Detection of Microsporidia and Identification of <i>Enterocytozoon bieneusi</i> in Surface Water by Filtration Followed by Specific PCR.

Sperfel, Jean Marc; Sarfati, Claudine; Liguory, Olivier; Caroff, B.; Dumoutier, Nadine; Gueglio, B.; Billaud, Éric; Raffi, François; Molina, Jean‐Michel; Miégeville, M.; Derouin, Francis

doi:10.1111/j.1550-7408.1997.tb05792.x

Cited by 106 publications

(64 citation statements)

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“…As the common environmental source of microsporidia included ditch water and other stagnant water bodies, three common species of microsporidia that infect humans have been detected in the water samples. 1,2,25 The cluster infection in our cases may be attributed to exposure to contaminated soil during the contact sport in this season. Although the outbreak of our cases occurs in April (not the usual peak season), there is evidence that there is higher than usual rainfall in April this year (2012) in Singapore (http://www.weather.gov.sg/wip/web/ ASMC/Regional_Weather/Regional_Rainfall/ Peninsular_Malaysia_Singapore).…”

Section: Discussionmentioning

confidence: 96%

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Outbreak of microsporidial keratoconjunctivitis with rugby sport due to soil exposure

Kwok

Tong

Tang

et al. 2013

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Section: Discussionmentioning

confidence: 96%

“…1,2 Three species of microsporidia are believed to cause most cases of the infection, namely Enterocytozoon bieneusi, Septata intestinalis and Encephalitozoon hellem. 3 They infect the enterocytes of the small intestine and are associated with chronic diarrhoea.…”

Section: Introductionmentioning

confidence: 99%

Outbreak of microsporidial keratoconjunctivitis with rugby sport due to soil exposure

Kwok

Tong

Tang

et al. 2013

Eye

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“…Is man a natural reservoir for the parasite or an accidental host in whom the infection becomes possible because of the deterioration of his immune system? For instance, after being identified in humans, spores of E. bieneusi were detected in numerous animal species, especially mammals but also birds, and in environmental source such as surface water, swimming pools or rivers (Coupe et al, 2006;Dowd et al, 1998;Fournier et al, 2000;Haro et al, 2006;Haro et al, 2005;Santin et al, 2004;Sparfel et al, 1997). Analysis of the sequence of the 243-bp internal transcribed spacer rRNA gene has revealed a considerable genetic variation within E. bieneusi isolates of human and animal origins.…”

Section: Genotypic Diversity and Host Specificitymentioning

confidence: 99%

Enterocytozoon bieneusiin human and animals, focus on laboratory identification and molecular epidemiology

2008

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ummary :Human microsporidian infections have emerged following the onset of the AIDS pandemic. Microsporidia are unicellular eukaryotic parasites that form spores. They are an exceptionally diverse group of parasites that infect a wide range of eukaryotic cells in numerous invertebrate and vertebrate hosts. Of the 14 species newly described as pathogens in human, Enterocytozoon bieneusi, which causes gastrointestinal diseases, is the most common agent of human infections. In the past fifteen years, E. bieneusi was also identified in environmental sources, especially in surface water, as well as in wild, domestic and farm animals. These findings raised concerns for waterborne, foodborne and zoonotic transmission. Molecular analyses of the 243-bp internal Transcribed spacer-(ITS) of the rRNA gene have revealed a considerable genetic variation within E. bieneusi isolates of human and animal origins, supporting the potential for zoonotic transmission. The focus of this revue is to present and discuss recent advances in diagnosis and zoonotic potential of E. bieneusi infections.

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“…Microsporidian spores are commonly found in surface water and human pathogenic Microsporidia have been found in municipal water supplies, tertiary sewage effluent and ground water (Avery and Undeen, 1987;Sparfel et al, 1997;Dowd et al, 1998;Cotte et al, 1999). It is possible that many of the Microsporidia are zoonotic human infections.…”

Section: Overviewmentioning

confidence: 99%

The microsporidian polar tube: A highly specialised invasion organelle

Weiss

2005

International Journal for Parasitology

125

111

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All of the members of the Microsporidia possess a unique, highly specialised structure, the polar tube. This article reviews the available data on the organisation, structure and function of this invasion organelle. It was over 100 years ago that Thelohan accurately described the microsporidian polar tube and the triggering of its discharge. In the spore, the polar tube is connected at the anterior end, and then coils around the sporoplasm. Upon appropriate environmental stimulation the polar tube rapidly discharges out of the spore pierces a cell membrane and serves as a conduit for sporoplasm passage into the new host cell. The mechanism of germination of spores, however, remains to be definitively determined. In addition, further studies on the characterisation of the early events in the rupture of the anterior attachment complex, eversion of the polar tube as well as the mechanism of host cell attachment and penetration are needed in order to clarify the function and assembly of this structure. The application of immunological and molecular techniques has resulted in the identification of three polar tube proteins referred to as PTP1, PTP2 and PTP3. The interactions of these identified proteins in the formation and function of the polar tube remain to be determined. Data suggest that PTP1 is an O-mannosylated glycoprotein, a post-translational modification that may be important for its function. With the availability of the Encephalitozoon cuniculi genome it is now possible to apply proteomic techniques to the characterisation of the components of the microsporidian spore and invasion organelle.

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Detection of Microsporidia and Identification of Enterocytozoon bieneusi in Surface Water by Filtration Followed by Specific PCR.

Cited by 106 publications

References 3 publications

Outbreak of microsporidial keratoconjunctivitis with rugby sport due to soil exposure

Outbreak of microsporidial keratoconjunctivitis with rugby sport due to soil exposure

Enterocytozoon bieneusiin human and animals, focus on laboratory identification and molecular epidemiology

The microsporidian polar tube: A highly specialised invasion organelle

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