Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system

Avlami, A.; Bekris, S.; Ganteris, G; Kraniotaki, Eleni; Malamou-Lada, Eleni; Orfanidou, Maria; Paniara, Olga; Pantazatou, Angeliki; Papagiannitsis, Costas C.; Platsouka, E.; Stefanou, Ioanna; Tzelepi, E.; Vagiakou, Helen; Miriagou, Vivì

doi:10.1016/j.mimet.2010.08.014

Cited by 38 publications

(24 citation statements)

References 9 publications

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“…The Hyplex Super Bug ID system (Amplex Biosystems GmbH, Giessen, Germany) for the detection of carbapenemases is based on a multiplex endpoint PCR followed by enzyme-linked immunosorbent assay (ELISA) hybridization (171). Although it has not been tested directly on stool specimens, this NAAT showed 98% sensitivity and specificity for VIM-producing CPE when used on DNA extracted from clinical specimens, including blood, urine, pus, and respiratory samples, from Greece (172). Another multiplex endpoint PCR was developed by Voets et al and allows the detection of a wide range of resistance genes (173).…”

Section: Screening Methods To Detect Fecal Carriage Of Cposmentioning

confidence: 99%

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

et al. 2016

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SUMMARYCarbapenemases have become a significant mechanism for broad-spectrum β-lactam resistance inEnterobacteriaceaeand other Gram-negative bacteria such asPseudomonasandAcinetobacterspp. Intestinal carriage of carbapenemase-producing organisms (CPOs) is an important source of transmission. Isolation of carriers is one strategy that can be used to limit the spread of these bacteria. In this review, we critically examine the clinical performance, advantages, and disadvantages of methods available for the detection of intestinal carriage of CPOs. Culture-based methods (Centers for Disease Control and Prevention [CDC] protocols, chromogenic media, specialized agars, and double-disk synergy tests) for detecting carriage of CPOs are convenient due to their ready availability and low cost, but their limited sensitivity and long turnaround time may not always be optimal for infection control practices. Contemporary nucleic acid amplification techniques (NAATs) such as real-time PCR, hybridization assays, loop-mediated isothermal amplification (LAMP), or a combined culture and NAAT approach may provide fast results and/or added sensitivity and specificity compared with culture-based methods. Infection control practitioners and clinical microbiologists should be aware of the strengths and limitations of available methods to determine the most suitable approach for their medical facility to fit their infection control needs.

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Section: Screening Methods To Detect Fecal Carriage Of Cposmentioning

confidence: 99%

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

et al. 2016

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“…The advantage of these PCRs is that they allow carbapenemaseencoding genes to be detected from clinical samples [180].…”

Section: Pcr Methodsmentioning

confidence: 99%

Detection of expanded-spectrum β-lactamases in Gram-negative bacteria in the 21st century

Al-Bayssari

Dabboussi

Hamzé

et al. 2015

Expert Review of Anti-infective Therapy

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Emerging β-lactamase-producing-bacteria (ESBL, AmpC and carbapenemases) have become a serious problem in our community due to their startling spread worldwide and their ability to cause infections which are difficult to treat. Diagnosis of these β-lactamases is of clinical and epidemiological interest. Over the past 10 years, several methods have been developed aiming to rapidly detect these emerging enzymes, thus preventing their rapid spread. In this review, we describe the range of screening and detection methods (phenotypic, molecular and other) for detecting these β-lactamases but also whole genome sequencing as a tool for detecting the genes encoding these enzymes.

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“…Befitting a rapid test, these assays can be utilized directly on patient samples; however, the ELISA-hybridization "modules" for consensus and specific detection of bla genes tend to be rather laborious and time consuming. The Hyplex MBL ID multiplex PCR-ELISA detects metallo-␤-lactamases bla VIM and bla IMP and was tested with clinical samples (urine, pus swabs, respiratory samples, and positive blood cultures) for bla VIM detection and showed sensitivity and specificity of 98% and 99%, respectively (2).…”

Section: Molecular Detection Of Esbl-harboring and Carbapenem-resistamentioning

confidence: 99%

Current Trends in Culture-Based and Molecular Detection of Extended-Spectrum-β-Lactamase-Harboring and Carbapenem-Resistant Enterobacteriaceae

et al. 2012

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The ever-expanding role of extended-spectrum-␤-lactamase (ESBL)-harboring and carbapenem-resistant Enterobacteriaceae in causing serious infections poses a grave public health threat because these organisms also tend to be multidrug resistant, for which very few (if any) antibiotic options remain available. Rapid detection of such panresistant organisms offers one of the best solutions to improve patient screening and hospital infection control practices as well as curb inappropriate antibiotic use. This review discusses and compares primarily the current state-of-the-art culture-based and molecular methods that are commercially available for detection or screening of ESBL-harboring and carbapenem-resistant Enterobacteriaceae.

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Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system

Cited by 38 publications

References 9 publications

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods

Detection of expanded-spectrum β-lactamases in Gram-negative bacteria in the 21st century

Current Trends in Culture-Based and Molecular Detection of Extended-Spectrum-β-Lactamase-Harboring and Carbapenem-Resistant Enterobacteriaceae

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