Detection of Meningioma in Dura Mater by Raman Spectroscopy

Koljenović, Senada; Schut, Tom C. Bakker; Vincent, Arnaud J P E; Kros, Johan M.; Puppels, Gerwin J.

doi:10.1021/ac0512599

Cited by 145 publications

(101 citation statements)

References 39 publications

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“…Biomolecules more abundant in control Fibroblasts 608 Cholesterol [56] 621 Phenylalanine (proteins) [57] 640,645 Proteins [43,56,58] 702 Cholesterol [56] 717-719 Phospholipids [43] 752-755 Proteins [59,60] 850 Amino acids [61] 957-960 Cholesterol [57] 1004 Phenylalanine (proteins) [62] 1128 Proteins (C-N bonds) [43] 1296,1302 CH 2 twist (lipids) [63,64] 1335-1339 CH 2 -CH 3 (proteins) [59] 1437-1451 CH 2 bend (lipids / proteins) [56,65] 1440-1444 Cholesterol [66] 1659 Cholesterol [66] 1669-1674 Cholesterol [56,66] Raman frequency (wavenumbers, cm…”

Section: Discussionmentioning

confidence: 99%

Raman spectroscopy of fibroblast cells from a Huntington's disease patient

Tsikritsis

Elfick

Downes

2016

Spectroscopy Letters

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We present a Raman spectroscopy case study of living fibroblast (skin) cells from a patient who developed Huntington's disease, with fibroblasts from a healthy volunteer as a control. Spectra were processed to remove cosmic rays, had a spectrum of the quartz substrate subtracted, and were flattened to remove cellular autofluorescence. We achieved an accuracy of 95% in discriminating individual cells, and assign spectral differences to (i) the reduction of cholesterol, (ii) the reduction of lipids, and (iii) an increase in beta-sheet proteins for fibroblasts with Huntington's disease. All these biochemical changes have been previously measured by other methods. Averages over all the cells in this study yield a difference which is extremely statistically significant [p < 0.0001]. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Raman spectroscopy of fibroblast cells from a Huntington's disease patient

Tsikritsis

Elfick

Downes

2016

Spectroscopy Letters

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“…Examples of clinical use of Raman spectroscopy being actively investigated are areas such as the evaluation of potentially detrimental pathogens (29), tissue foreign body (30), dental disease (31), and cancers (32,33). Clearly, the utility of Raman spectroscopy within human diseases has great potential, but certain obstacles must be overcome.…”

Section: Discussionmentioning

confidence: 99%

Fiberoptic Resonance Raman Spectroscopy to Measure Carotenoid Oxidative Breakdown in Live Tissues

Bentz¹,

Diaz²,

Ring³

et al. 2010

Cancer Prevention Research

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Based on compelling epidemiologic and corroboratory in vitro studies, carotenoids are thought to have great potential as dietary prevention against cancer. Yet, carotenoid-based chemopreventive trials have found very contradictory results. Definitive conclusions from these trials are hampered by an inability to accurately and safely measure carotenoids in specific tissues at risk of cancer development. Raman spectroscopy has been proposed as an optical technology with which to analyze various molecules in live tissues. One major obstacle that impedes the clinical use of this powerful technology is the lack of a fiberoptic Raman probe suitable for endoscopic tissue evaluation. A single-fiber resonance Raman Spectroscope capable of noninvasive "optical biopsies" to measure carotenoid concentrations in live tissues has been developed. The accuracy of this Raman instrument was confirmed by comparison with more standard methods of spectrophotometry and high-pressure liquid chromatography using solubilized β-carotene (BC) and BC-loaded cells before use in a small patient cohort. This Raman instrument detected intact BC as well as BC oxidative breakdown as a decrement of its Raman signal in cells. Use of the Raman instrument in our small cohort study showed its feasibility for measuring human tissues and raised some potentially intriguing possibilities about BC tissue pharmacokinetics and oxidative biology. Based on these results, our newly developed single fiberoptic resonance Raman instrument may provide a very useful method of measuring carotenoids and their oxidative breakdown within live tissue during future carotenoid chemopreventive trials. This proof-of-concept study provides the foundation to justify future validation of our Raman prototype.

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“…The hallmarks of SCI are cell death, demyelination, and aggregation of GAGs and CSPGs. Peak assignments were made based exclusively on the literature pertaining to Raman spectroscopy of the central nervous system (brain tissue), 22 GAGs, 10,23 CSPGs, 23,24 cholesterol, 22,25 and blood. 26 These are listed in Table 1.…”

Section: Journal Of Biomedical Opticsmentioning

confidence: 99%

Raman spectroscopic investigation of spinal cord injury in a rat model

et al. 2011

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Abstract. Raman spectroscopy was used to study temporal molecular changes associated with spinal cord injury (SCI) in a rat model. Raman spectra of saline-perfused, injured, and healthy rat spinal cords were obtained and compared. Two injury models, a lateral hemisection and a moderate contusion were investigated. The net fluorescence and the Raman spectra showed clear differences between the injured and healthy spinal cords. Based on extensive histological and biochemical characterization of SCI available in the literature, these differences were hypothesized to be due to cell death, demyelination, and changes in the extracellular matrix composition, such as increased expression of proteoglycans and hyaluronic acid, at the site of injury where the glial scar forms. Further, analysis of difference spectra indicated the presence of carbonyl containing compounds, hypothesized to be products of lipid peroxidation and acid catalyzed hydrolysis of glycosaminoglycan moieties. These results compared well with in vitro experiments conducted on chondroitin sulfate sugars. Since the glial scar is thought to be a potent biochemical barrier to nerve regeneration, this observation suggests the possibility of using near infrared Raman spectroscopy to study injury progression and explore potential treatments ex vivo, and ultimately monitor potential remedial treatments within the spinal cord in vivo. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Detection of Meningioma in Dura Mater by Raman Spectroscopy

Cited by 145 publications

References 39 publications

Raman spectroscopy of fibroblast cells from a Huntington's disease patient

Raman spectroscopy of fibroblast cells from a Huntington's disease patient

Fiberoptic Resonance Raman Spectroscopy to Measure Carotenoid Oxidative Breakdown in Live Tissues

Raman spectroscopic investigation of spinal cord injury in a rat model

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