nanocomposites (CTAB-rGO-Fe3O4) that does not rely on large instruments and is suitable for on-site food pretreatment. The recovery efficiency of the developed virus enrichment procedure for serially diluted GII.4 norovirus ranged from 10.06 to 72.67% in strawberries and from 2.66 to 79.65% in oysters. Furthermore, we developed a real-time recombinase polymerase amplification (real-time RPA) assay, which can detect as low as 1.22 genome copies µL−1 of recombinant plasmid standard and has no cross-reactivity with genomes of astrovirus, rotavirus, adenovirus, and MS2 bacteriophage. Notably, the combined virus enrichment and real-time RPA detection assay enhanced the detection limits to 2.84 and 37.5 genome copies g−1 in strawberries and oysters, respectively, compared to those of qPCR. Our strategy, the graphene-based virus enrichment method combined with real-time RPA, presents a promising tool for sensitively detecting HuNoVs in food samples.

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Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy

Abstract: A convenient and sensitive single-step RPA-CRISPR/Cas12a assay was developed for point-of-care detection of monkeypox virus in clinical samples.

Cited by 2 publications

References 39 publications

The current status and future prospects of CRISPR-based detection of monkeypox virus: A review

The current status and future prospects of CRISPR-based detection of monkeypox virus: A review

Graphene-Based Virus Enrichment Protocol Increases the Detection Sensitivity of Human Norovirus in Strawberry and Oyster Samples

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