Detection of microcystin contamination by the measurement of the variability of the in vivo chlorophyll fluorescence in aquatic plant Lemna gibba

Saqrane, S.; Ghazali, Issam El; Oudra, Brahim; Bouarab, L.; Dekayir, S.; Mandi, Laila; Ouazzani, Naaila; Vasconcelos, Vı́tor

doi:10.1016/j.toxicon.2008.10.004

Cited by 14 publications

(5 citation statements)

References 18 publications

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“…+ presence of the isoenzyme; ↑ increase of intensity as compared to control; ↓ decrease in intensity as compared to control. Pflugmacher et al, 2007;Järvenpää et al 2007;Saqrane et al, 2009). Pflugmacher (2002) established that the sensitivity of Ceratophyllum demersum to MC-LR is higher than of macroalgae (Cladophora sp.)…”

Section: Discussionmentioning

confidence: 99%

Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: Ecotoxicological measurements in real environment with real microcystin exposure

Ujvárosi

Riba

Garda

et al. 2019

Science of The Total Environment

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Overproduction of toxic cyanobacteria is a type of harmful algal blooms (HABs). The heptapeptide microcystins (MCs) are one of the most common cyanotoxins. There is increasing research concerning the effects of MCs on growth and physiology of vascular plants, however there is a lack of studies on their direct effects on aquatic macrophytes in the real environment. Here we report the occurrence of a MC producing HAB in Lake Bárdos, Hungary in 2012 with harmful effects on cytological, histological and biochemical parameters of Ceratophyllum submersum (soft hornwort) plants naturally growing at the blooming site. Blue-Green Sinapis Test (BGST) showed high toxicity of HAB samples. Cell-free water samples contained a significant amount of MCs (7.31 ± 0.17 μg L −1 ) while C. submersum plants contained 1.01 ± 0.21 μg g DW −1 MCs. Plants showed significant increases of protein content and decreases of anthocyanin content and carotenoid/chlorophyll ratio, indicating physiological stress-as compared to plants from the control (MC free) sampling site of the same water body. Histological and cytological studies showed (i) radial swelling and the abnormal formation of lateral buds at the shoot tip leading to abnormal development; (ii) the fragmentation of nuclei as well as accumulation of phenolics in the nucleus indicating that the HAB induced cell death and stress reactions at the nuclear level. The most relevant effect was the increase of histone H3 phosphorylation in metaphase chromosomes: since MCs are strong inhibitors of protein phosphatases, this alteration is related to the biochemical targets of these toxins. The HAB decreased peroxidase activity, but increased nuclease and protease activities, showing the decreased capacity of plants to face biotic stress and as the cytological changes, the induction of cell death. This study is one of the first to show the complex harmful changes in aquatic plants that co-exist with HABs.

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Section: Discussionmentioning

confidence: 99%

Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: Ecotoxicological measurements in real environment with real microcystin exposure

Ujvárosi

Riba

Garda

et al. 2019

Science of The Total Environment

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“…The same has been previously concluded by Ferrante and Maggiore (2007) when they compared chlorophyll fluorescence measurements to anthocyanins, carotenoids and phenolic compounds determination as means to evaluate the quality status of leafy vegetables such as the lettuce Valeriana. Saqrane et al (2009b) has clearly demonstrated the Chl-fluorescence sensitivity to cyanotoxins stress induced in the aquatic plant L. gibba. So, it could be applied to monitor the aquatic ecosystem quality using Lemna as the biotest organism for water quality assessment under aquatic environment preservation programs.…”

Section: Detection Of Microcystins Contamination and Oxidative Stressmentioning

confidence: 96%

“…However, to the best of our knowledge, the use of variable fluorescence as a biophysical indicator of cyanotoxins stress has been rarely reported, particularly in aquatic plants. Only one work was recently published to test the potential use of Chl-fluorescence as a simple biophysical method to evaluate stress induced by cyanotoxins in the aquatic plant L. gibba, following exposure to microcystins (10-300 µg/ml equivalent MC-LR) during 5 h under laboratory conditions (Saqrane et al, 2009b). For terrestrial plants, also a single work that was done to evaluate the physiological state of the photosynthetic apparatus of Brassica oleracea var.…”

Section: Detection Of Microcystins Contamination and Oxidative Stressmentioning

confidence: 99%

Cyanobacterial toxins: A short review on phytotoxic effect in an aquatic environment

Saqrane¹

2012

Afr. J. Environ. Sci. Technol.

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Cyanobacteria are photosynthetic prokaryotes which frequently form blooms in eutrophic water bodies. Some species of cyanobacteria are able to produce toxins (cyanotoxins) that can cause aquatic environment and diverse organisms living there to be at a serious risk. One of the more serious impacts of eutrophication on aquatic ecosystems is the disappearance of submerged macrophytes and the shift to a phytoplankton-dominated state. Hence, cyanobacterial blooms may be of significant negative ecological impact. This may represent a sanitary risk due to toxin bioaccumulation and biotransfer through the food chain. So, with the increasing number of new researches made on this subject, we propose this paper to review clearly many recent and original reports that have demonstrated the effects of cyanotoxins on some biological and physiological pathways in different aquatic plants.

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“…To date, there are no convincing data on the possible detrimental effects of MCs on the structure of already existing thylakoid membranes or any other internal membrane types of plastid. These alterations are expected due to (i) the abundance of data on the inhibitory effects of MCs and other cyanotoxins on photosynthetic oxygen production and photochemical activities, processes that occur at the level of thylakoids and (ii) the involvement of the PP2A–target of MCs in oxidative stress responses in chloroplasts [ 44 , 45 , 46 ]. However, there is indirect evidence for the interference of MCs with thylakoid biogenesis .…”

Section: Alterations Induced By Cyanotoxins On Peculiar Plant Structuresmentioning

confidence: 99%

Subcellular Alterations Induced by Cyanotoxins in Vascular Plants—A Review

Máthé

M-Hamvas

Vasas

et al. 2021

Plants

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Phytotoxicity of cyanobacterial toxins has been confirmed at the subcellular level with consequences on whole plant physiological parameters and thus growth and productivity. Most of the data are available for two groups of these toxins: microcystins (MCs) and cylindrospermopsins (CYNs). Thus, in this review we present a timely survey of subcellular cyanotoxin effects with the main focus on these two cyanotoxins. We provide comparative insights into how peculiar plant cellular structures are affected. We review structural changes and their physiological consequences induced in the plastid system, peculiar plant cytoskeletal organization and chromatin structure, the plant cell wall, the vacuolar system, and in general, endomembrane structures. The cyanotoxins have characteristic dose-and plant genotype-dependent effects on all these structures. Alterations in chloroplast structure will influence the efficiency of photosynthesis and thus plant productivity. Changing of cell wall composition, disruption of the vacuolar membrane (tonoplast) and cytoskeleton, and alterations of chromatin structure (including DNA strand breaks) can ultimately lead to cell death. Finally, we present an integrated view of subcellular alterations. Knowledge on these changes will certainly contribute to a better understanding of cyanotoxin–plant interactions.

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Detection of microcystin contamination by the measurement of the variability of the in vivo chlorophyll fluorescence in aquatic plant Lemna gibba

Cited by 14 publications

References 18 publications

Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: Ecotoxicological measurements in real environment with real microcystin exposure

Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: Ecotoxicological measurements in real environment with real microcystin exposure

Cyanobacterial toxins: A short review on phytotoxic effect in an aquatic environment

Subcellular Alterations Induced by Cyanotoxins in Vascular Plants—A Review

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