Detection of micrometastasis in lymph nodes of oral squamous cell carcinoma: A comparative study

Thakare, Eesha; Gawande, Madhuri; Chaudhary, Minal; Seralathan, Mohil; Krishnamurthy, K. S.

doi:10.4103/0973-029x.125202

Cited by 15 publications

(23 citation statements)

References 20 publications

(38 reference statements)

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“…The five-year survival rate of patients largely depends on the tumour stage at the time of diagnosis, decreasing from 50% in patients without the presence of lymph node metastasis to 90% with nodal metastasis [10–12]. Approximately 30% of patients with OSCC, who present without any clinical or radiographic evidence of regional disease, in fact harbour occult cervical metastasis, which is detected microscopically – divided into three types: macrometastases (> 2–3 mm in the largest dimension), micrometastases (< 2–3 mm in the largest dimension), and isolated tumour cells (individual or small clusters of tumour cells within the lymph node sinuses < 0.2 mm).…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 30% of patients with OSCC, who present without any clinical or radiographic evidence of regional disease, in fact harbour occult cervical metastasis, which is detected microscopically – divided into three types: macrometastases (> 2–3 mm in the largest dimension), micrometastases (< 2–3 mm in the largest dimension), and isolated tumour cells (individual or small clusters of tumour cells within the lymph node sinuses < 0.2 mm). The size and type of metastasis may influence the prognostic value of nodal metastases [12, 13]. Thus detection of tumour deposits within the lymph nodes is critical for patient care.…”

Section: Discussionmentioning

confidence: 99%

“…Although cytokeratins have been consistently used as immunohistochemical markers for detection of nodal metastases, they are not very specific. Reticulum cells, ectopic salivary gland inclusions, mesothelial inclusions, and metastatic thyroid follicles can show cytokeratin positivity [12, 14]. Several studies have also found inconsistencies in the precise cytokeratin to be analysed [7].…”

Section: Discussionmentioning

confidence: 99%

“…Using cytokeratins as markers of micrometastasis detection in HNSCC cases, various authors have shown that the detection rate varies from 1.3% to 68% [4, 12, 18]. Patel et al [7] evaluated formalin-fixed, paraffin-embedded, anonymised tissue sections of non-metastatic (N0) and metastatic (N+) human cervical lymph node biopsies from patients diagnosed with HNSCC for expression of DSG3 and cytokeratin.…”

Section: Discussionmentioning

confidence: 99%

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Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma

Nagvekar¹,

Spadigam²,

Dhupar³

2016

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Aim of the studyDespite advances in surgical and radiotherapy techniques, the presence of lymph node metastasis drastically decreases the survival rate of patients with primary oral squamous cell carcinoma (OSCC). Thus the accurate pathological staging of the neck is critical. Desmoglein 3 (DSG3), a desmosomal cadherin protein is said to be highly expressed in head and neck squamous cell carcinoma (HNSCC) and in metastatic cervical lymph nodes, but absent in non-invaded nodes. With an aim to improve the sensitivity of tumour cell detection, we investigated the potential of DSG3 as an immunohistochemical marker for the detection of occult lymph node metastasis in patients with primary OSCC.Material and methodsForty-seven lymph node specimens from 10 patients who underwent neck dissection for primary OSCC were immunostained with DSG3.ResultsThe DSG3 positivity was noted in the six positive lymph nodes. However, when using DSG3 as an immunohistochemical marker, no additional micrometastatic deposits were evident in the histologically negative nodes. Interestingly, tumour marker DSG3-positive macrophages could be identified within the subcapsular sinuses, medullary sinuses, and the interfollicular areas.ConclusionsOur findings suggest that although DSG3 is overexpressed in HNSCC, it is not specific and may not prove to be a potent immunohistochemical marker to detect micrometastasis. The role of tumour marker-positive macrophages within the lymph nodes needs to be investigated further.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma

Nagvekar¹,

Spadigam²,

Dhupar³

2016

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“…21 Also, our preliminary data suggest that level I can be involved radiographically but not clinically and can also be occult.…”

Section: Discussionmentioning

confidence: 99%

Feasibility and relevance of level I substation node counts in oropharyngeal carcinoma

et al. 2016

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Let‐7b‐5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377

Liu

Tan

et al. 2019

Cell Biology International

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KIAA1377 has been found to be linked with lymph node metastasis in esophageal squamous cell carcinoma (SCC) in our previous study; however, the regulation of KIAA1377 remains far from understood. Herein, to understand the regulation of KIAA1377 from the angle of microRNA (miRNA)–messenger RNA (mRNA) modulation in the setting of SCC cells, the basal level of KIAA1377 was determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot analysis in KYSE‐150 and HeLa cells; biological roles of KIAA1377 contributing in the proliferation, migration, and invasion were evaluated using 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT), wound‐healing and Transwell assays, respectively, after KIAA1377 was knocked out mediated by the CRISPR‐Cas9 system. Bioinformatic prediction revealed that let‐7b‐5p was a putative miRNA regulating KIAA1377, which was ensuingly validated by the luciferase reporter assay; after which, variation of KIAA1377 expression was further verified by qRT‐PCR and western blot analysis. Moreover, the biological roles of let‐7b‐5p in proliferation, migration, and invasion of KYSE‐150 and HeLa cells were also evaluated. It was exhibited that KIAA1377 was able to promote the proliferation and motility of both KYSE‐150 and HeLa cells, which can be reverted by re‐expression of let‐7b‐5p. The luciferase reporter assay verified that let‐7b‐5p can diametrically target KIAA1377. Collectively, our data demonstrated that let‐7b‐5p can directly but negatively regulate KIAA1377 in SCC cell lines, Ecal109, and HeLa cells.

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Detection of micrometastasis in lymph nodes of oral squamous cell carcinoma: A comparative study

Cited by 15 publications

References 20 publications

Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma

Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma

Feasibility and relevance of level I substation node counts in oropharyngeal carcinoma

Let‐7b‐5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377

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