“…Because of the difficulty of preventing the decay of complement activity in commercially distributed horse serum and the growthinhibitory effect of the horse serum on Al. pneumoniae (18), this assay does not warrant a satisfactory level of sensitivity and often fails to give consistent results (17). In order to overcome these drawbacks, we developed an assay system employing an optimal amount of guinea pig complement in conjugation with gamma globulindepleted horse serum.…”
“…Because of the difficulty of preventing the decay of complement activity in commercially distributed horse serum and the growthinhibitory effect of the horse serum on Al. pneumoniae (18), this assay does not warrant a satisfactory level of sensitivity and often fails to give consistent results (17). In order to overcome these drawbacks, we developed an assay system employing an optimal amount of guinea pig complement in conjugation with gamma globulindepleted horse serum.…”
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