Detection of methicillin-resistant coagulase-negative staphylococci and PVL/mecA genes in cefoxitin-susceptible Staphylococcus aureus (t044/ST80) from unpasteurized milk sold in stores in Djelfa, Algeria

Chenouf, Nadia Safia; Mama, Olouwafemi Mistourath; Messaï, Chafik Redha; Ruiz-Ripa, Laura; Fernández-Fernández, Rosa; Carvalho, Isabel; Zitouni, Abdelghani; Hakem, Ahcène; Torres, Carmén

doi:10.3168/jds.2020-19270

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…Mainly described as clinical and subclinical mastitis strains, ST71 belongs to the same lineage as ST97, with more and more isolates demonstrated to be MRSA (Cormican and Keane, 2018), and in the present work they were present in bovine isolates. ST479 was only seen in milk samples (Chenouf et al, 2021); ST115 was consistent with bovine isolates (Smith et al, 2005) and ST3183 was recently identified in raw milk samples (McMillan et al, 2016). ST20 was cited in strains bovines and humans (Aung et al, 2019), and ST133 was cited in several hosts and environmental samples (Roberts et al, 2013).…”

Section: Gene Profiles Mlstmentioning

confidence: 70%

Comparative genomics study of Staphylococcus aureus isolated from cattle and humans reveals virulence patterns exclusively associated with bovine clinical mastitis strains

Rodrigues

Pizauro²,

Varani³

et al. 2022

Front. Microbiol.

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Staphylococcus aureus causes nosocomial and intramammary infections in humans and cattle, respectively. A large number of virulence factors are thought to play important roles in the pathogenesis of this bacterium. Currently, genome-wide and data-analysis studies are being used to better understand its epidemiology. In this study, we conducted a genome wide comparison and phylogenomic analyses of S. aureus to find specific virulence patterns associated with clinical and subclinical mastitis strains in cattle and compare them with those of human origin. The presence/absence of key virulence factors such as adhesin, biofilm, antimicrobial resistance, and toxin genes, as well as the phylogeny and sequence type of the isolates were evaluated. A total of 248 genomes (27 clinical mastitis, 43 subclinical mastitis, 21 milk, 53 skin-related abscesses, 49 skin infections, and 55 pus from cellulitis) isolated from 32 countries were evaluated. We found that the cflA, fnbA, ebpS, spa, sdrC, coa, emp, vWF, atl, sasH, sasA, and sasF adhesion genes, as well as the aur, hglA, hglB, and hglC toxin genes were highly associated in clinical mastitis strains. The strains had diverse genetic origins (72 protein A and 48 sequence types with ST97, ST8 and ST152 being frequent in isolates from clinical mastitis, abscess, and skin infection, respectively). Further, our phylogenomic analyses suggested that zoonotic and/or zooanthroponotic transmission may have occurred. These findings contribute to a better understanding of S. aureus epidemiology and the relationships between adhesion mechanisms, biofilm formation, antimicrobial resistance, and toxins and could aid in the development of improved vaccines and strain genotyping methods.

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Section: Gene Profiles Mlstmentioning

confidence: 70%

Comparative genomics study of Staphylococcus aureus isolated from cattle and humans reveals virulence patterns exclusively associated with bovine clinical mastitis strains

Rodrigues

Pizauro²,

Varani³

et al. 2022

Front. Microbiol.

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“…However, recent investigations have shown the increasing role of CoNS in causing antibiotic-resistant infections [ 55 , 56 ]. In a previous study conducted in Algeria, which focused on unpasteurized cow’s milk, three isolated CoNS were resistant to methicillin, and all were mecA -positive [ 57 ]. A few studies carried out on CoNS in livestock have revealed that food-producing animals constitute a large reservoir of multiresistant CoNS [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria

Yousfi,

Oueslati,

Daaboul

et al. 2024

Microorganisms

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Abscesses represent the most prominent emerging problem in the red meat industry, leading to great economic constraints and public health hazards. Data on etiological agents present in these purulent lesions in Algeria are very scarce. The aim of this study was to identify the bacteria responsible for these abscesses and to determine their antibiotic susceptibility profiles. A total of 123 samples of abscesses from 100 slaughtered sheep and 23 slaughtered cattle were cultured in several media. A total of 114 bacterial isolates were cultured from 103 abscesses. Bacteria were identified using MALDI–TOF, and antibiotic susceptibility was determined by the disk diffusion method on Mueller–Hinton agar. A total of 73.6% (n = 84) corresponded to Enterobacterales, of which four were multidrug-resistant (MDR). These isolates, together with Staphylococcus aureus, coagulase negative Staphylococci, and seven randomly chosen susceptible Escherichia coli isolates, were further characterized using WGS. Resistome analysis of the four MDR Enterobacterales isolates revealed the presence of OXA-48 carbapenemase in two Klebsiella pneumoniae ST985 and one E. coli ST10 isolates and a CTX-M-15 ESBL in one E. coli isolate ST1706. Two coagulase-negative Staphylococci isolates were found to carry the mecA gene. WGS showed the presence of different resistance genes and virulence genes. Our study revealed 5% of MDR Enterobacterales (including ESBLs and carbapenemases) identified from abscesses, thus urging the need for abscess monitoring in slaughterhouses.

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“…It is s well known that staphylococci, including methicillin-resistant strains, are an important cause of mastitis in dairy cows, ewes, buffalo and camels [ 114 , 147 , 148 ]. The bacteria are subsequently excreted into the milk without organoleptic alteration, allowing them to spread through the food chain if the milk is not treated properly [ 59 , 135 , 146 , 148 , 149 ]. MRSA has been reported in both raw and pasteurized milk from cattle and camels [ 115 , 135 , 149 , 150 ] ( Table 3 ).…”

Section: Methicillin-resistant Staphylococci (Mrs) In Animalsmentioning

confidence: 99%

“…The bacteria are subsequently excreted into the milk without organoleptic alteration, allowing them to spread through the food chain if the milk is not treated properly [ 59 , 135 , 146 , 148 , 149 ]. MRSA has been reported in both raw and pasteurized milk from cattle and camels [ 115 , 135 , 149 , 150 ] ( Table 3 ). The traditional dairy products, which occupy an important place in the diet in Arab countries and particularly in the North African countries (Algeria, Tunisia, Morocco), have also been shown to carry MRSA [ 151 ].…”

Section: Methicillin-resistant Staphylococci (Mrs) In Animalsmentioning

confidence: 99%

“…The traditional dairy products, which occupy an important place in the diet in Arab countries and particularly in the North African countries (Algeria, Tunisia, Morocco), have also been shown to carry MRSA [ 151 ]. The MRSA types belonged to well-known human types (ST80, ST8, ST4114, ST4120) [ 74 , 135 , 149 ], though some, like the ST80 strains highly prevalent in the Mediterranean region, have also been detected in animals and food products [ 61 , 149 ]. The ST8 lineage, detected in raw milk in Algeria [ 135 ], is related to the USA300 clone, which is predominant in the United States, South America [ 152 ] and the Caribbean [ 153 ].…”

Section: Methicillin-resistant Staphylococci (Mrs) In Animalsmentioning

confidence: 99%

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Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Methicillin-Resistant Staphylococci and Mammaliicoccus (MRNaS) Associated with Animals and Food Products in Arab Countries: A Review

Belhout

Elgroud

Butaye

2022

Veterinary Sciences

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The prevalence of methicillin resistance in staphylococci has been increasing globally and is currently one of the major public health concerns. In particular, treating infections caused by staphylococci with acquired antimicrobial resistance is problematic, as their treatment is more difficult. The resistance is found both in human and animal staphylococcal strains. Methicillin-resistant staphylococci (MRS) have also been increasingly reported in wildlife. In Arab countries, MRS has been detected in food producing animals and food products; however, the risk this poses is somewhat unclear, and still a significant lack of information on the trend and distribution of these pathogens in these countries, which have a specific ecosystem (desert) and traditions (Muslim culture). In this manuscript, we aim to provide an overview of the prevalence and the major MRS clonal lineages circulating in these specific countries and compare to them other situations with different ecosystems and cultures.

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Detection of methicillin-resistant coagulase-negative staphylococci and PVL/mecA genes in cefoxitin-susceptible Staphylococcus aureus (t044/ST80) from unpasteurized milk sold in stores in Djelfa, Algeria

Cited by 11 publications

References 48 publications

Comparative genomics study of Staphylococcus aureus isolated from cattle and humans reveals virulence patterns exclusively associated with bovine clinical mastitis strains

Comparative genomics study of Staphylococcus aureus isolated from cattle and humans reveals virulence patterns exclusively associated with bovine clinical mastitis strains

Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria

Methicillin-Resistant Staphylococcus aureus (MRSA) and Other Methicillin-Resistant Staphylococci and Mammaliicoccus (MRNaS) Associated with Animals and Food Products in Arab Countries: A Review

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