Detection of methicillin-resistant coagulase-negative staphylococci harboring the class A mec complex by MALDI-TOF mass spectrometry

Schuster, Dominik; Josten, Michaele; Janssen, Kathrin; Bodenstein, Isabel; Albert, Cathrin; Schallenberg, Anna; Gajdiss, Mike; Sib, Esther; Szekat, Christiane; Kehl, Katja; Parčina, Marijo; Hischebeth, Gunnar; Bierbaum, Gabriele

doi:10.1016/j.ijmm.2018.05.001

Cited by 21 publications

(16 citation statements)

References 33 publications

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“…This could also include applications such as the direct differentiation of MRSA and MSSA, VIM and SPM-type β-lactamase-producing Pseudomonas aeruginosa. 10,17 Several other studies have proposed a new method called the MALDI Biotyper-Antibiotic Susceptibility Test Rapid Assay (MBT-ASTRA) to differentiate antimicrobial-resistant and sensitive strains in a short time of 2-4 h. [18][19][20] MBT-ASTRA experiments have used a relative growth (RG) algorithm to calculate the ratio of area under the curves (AUCs) for bacteria mass spectra when the test strains were incubated with and without antibiotics. The approach allowed the quantitative detection of bacteria growth under different concentrations of antibiotics using MALDI-TOF MS. Also, an important aspect of this method allows the resistance and susceptibility of different bacterial strains to be distinguished at applicable breakpoint concentrations of various antibiotics.…”

Section: Introductionmentioning

confidence: 99%

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A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a novel method that can be used to identify pathogens and has potential applications in the detection of drug-resistant bacteria. Purpose: To evaluate the ability of a MALDI-TOF MS-based broth micro-dilution method in detecting the minimum inhibitory concentration (MIC) values of Klebsiella pneumoniae to ceftriaxone and imipenem. Materials and Methods: Sixty strains of K. pneumoniae with different levels of resistance to carbapenems and cephalosporins were randomly collected. The 0.5 McFarland (Mc) concentration of the bacterial suspension was inoculated in cation-adjusted Mueller-Hinton broth (CAMHB) with a final cell turbidity of 5×10 5 CFU/mL. The broth was incubated with serial concentrations of antibiotics. After centrifuging the bacterial suspensions, the lysed cells were analyzed by MALDI-TOF MS to identify the growth-promoting or inhibitory effects on K. pneumoniae. The molecular mechanisms of resistance were investigated by PCR and DNA sequencing analysis. Results: The expression of known resistance genes (blaKPC, blaFOX, blaDHA, blaCTX-M and blaTEM) was detected in the 30 carbapenems-resistant strains. The agreement between the MIC values derived from the MALDI-TOF MS analysis and from the broth microdilution method was 61.7% for ceftriaxone and 71.7% for imipenem. According to the Clinical and Laboratory Standards Institute (CLSI) breakpoint of resistance to ceftriaxone and imipenem, the 60 isolates were accurately classified as resistant or susceptible isolates with 100% sensitivity and 100% specificity. Conclusion:The transmission and infection of multidrug-resistant bacteria could be better managed and treated with the rapid identification of strains and antimicrobial susceptibility. A MALDI-TOF MS-based susceptibility test could be used to identify resistance of K. pneumoniae within a short time-frame. This approach could potentially be used as a supplementary antimicrobial susceptibility test that could be investigated on more bacterial species combined with different antibiotics.

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Section: Introductionmentioning

confidence: 99%

“…This could also include applications such as the direct differentiation of MRSA and MSSA, VIM and SPM-type β-lactamase-producing Pseudomonas aeruginosa . 10 , 17 …”

Section: Introductionmentioning

confidence: 99%

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Wang

Song

2021

IDR

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“…The heterogeneous nature of methicillin-resistance in Staphylococcus aureus has hindered the possibility to classify methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) into two clearly distinguishable groups based on clustering analysis of the spectral profiles of individual isolates (Wang et al, 2013). A partial success was achieved by establishing a method, which relies on the production of a phenol-soluble protein toxin (PSM-mec) by a subset of MRSA strains (Chatterjee et al, 2011), which is detectable by MALDI-TOF MS as a 2415 ± 2.00 m/z peak and may be present in up to half of MRSA isolates (Rhoads et al, 2016;Schuster et al, 2018). The PSM-mec peptide does not cause methicillin-resistance and its biological function is unknown, but its expression is associated with methicillin-resistance.…”

Section: Identification Of Biomarkers Associated With Drug-resistancementioning

confidence: 99%

“…The software "MBT subtyping module" (Bruker Daltonics), has been developed to detect PSM-mec in the mass spectrum of S. aureus isolates, providing an indirect evidence of methicillinresistance ( Figure 2). Although the presence of PSM-mec is associated with methicillin-resistance also in coagulase-negative staphylococci (CoNS), the majority of those does not produce it (Schuster et al, 2018), thus being of limited usefulness to detect methicillin-resistant CoNS.…”

Section: Identification Of Biomarkers Associated With Drug-resistancementioning

confidence: 99%

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

Florio

Baldeschi

Rizzato

et al. 2020

Front. Cell. Infect. Microbiol.

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Several MALDI-TOF MS-based methods have been proposed for rapid detection of antimicrobial resistance. The most widely studied methods include assessment of β-lactamase activity by visualizing the hydrolysis of the β-lactam ring, detection of biomarkers responsible for or correlated with drug-resistance/non-susceptibility, and the comparison of proteomic profiles of bacteria incubated with or without antimicrobial drugs. Antimicrobial-resistance to a number of antibiotics belonging to different classes has been successfully tested by MALDI-TOF MS in a variety of clinically relevant bacterial species including members of Enterobacteriaceae family, non-fermenting Gram-negative bacteria, Gram-positive cocci, anaerobic bacteria and mycobacteria, opening this field to further clinically important developments. Early detection of drug-resistance by MALDI-TOF MS can be particularly helpful for clinicians to streamline the antibiotic therapy for a better outcome of patients with systemic infection, in all cases where a prompt and effective antibiotic treatment is essential to preserve organ function and/or patient survival.

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“…The detection of the biomarker proved to be highly specific – but only for isolates harboring SCC mec types II and III while generally lacking in isolates harboring SCC mec types I, IV, or XI (Rhoads et al, 2016). Therefore, while the presence of a certain peak in a spectrum is indicative of MRSA, its absence must not be interpreted as indication of MSSA (Schuster et al, 2018). Differences in peak patterns in this lower mass range can be used to type MRSA isolates for epidemiological purposes (Lindgren et al, 2018).…”

Section: Detection Of Biomarkers Associated With Antibiotic Resistancementioning

confidence: 99%

One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

Welker

Belkum

2019

Front. Microbiol.

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The two main pillars of clinical microbiological diagnostics are the identification of potentially pathogenic microorganisms from patient samples and the testing for antibiotic susceptibility (AST) to allow efficient treatment with active antimicrobial agents. While routine microbial species identification is increasingly performed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), routine AST still largely relies on conventional and molecular techniques such as broth microdilution or disk and gradient diffusion tests, PCR and automated variants thereof. However, shortly after the introduction of MALDI-TOF MS based routine identification, first attempts to perform AST on the same instruments were reported. Today, a number of different approaches to perform AST with MALDI-TOF MS and other MS techniques have been proposed, some restricted to particular microbial taxa and resistance mechanisms while others being more generic. Further, while some of the methods are in a stage of proof of principles, others are already commercialized. In this review we discuss the different principal approaches of mass spectrometry based AST and evaluate the advantages and disadvantages compared to conventional and molecular techniques. At present, the possibility that MS will soon become a routine tool for AST seems unlikely – still, the same was true for routine microbial identification a mere 15 years ago.

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Detection of methicillin-resistant coagulase-negative staphylococci harboring the class A mec complex by MALDI-TOF mass spectrometry

Cited by 21 publications

References 33 publications

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

A Rapid Antimicrobial Susceptibility Test for Klebsiella pneumoniae Using a Broth Micro-Dilution Combined with MALDI TOF MS

Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area

One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

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